NAME __________________________________ Mutations Worksheet
Deletion, Insertion & Substitution
There are several types of mutation:
DELETION (a base is lost/deleted)
INSERTION (an extra base is added/inserted)
--- Deletion & insertion may cause what’s called a FRAMESHIFT mutation, meaning the reading “frame" changes, thus changing the
amino acid sequence from this point forward
SUBSTITUTION (one base is substituted for another)
--- If a substitution changes the amino acid, it’s called a MISSENSE mutation
--- If a substitution does not change the amino acid, it’s called a SILENT mutation
--- If a substitution changes the amino acid to a “stop,” it’s called a NONSENSE mutation
Complete the boxes below. Classify each as Deletion, Insertion or Substitution AND as either
frameshift, missense, silent or nonsense (Hint: Deletion & Insertion will always be frameshift).
Original DNA Sequence:T A C A C C T T G G C G A C G A C T …
mRNA Sequence:
Amino Acid Sequence:
Mutated DNA Sequence #1 T A C A T C T T G G C G A C G A C T …
What’s the mRNA sequence? (Circle the change)
amino acid sequence?
Will there likely be effects? What type of mutation is this? ________________________________
Mutated DNA Sequence #2 T A C G A C C T T G G C G A C G A C T …
What’s the mRNA sequence? (Circle the change)
amino acid sequence?
Will there likely be effects? What type of mutation is this? ________________________________
Mutated DNA Sequence #3 T A C A C C T T A G C G A C G A C T …
What’s the mRNA sequence? (Circle the change)
amino acid sequence?
Will there likely be effects? What type of mutation is this? ________________________________
Mutated DNA Sequence #4 T A C A C C T T G G C G A C T A C T …
What’s the mRNA sequence? (Circle the change)
amino acid sequence?
Will there likely be effects? What type of mutation is this? _________________________________
