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6 Questions on Molecular Genetics - Exam 2 - Fall 2007 | BI 381, Exams of Molecular biology

Material Type: Exam; Professor: Gathman; Class: Molecular Genetics; Subject: Biology; University: Southeast Missouri State University; Term: Spring 2007;

Typology: Exams

Pre 2010

Uploaded on 08/08/2009

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BI 381 Exam 2 Spring 2007
Instructions: 1. Relax. 2. Genetic code and wobble tables at the end of the exam. 3. Look
at all the questions and do the ones you know best first.
1. a. (10 points) Explain how intrinsic transcription termination occurs in a prokaryote.
b. (10 points) Explain how abortive initiation of transcription occurs in a prokaryote.
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BI 381 Exam 2 Spring 2007 Instructions: 1. Relax. 2. Genetic code and wobble tables at the end of the exam. 3. Look at all the questions and do the ones you know best first.

  1. a. (10 points) Explain how intrinsic transcription termination occurs in a prokaryote. b. (10 points) Explain how abortive initiation of transcription occurs in a prokaryote.
  1. Figures 1c and 2b from Kozmin et al. are shown below. Stars mark groups of genotypes that were significantly different; fig. 1c shows three groups that are different from each other, and fig. 2b shows two groups that are different from each other. The functions of the wild type alleles of the genes referred to here are as follows: OGG codes for 8- oxoguanine N-glycosylase, RAD14 codes for lesion-binding protein required for yeast NER, and PHR1 codes for photolyase. ber- indicates a strain with mutant alleles of several genes encoding N-glycosylases, including ogg. Fig 1c. Fig. 2b. Questions on the next page refer to these graphs.

3.a. (10 points) Describe the process of intron removal and exon splicing in a eukaryotic RNA transcript. Be as specific as possible about the snurps involved and the sequence of events. b. (5 points) Describe the process of forming the meG cap on a eukaryotic RNA transcript, being as specific as possible about the enzymes involved and the sequence of events.

4 a. (15 points) In the table below, indicate whether each mutant organism will be able to grow when placed on each medium by putting + for growth of the organism and - for no growth of the organism. Please make them clear so I can read them. Notes: Compound B is hydrolyzed to yield E and F; compounds F and D are combined by condensation to form G; compounds G and H are combined by condensation to form I. The organism must either make or receive compound I in order to grow. Each mutant is mutant for only one gene (coding for one enzyme). Minimal Medium Supplemented with Compound Mutant (None) A B C D E F G H I 1 2 3 4 5 6 7 8 b. (5 points) If a colony of mutant 4 cells is placed on minimal medium, what compound(s) will accumulate inside the mutant cells? Just answer, don't explain. c. (5 points) Suppose you had a double mutant strain of this organism, containing the mutant alleles from both mutants 2 and 7. Would it grow on any of the supplemented media listed above? If so, which one(s)? Just answer, don't explain.