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2025 ALAT Certification Final Exam Latest Questions And Answers Graded A+, Exams of Andragogy

2025 ALAT Certification Final Exam Latest Questions And Answers Graded A 2025 ALAT Certification Final Exam Latest Questions And Answers Graded A 2025 ALAT Certification Final Exam Latest Questions And Answers Graded A

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2024/2025

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2025 ALAT Certification Final Exam Latest Questions And
Answers Graded A
Which of the following species is covered by the Animal Welfare Act?
-Birds bred specifically for research
-Zebrafish
-Mice
-Hamsters - ANSWER- Hamsters
What types of euthanasia methods are listed in the AVMA Guidelines for the
Euthanasia of Animals?
-Suitable, conditionally suitable, and unsuitable methods
-Adequate, acceptable, and inadequate methods
-Acceptable, conditionally acceptable, and unacceptable methods
-Approved, tentative, and unapproved methods - ANSWER- Acceptable,
conditionally acceptable, and unacceptable methods
The PHS Policy covers all live vertebrates used or intended for use in research,
training, experimental purposes and biological testing.
-False
-True - ANSWER- True
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2025 ALAT Certification Final Exam Latest Questions And

Answers Graded A

Which of the following species is covered by the Animal Welfare Act?

  • Birds bred specifically for research
  • Zebrafish
  • Mice
  • Hamsters - ANSWER- Hamsters What types of euthanasia methods are listed in the AVMA Guidelines for the Euthanasia of Animals?
  • Suitable, conditionally suitable, and unsuitable methods
  • Adequate, acceptable, and inadequate methods
  • Acceptable, conditionally acceptable, and unacceptable methods
  • Approved, tentative, and unapproved methods - ANSWER- Acceptable, conditionally acceptable, and unacceptable methods The PHS Policy covers all live vertebrates used or intended for use in research, training, experimental purposes and biological testing.
  • False
  • True - ANSWER- True

Which document on animal research is reviewed and approved by the Institutional Animal Care and Use Committee?

  • Research proposal
  • Grant
  • Animal use protocol
  • Research data report - ANSWER- Animal Use Protocol What federal agency is responsible for enforcing and administering the Animal Welfare Regulations?
  • Animal and Plant Health Inspection Service
  • Food and Drug Administration
  • Office of Laboratory Animal Welfare
  • AAALAC International - ANSWER- Animal and Plant Health Inspection Service What is NOT a requirement of the AWA/AWR a researcher must comply with, if they house covered species?
  • Appoint an unaffiliated member to the IACUC.
  • Submit an annual report including the number of covered species used.
  • Provide dogs with exercise.
  • Meet or exceed sanitization standards for both cages and facility. - ANSWER- Appoint an unaffiliated member to the IACUC.
  1. According to the Animal Welfare Regulations, to which official does the IACUC report?
  • The Institutional Official (IO) or Chief Executive Officer (CEO)
  • The Chair of the Office of Laboratory Animal Welfare or the Area Director
  • The Director of the USDA Animal & Plant Health Inspection Service or Chief Inspecting Officer
  • The Attending Veterinarian or Facility Director - ANSWER- The Institutional Official (IO) or Chief Executive Officer (CEO) Which agency is responsible for enforcing the Public Health Service Policy on Humane Care and Use of Laboratory Animals (PHS Policy)?
  • Food and Drug Administration
  • Animal and Plant Health Inspection Service
  • Institute for Laboratory Animal Research
  • National Institutes of Health Office of Laboratory Animal Welfare - ANSWER- AAALAC International Using the minimum number of animals that will serve a useful purpose, yet yield statistically sound data and produce scientific benefit demonstrates which 3R's principle?
  • Reconstitution
  • Refinement
  • Replacement
  • Reduction - ANSWER- Reduction If an incident of animal misuse is not resolved after contacting your supervisor, you can submit your concerns to whom?
  • Institutional Official
  • IACUC chair
  • CEO
  • AVMA - ANSWER- IACUC Chair What is the common term for the policy that most institutions have to protect an individual who reports instances of animal abuse or neglect?
  • Whistleblower policy
  • Reporting policy
  • Anti-abuse policy
  • Protection policy - ANSWER- Whistleblower policy What federal agency is responsible for enforcing and administering the Animal Welfare Regulations?
  • Animal and Plant Health Inspection Service
  • AAALAC International
  • Office of Laboratory Animal Welfare
  • Food and Drug Administration - ANSWER- Animal and Plant Health Inspection Service In 1967, the Animal Care Panel changed its name to what?
  • American Association for Laboratory Animal Science
  • Association for the Advancement of Laboratory Animal Science
  • Laboratory Animal Science Association
  • American College of Laboratory Animal Science - ANSWER- American Association for Laboratory Animal Science Who led the group of veterinarians that met in Chicago and formed the Animal Care Panel in 1950?
  • Dr. Nathan Brewer
  • Dr. Louis Pasteur
  • Dr. Paul Weiss
  • Dr. Jennifer Smith - ANSWER- Dr. Nathan Brewer What is NOT a method used by researchers to reduce the number of animals used?
  • Using invertebrates rather than mammals
  • Cell culture
  • Computer models
  • Improved statistical methods - ANSWER- Using invertebrates rather than mammals Why are animals used to study human disease?
  • There are no regulations that restrict the use of animals in research.
  • It takes less time to perform an experiment with animals than with humans.
  • The physiological processes in animal models can be very similar to humans.
  • It is less expensive to use animals for research studies. - ANSWER- The physiological processes in animal models can be very similar to humans. Which member of the research team coordinates animal care activities and advises on the selection of appropriate animal models?
  • Research technician
  • Principal investigator
  • Laboratory animal technician
  • Laboratory animal veterinarian - ANSWER- Laboratory animal veterinarian What does the acronym "SOP" stand for?
  • Facility sanitation procedures
  • Recordkeeping
  • Animal husbandry
  • Surgical procedures - ANSWER- Surgical Procedures What mandate or document must be studied in addition to the ALAT Training Manual to prepare for the ALAT technician certification examination?
  • Occupational Health and Safety in the Care and Use of Laboratory Animals
  • AVMA Guidelines for the Euthanasia of Animals and the Animal Welfare Act
  • The Guide for the Care and Use of Laboratory Animals
  • PHS Policy - ANSWER- The Guide for the Care and Use of Laboratory Animals RALAT is the acronym for which of the following?
  • Registered Assistant Laboratory Animal Technician
  • Research Associate Laboratory Animal Technician
  • Registered Associate Laboratory Animal Technician
  • Research Animal Laboratory Assistant Technician - ANSWER- Registered Assistant Laboratory Animal Technician What is the common term for the policy that most institutions have to protect an individual who reports instances of animal abuse or neglect?
  • Whistleblower policy
  • Protection policy
  • Anti-abuse policy
  • Reporting policy - ANSWER- Whistleblower policy Who is ultimately responsible for the safety of an individual staff member of an animal research facility?
  • The individual him/herself
  • The individual's supervisor
  • Senior management
  • The institutional official - ANSWER- The individual him/herself Who should receive training for handling security issues?
  • Only environmental health and safety staff
  • Supervisors and other management only
  • Just members of the emergency response team
  • Everyone in the animal care and use program - ANSWER- Everyone in the animal care and use program What is the best way to measure the effectiveness of an institution-wide emergency system?
  • Responding to a real emergency
  • Comparing the system with that in similar institutions
  • Periodic disaster drills
  • Statistical analysis - ANSWER- Periodic disaster drills What is the main reason that post-incident discussions are useful?
  • New safety officials can be chosen during the discussions.
  • They can be used to identify ways to improve emergency procedures.
  • They can be used to train individuals who did not follow the proper procedures.
  • The discussions help with planning the next disaster drill. - ANSWER- They can be used to identify ways to improve emergency procedures. Which of the following is NOT a potential consequence of the "liberation" of research animals?
  • Destruction of the research program
  • Death of the animals
  • Loss of IACUC approval
  • Theft or loss of dangerous infectious agents - ANSWER- Loss of IACUC Approval Why is it important that a background check is done for any potential new employees of an animal research facility?
  • To avoid hiring anyone with poor credit
  • To avoid hiring anyone who has not lived in the state for at least a year
  • To avoid hiring anyone opposed to the use of animals in research
  • To avoid hiring anyone who has been involved in a legal suit during the last year
  • ANSWER- To avoid hiring anyone opposed to the use of animals in research

An exercise which involves the mock destruction of part of the facility due to an earthquake is an example of:

  • a security plan.
  • an act of vandalism.
  • a drill.
  • a security breach. - ANSWER- a drill If infectious agents are used in the facility, it is subject to standards outlined by which regulatory agency?
  • The Office of Laboratory Animal Welfare
  • The Animal and Plant Health Inspection Service
  • The Centers for Disease Control
  • The Nuclear Regulatory Commission - ANSWER- The Centers for Disease Control Only individuals with existing allergies are likely to develop allergies to animals. True False - ANSWER- False Why should personnel always wash their hands BEFORE entering an animal facility?
  • Animals are sensitive to the smell of food and are more likely to bite.
  • Surfaces in the vivarium are potential sources of contamination.
  • To prevent transferring pathogens to other staff.
  • Animals in the facility should not be exposed to microorganisms from staff members' pets. - ANSWER- Animals in the facility should not be exposed to microorganisms from staff members' pets. Which statement about personal protective equipment is TRUE?
  • Face shields offer the best protection for the eyes from splashes and aerosols.
  • Puncture resistant gloves do not guarantee protection from injury but provide additional protection should handling or restraint techniques fail.
  • Masks are mandatory in all areas of the vivarium.
  • Cloth uniforms should be taken home and laundered at least every week. - ANSWER- Puncture resistant gloves do not guarantee protection from injury but provide additional protection should handling or restraint techniques fail. How should pressurized gas cylinders be stored?
  • Flat on the floor, in a cool dry place
  • Chained down flat to prevent movement
  • Upright, in a small enclosed space
  • Secured to a wall - ANSWER- Secured to a wall Which document provides all the information about a chemical substance used in the facility?
  • Animal use protocol
  • The GLPs
  • Safety data sheet
  • Biosafety in Microbiological and Biomedical Laboratories (BMBL) - ANSWER- Safety Data Sheet Which of the following statements regarding radioactive materials is TRUE?
  • Your distance from a radioactive substance has NO impact on your radiation exposure.
  • No special precautions need to be taken cleaning the cages of animals dosed with radioactivity
  • Radiation dosage is cumulative and is monitored with film badges.
  • All forms of radiation can pass through the body and cause physical damage - ANSWER- Radiation dosage is cumulative and is monitored with film badges. Shielding, time, and distance are the key factors for personal protection against which of the following?
  • Mechanical injuries
  • Radiation
  • Allergies
  • Injuries from bites and scratches - ANSWER- Radiation Whose standards specifically address the storage and handling of radioactive materials in the facility?
  • Wearing gloves, bonnet, gown, and shoe covers when in animal rooms - ANSWER- Keeping a snack in the pocket of your scrubs and eating it in
  • Applying makeup and eating lunch in the breakroom.
  • Keeping a snack in the pocket of your scrubs and eating it in between cage changes
  • Washing hands before leaving animal areas between cage changes What is the term for a disease that can be transmitted between animals and humans? -Plasmosis
  • Infectious hazard
  • Zoonosis
  • Epidemic - ANSWER- Zoonosis What is the minimum sound level that is considered a noise hazard?
  • 85 dB and above
  • 50 dB and above
  • 100 dB and above
  • 125 dB and above - ANSWER- 85 dB and above What should be the first step if a technician is bitten by an animal in the animal facility?
  • The wound should be scrubbed and held under running water for 15 minutes.
  • The technician should stop all activities and notify their supervisor.
  • The wound should have antibiotic ointment applied, then held under running water for 15 minutes.
  • Pressure should be applied to the wound, then a cold compress. - ANSWER- The wound should be scrubbed and held under running water for 15 minutes. Once sanitized, caging and equipment is best stored in which location?
  • In the clean side of the cage wash area
  • In the hallways leading to the cage wash area
  • In the dirty side of the cage wash area
  • In the hallways just outside of the animal rooms - ANSWER- In the clean side of the cage wash area Foamers aerate what two ingredients into a sanitizing foam?
  • Water and disinfectant
  • Rubbing alcohol and detergent
  • Nitrous oxide and iodine
  • Carbon dioxide and soap - ANSWER- Water and disinfectant Which type of sanitization equipment can decontaminate a room using a disinfectant without manually washing or scrubbing surfaces?
  • Cabinet washers and bottle washers
  • Misters and foggers
  • Rack washers and tunnel washers
  • Pressure washers and foamers - ANSWER- Misters and foggers Cages may be washed along with the rack when using a rack washer True False - ANSWER- true To reduce the numbers of pathogenic microorganisms, animal drinking water may be treated with which of the following? Select all that apply.
  • Water softener
  • Gamma radiation
  • Alkali
  • Reverse osmosis
  • UV radiation
  • Chlorine
  • Acid - ANSWER- Reverse Osmosis, UV Radiation, Chlorine, Acid In reference to change stations or laminar flow cabinets, the abbreviation "HEPA", as in a HEPA filter, refers to:
  • humidity elevated partial airflow.
  • high efficiency particulate air.
  • heat-enriched pathogen absorption.
  • There was too much pressure inside the autoclave.
  • The equipment malfunctioned.
  • Sterilization was not achieved. - ANSWER- Sterilization was not achieved. What are tissue digesters used for?
  • Diet preparation
  • Wound care
  • Cold and flu prevention
  • Carcass disposal - ANSWER- carcass disposal Why should a vacuum in an animal facility have a HEPA filter?
  • It enhances the suction needed for cleaning.
  • It minimizes the spread of dust and allergens.
  • It creates less noise when vacuuming.
  • It lowers the turbulence in airflow. - ANSWER- It minimizes the spread of dust and allergens. How often should scales be inspected and calibrated by a certified scale technician?
  • Each time an animal who appears to be obese is weighed.
  • Quarterly
  • Annually
  • After every use - ANSWER- annually Safety features on cage washers, such as a door release mechanism, are designed to prevent accidental entrapment of personnel because:
  • water gets deep during cage wash cycles and can cause drowning.
  • the lack of oxygen inside the cage washer is very dangerous.
  • the high temperatures used in cage wash cycles can be lethal.
  • the recycled water used in cage washers may be unhealthy. - ANSWER- the high temperatures used in cage wash cycles can be lethal. Chemical fume hoods do not protect:
  • personnel from exposure to volatile gases.
  • personnel from exposure to allergens.
  • animals from exposure to pathogens in the air.
  • animals from exposure to volatile gases. - ANSWER- animals from exposure to pathogens in the air. What type of feeder is commonly used for pelleted rabbit and guinea pig feeds?
  • Feed bowl on the floor of the cage
  • V-shaped feeder built into cage lid
  • Suspended bowl attached to the cage or pen wall
  • J-shaped clip-on feeder - ANSWER- J-shaped clip-on feeder Which of the following statements about biological cabinet safety features is TRUE?
  • Biological safety cabinets require yearly certification to ensure that they are working properly.
  • All biological safety cabinets are airtight.
  • Biological safety cabinets do not need HEPA filters because they are airtight.
  • Biological safety cabinets are ductless to prevent microorganisms from getting into the air. - ANSWER- Biological safety cabinets require yearly certification to ensure that they are working properly. Which biosafety cabinets are known as "glove boxes"?
  • Class III
  • Class II, Type B
  • Class II A
  • Class I - ANSWER- Class III Large ovens that burn tissues, trash, and other materials to ash are called:
  • garbage stoves.
  • incinerators.
  • refuse ovens.
  • industrial ranges. - ANSWER- Incinerators What is the advantage of using an autoclave over other sterilization methods?
  • It uses inexpensive chemicals, which makes it inexpensive to use.
  • It is safe and requires no personal protective equipment to use.
  • This type of plastic is opaque, and it is harder to observe the animals. - ANSWER- This type of plastic is opaque, and it is harder to observe the animals. Which statement is TRUE about standard individually ventilated cages?
  • They cannot be used for biocontainment studies.
  • Bottled water cannot be used with individually ventilated caging systems.
  • They encourage an exchange of microbes between cages, so extra precautions must be taken.
  • If the ventilation fails, carbon dioxide levels can rise inside the cages. - ANSWER- If the ventilation fails, carbon dioxide levels can rise inside the cages. Why are cage clamps required on biocontainment cages?
  • To ensure a negative pressure environment
  • To prevent accidental opening of the cage
  • To provide a means to lift the cage
  • To exert pressure on the cage top to maintain filtration - ANSWER- To prevent accidental opening of the cage The Guide for the Care and Use of Laboratory Animals does not recommend wire- bottomed caging for rodents because of the potential for:
  • cross-contamination.
  • increased soiling with feces.
  • hypothermia.
  • foot injuries. - ANSWER- foot injuries Appropriate caging should balance the needs of the animal with:
  • the ability to store cages properly in the facility.
  • the odors generated in the room.
  • the ability to provide for sanitation.
  • the needs of the animal care staff. - ANSWER- the ability to provide for sanitation. Aquatic caging must have which two features?
  • Cage clamps and a drain
  • High sides and resting platforms
  • A water source and a drain
  • Overflow valves and cage clamps - ANSWER- A water source and a drain Which of the following statements about the size of animal caging is FALSE?
  • An animal must have at least enough room to stand up, lie down, and turn around.
  • The size of the floor space includes areas occupied by food and water containers.
  • The Guide and the AWR describe minimum cage sizes for most species.
  • The size of the cage is usually based on the animal's body weight, and other parameters - ANSWER- The size of the floor space includes areas occupied by food and water containers. Appropriate caging should balance the needs of the animal with:
  • the odors generated in the room.
  • the needs of the animal care staff.
  • the ability to store cages properly in the facility
  • the ability to provide for sanitation. - ANSWER- the ability to provide for sanitation. Which of the following is NOT a characteristic of acceptable caging?
  • Encourages breeding
  • Secure against escape
  • Allows for normal physiological needs
  • Adequately ventilated - ANSWER- Encourages breeding What type of caging is the term "jump box" used to describe?
  • Activity cage
  • Exercise cage
  • Transport cage
  • Shoebox cage - ANSWER- --transport cage What is an advantage of using stainless steel in caging?
  • It is less expensive than other metals.
  • It does not have to be sanitized as often.
  • It does not rust or corrode.
  • It is lighter weight than aluminum. - ANSWER- --does not rust or corrode