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Microbiology Homework 8: Understanding Genetics and Pathogens in Microbiology - Prof. Tere, Assignments of Microbiology

A microbiology homework assignment focusing on the topics of chromosomes, genes, dna replication, genotype, phenotype, and various microbial genetics processes such as conjugation, transduction, translation, and transcription. Students are required to answer questions related to these topics, including the difference between chromosomes and genes, the process of dna replication, and the definitions of genotype and phenotype.

Typology: Assignments

2009/2010

Uploaded on 02/25/2010

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Microbiology Homework #8 Name ________________________
25 points- due ______________ VCC___________
CHAPTER 8- Microbial Genetics; Pathogens
(one point per number unless otherwise stated)
1. Explain what the difference is between a chromosome and a gene, which is longer?
2. Due to semiconservative DNA replication, each piece of DNA contains one _____________________
strand of DNA nucleotides and one __________________________ strand of DNA nucleotides.
3. Define the following words:
A. GENOTYPE:
B. PHENOTYPE:
4. Match the following terms: (2 points)
Conjugation Transduction Translation Plasmids
Transcription Binary Fission Transposons Transformation
___________________ The transfer of DNA from a donor to a recipient bacterial cell as naked
pieces of DNA in a solution
___________________ The process of asexual reproduction in bacterial cells
___________________ The process where mRNA is made from a gene
___________________ The transfer of DNA from a donor to a recipient bacterial cell through a
sex pilus
___________________ The transfer of DNA from a donor to a recipient bacterial cell by a
bacteriophage
___________________ The process of using mRNA, tRNA, ribosomes and amino acids to
produce a polypeptide
___________________ Small pieces of DNA that can move from one region in a DNA
molecule to another region; occurs rarely
___________________ Small pieces of DNA that are not necessary for the survival of a
bacterial cell, but which often have genes for antibiotic
resistance
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Microbiology Homework #8 Name ________________________

25 points- due ______________ VCC___________

CHAPTER 8- Microbial Genetics; Pathogens

(one point per number unless otherwise stated)

  1. Explain what the difference is between a chromosome and a gene, which is longer?
  2. Due to semiconservative DNA replication, each piece of DNA contains one _____________________ strand of DNA nucleotides and one __________________________ strand of DNA nucleotides.
  3. Define the following words: A. GENOTYPE: B. PHENOTYPE:
  4. Match the following terms: (2 points) Conjugation Transduction Translation Plasmids Transcription Binary Fission Transposons Transformation ___________________ The transfer of DNA from a donor to a recipient bacterial cell as naked pieces of DNA in a solution ___________________ The process of asexual reproduction in bacterial cells ___________________ The process where mRNA is made from a gene ___________________ The transfer of DNA from a donor to a recipient bacterial cell through a sex pilus ___________________ The transfer of DNA from a donor to a recipient bacterial cell by a bacteriophage ___________________ The process of using mRNA, tRNA, ribosomes and amino acids to produce a polypeptide ___________________ Small pieces of DNA that can move from one region in a DNA molecule to another region; occurs rarely ___________________ Small pieces of DNA that are not necessary for the survival of a bacterial cell, but which often have genes for antibiotic resistance
  1. The following is a code for a strand of DNA: (4 points) DRAW HORIZONTAL/VERTICAL LINES TO SEPARATE THE CODE, CODONS, ANTICODONS AND AMINO ACIDS

DNA - A T A T* ___ ___ ___ T T T ___ ___ ___ ___ ___ ___ ___ ___ ___

mRNA A A A C G U U G A tRNA U G G Amino Acid Methionine __________ __________ __________ ___________ Use the information from Figure 8.9 in your textbook and the sequences of mRNA and tRNA made from this DNA seen here to complete the blanks for the segment of DNA shown and fill in the 4 amino acid blanks with the amino acids coded for by this DNA strand. _ATAT = the promoter sequence of DNA that attaches RNA polymerase to the DNA stand._*

  1. Refer to diagram on page 586 The antibiotic chloramphenicol (black pentagon) binds to the 50S ribosomal subunit, blocking protein synthesis. From this information you can conclude that chloramphenicol: a. prevents transcription in eukaryotes b. prevents translation in eukaryotes c. prevents transcription in prokaryotes d. prevents translation in prokaryotes
  2. The anticodon for VALINE is: a. GUU b. CUU c. CTT d. CAA e. GTA
  3. Which one of the following pairs is mismatched? a) DNA polymerase- makes a molecule of DNA from a DNA template b) RNA polymerase- makes a molecule of RNA from a RNA template c) DNA polymerase- needed for DNA replication d) RNA polymerase- needed for protein synthesis e) DNA polymerase- adds DNA nucleotides in a 5’  3’ direction
  4. If a sample of DNA has 16% guanine, what percent of that DNA sample is thymine?
  1. A. What is the problem with seroconversion when dealing with blood transfusions and organ transplants patients? B. Besides Sub-Saharan Africa, what other region of the world has over 5 million cases of HIV/AIDS?
  2. The current treatment for AIDS which is called HAART (highly active antiretroviral therapy) consists of giving patients the following drugs. Explain the reasoning behind how each of the drugs works to inhibit HIV infection: A. reverse transcriptase inhibitors: B. protease inhibitors:
  3. A. What is the difference in the viruses used in the Salk and the Sabin poliomyelitis vaccines? B. Which one can transmit polio to the patient vaccinated with it?
  4. A. What wild animal is the predominant rabies infected animal in Florida? B. Besides animal bites, name 2 other ways that a person could get a rabies infection.
  5. Match the following etiologies with the diseases they cause: A. walking pneumonia _____ Coxiella burnettii B. infectious mononucleosis _____ Streptococcus pneumoniae C. Q fever _____ Epstein-Barr virus D. typical pneumonia _____ Mycoplasma pneumoniae
  6. In 1976 Legionnaire’s Disease was the cause of death for 29 participants in an American Legion Convention in Philadelphia; the source of this respiratory infection was the air conditioners. Name several potential sources of Legionella pneumophila, besides air conditioners, where this microbe could be harbored in YOUR home. _______/