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Microbiology and Antimicrobial Agents: Terminology and Mechanisms - Prof. King-Thom Chung, Study notes of Microbiology

This description provides definitions and descriptions of various terms related to microbiology and antimicrobial agents. It covers topics such as aerosols, antiseptics, autoclaves, disinfectants, filtration, pasteurization, sterilization, and more. It also includes specific agents like antibiotics, drug resistance, and spectrum of activity.

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2011/2012

Uploaded on 04/25/2012

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Chapter 12-13 terminology check:
Aerosol – droplets released into the air
Antiseptic – agents applied to living tissue
Autoclave – increases temperature and pressure in order to clean. Temp goes up to 121 C. This raise in
temp is high enough to kill spores, and vegetative arganisms as well as disrupt the structure of nucleis
acids in viruses.
Bactericidal- killing
Bacteriostatic- growth inhibiting
Cavitation- formation of a partial vacuum in a liquid
decimal reduction time (DRT or D value) – the length of time needed to kill 90% of the organism in a
given population at a specified temp.
Disinfectant- agents applied to inanimate objects
Disinfection –reducing the number of pathogenic organisms on objects, or in materials so they pose no
threat of disese.
Paper filter method- uses small filter paper disks each soaked with a different chemical agent placed on
an agar plate that has been inoculated with a test organism.
Filtration- is the passage of material through a filtration device, sterilization through filtration requires a
filter with exceedingly small pores.
Lyophilization – is the drying of material from the frozen state
Pasteurization- a process invented by Pasteur to destroy organisms that caused wine to sour. Does not
achieve sterility but does kill pathogens especially mycobacterium and salmonella.
Phenol coefficient- (page 241) since Lister introduced phenol (carbolic acid) in 1867, it has been the
standard disinfectant to which other disinfectants are compared to under the same conditions.
Plasmolysis- loss of water, interferes with cell function.
quaternary ammonium compounds (quats page 345)- cationic detergents, which have four organic
groups attatched to a nitrogen atom. A variety of quats are available as disinfectants their chemical
structure vary according to their organic groups.
Rad- a unit of radiation energy absorbed per gram of tissue
Sonication-the disruption of cells by sounds waves
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Chapter 12-13 terminology check: Aerosol – droplets released into the air Antiseptic – agents applied to living tissue Autoclave – increases temperature and pressure in order to clean. Temp goes up to 121 C. This raise in temp is high enough to kill spores, and vegetative arganisms as well as disrupt the structure of nucleis acids in viruses. Bactericidal- killing Bacteriostatic- growth inhibiting Cavitation- formation of a partial vacuum in a liquid decimal reduction time (DRT or D value) – the length of time needed to kill 90% of the organism in a given population at a specified temp. Disinfectant- agents applied to inanimate objects Disinfection –reducing the number of pathogenic organisms on objects, or in materials so they pose no threat of disese. Paper filter method- uses small filter paper disks each soaked with a different chemical agent placed on an agar plate that has been inoculated with a test organism. Filtration- is the passage of material through a filtration device, sterilization through filtration requires a filter with exceedingly small pores. Lyophilization – is the drying of material from the frozen state Pasteurization- a process invented by Pasteur to destroy organisms that caused wine to sour. Does not achieve sterility but does kill pathogens especially mycobacterium and salmonella. Phenol coefficient- ( page 241 ) since Lister introduced phenol (carbolic acid) in 1867, it has been the standard disinfectant to which other disinfectants are compared to under the same conditions. Plasmolysis- loss of water, interferes with cell function. quaternary ammonium compounds (quats page 345 )- cationic detergents, which have four organic groups attatched to a nitrogen atom. A variety of quats are available as disinfectants their chemical structure vary according to their organic groups. Rad- a unit of radiation energy absorbed per gram of tissue Sonication-the disruption of cells by sounds waves

Sterility-no living organisms in or on material. There are no degrees of sterility. Sterilization- the killing or removal of all microorganisms on an object or in a material. Surfactant- soluble compounds that reduce surface tension, just as soaps and detergents break up grease. Thermal death point- the temperature that kills all the bacteria in a 24hr broth culture at neutral Ph in ten minutes. Thermal death time-the time required to kill all the bacteria in a particular culture in a specified time at a specified temp. Tincture-page Ultrahigh temperature processing (UHT)-raises the temp from 74C to 140C and back to 74C in less than 5 seconds. Use-dilution test-uses standard preparations of certain test bacteria. Wetting agent- often used with other chemical agents to help the agent penetrate fatty substances. Amantadine- trycyclic amine, prevents influenza A viruses from penetrating cells Aminoglycoside – are obtained from various species of the genera Streptomyces and Micromonospora. Antagonism –when some drugs are less affective when used in combination than when used alone page 374 Antibiosis- against life Antibiotic- A chemical substance produced by microorganisms which has the capacity to inhibit the growth of bacteria and even destroy and other microorganisms in dilute solution. Antimetabolite- substances that affect the utilization of metabolites and therefore prevent a cell from carrying out necessary metabolic reactions. Antimicrobial agent-a special group of chemotherapeutic agents used to treat disease caused by microbes. Broad spectrum-agents affective against a great number of micro-organisms Carbapenem-represent a new group of bactericidal antibiotics with two part structures. Cephalosporin-neutral, derived from several species of the fungus Cephalosporium and have limited antimicrobial action Chemotherapeutic agent-Any chemical substance used in medical practices, also refered to as drugs.

Minimum bacteriocidal concentration (MBC) –the lowest concentrartion of a chemotherapeutic agent that yields no growths following the second inoculation. Minimum inhibitoy concentration (MIC) – the lowest concentration of the agent that prevents visible growth. Molecular mimicry – actions of antimetabolites which mimic the normal molecule Narrow spectrum-are affective against a small number of micro organisms. Niclosamide –interferes with carbohydrate metabolism, thereby causing a parasite to release large quantities of latic acid. Nitrofuran – are antibacterial drugs that enter susceptible cells and apparently damage sensitive microbial respiratory systems. Penicillin – page 378 Polyene - a family of antibiotics that consists of antifungal agents that contain at least two double bonds Polymyxin- “triple antibiotic” topical Primaquine – interferes with protein synthesis especially in red blood cells. Quinine- used to treat malaria, one of the first chemotherapeutic agents. Quinolone- a new group of synthetic analogs of nalidixic acid, are effective against many Gram+ and Gram- bacteria. Its mode of action is to inhibit bacterial DNA synthesis…. Page 383 Resistance- a microorganism formerly susceptible to the action of antibiotics is no longer affected by it. Resistance R plasmid- each R plasmid carry as many as six or seven genes, each of which confers resistance to a different antibiotic. These canbe transferred from one bacteria to another. R factor-page 372 Rifamycin- bactericidal, blocks RNA transcription. Selective toxicity- must harm the microbes without causing significant damage to the host. semisynthetic drug- antimicrobial agent made by both laboratory synthesis and microorganisms serum- blood plasma, minus the clotting factors serum killing power- page 376 spectrum of activity- the range of different microbes against which an antimicrobial agent acts.

Streptomycin- page 381 Sulfonamide- a large group of bacteriostatic agents. Act by blocking the synthesis of folic acid which is needed to make the nitrogenous bases of DNA. Superinfection- difficult to treat because they are susceptible to few antibiotics. Caused by ivasion by replacement microflora. Synergism- how two drugs work together to kills a bacteria etc page 374 Synthetic drug- agents synthesized in a lab. Tetracycline- bacteriostatic , easily enter the cytoplasm of host cells. Has the widest spectrum of any antibiotics but can destroy normal flora of the intestinal tract leading to gastrointestinal disorders. therapeutic dosage level- level at which the pathogenic organisms are fully eliminated. Toxic dosage level- the level which causes the host damage Zone of inhibition- clear areas