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๏ท ASCP-Chemistry Section Exam | Latest 2025 \ 2026
With 100% Correct Answers
Aldosterone is released by the adrenal cortex upon stimulation by:
A. Renin
B. Angiotensinogen
C. Angiotenisinogen I
D. Angiotensinogen II - โโD. Angiotensinogen II
Clinical Assays for tumor markers are most important for:
A. screening for the presence of cancer
B. monitoring the course of known cancer
C. confirming the absence of disease
D. identifying patients at risk for cancer - โโB. Monitoring the course of known cancer
Detection of which of the following substances is most useful to monitor the course of a patient with testicular cancer?
A. alpha-fetoprotein
B. carcinoembryonic antigen
C. prolactin
D. testosterone - โโA. alpha-fetoprotein
Increased concentrations of alpha-fetoprotein (AFP) in adults are most characteristically associated with:
A. hepatocellular carcinoma
B. alcoholic cirrhosis
C. chronic active hepatitis
D. multiple myeloma - โโA. hepatocellular carcinoma
Carcinoembryonic antigen (CEA) is most likely to be produced in a maignancy involving the:
A. Brain
B. Testes
C. Bone
D. Colon - โโD. Colon
Which of the following is useful in the detection an management of carcinoma of the prostate?
A. total prostate-specific antigen
B. prostatic acid phosphatase
C. human chorionic gonadotropin
D. alpha-fetoprotein - โโA. total prostate-specific antigen
A drug has a half-life of 6 hours. If a dose is given every 6 hours, a steady-state drug level would usually be achieved in:
A. 3-5 hours
B. 10-12 hours
C. 24-42 hours
D. 48-50 hours - โโC. 24-42 hours
The drug procainamide is prescribed to treat cardiac arrhythmia. What biologically active liver metabolite of procainamide is often measured simultaneously?
A. phenobarbitol
B. quinidine
C. N-acetyl procainamide (NAPA)
D. lidocaine - โโC. N-acetyl procainamide (NAPA)
Cocaine is metabolized to:
A. carbamazepine
B. codeine
C. hydrocodone
D. benzolecgonine - โโD. benzolecgonine
The metabolite 11-nor-tetrahydrocannabinol-9-COOH can be detected by immunoassay 3-5 days after a single use of:
A. methamphetamine
B. ccaine
C. benzodiazepine
D marijuana - โโD. marijuana
A 3-yr-old child was evaluated for abdominal pain and anorexia by a physician. A CBC revealed a hemoglobin of 9.8 g/dL (98 g/L) and basophilic stippling of the RBC's. The doctor should order further tests to check for poisoning from:
A. arsenic
B. iron
C. mercury
D. lead - โโD. lead
A carbonate salt used to control manic-depression disorders is:
A. digoxin
B. acetaminophen
C. lithium
D. phenytoin - โโA. digoxin
A salicylate level is performed to detect toxicity caused by ingestion of excess:
A. acetaminophen
B. aspirin
C. ibuprofen
D. psuedoephedrine - โโB. aspirin
Lithium therapy is widely used in the treatment of:
A. hypertension
B. hyperactivity
C. aggression
D. manic-depressive (bipolar) disorder - โโD. manic-depressive (bipolar) disorder
Testing for the diagnosis of lead poisoning should include:
A. erythrocyte protoporphyrin (EPP)
B. urine delta-aminolevulinic acid
C. whole blood lead
D. zinc protoporphyrin (ZPP) - โโC. whole blood lead
Blood received in the laboratory for blood gas analysis must meet which of the following requirements?
A. on ice, thin fibrin strands only, no air bubbles
B. on ice, no clots, fewer than 4 air bubbles
C. on ice, no clots, no air bubbles
D. room temperature, no clots, no air bubbles - โโC. on ice, no clots, no air bubbles
In a specimen collected for plasma glucose analysis, sodium fluoride:
A. serves as a coenzyme of hexokinase
B. prevents reactivity of non-glucose reducing substances
C. precipitates proteins
D. inhibits glycolysis - โโD. inhibits glycolysis
As part of a hyperlipidemia screening program, the following results were obtained on a a 25-yr- old woman 6 hours after eating:
triglycerides: 260 mg/dL (2.86 mmol/L)
cholesterol: 120 mg/dL (3.12 mmol/L)
Which is the best interpretation of these results?
A. both results are normal and not affected by the recent meal
A. cholesterol
B. triglyceride
C. creatinine
D. glucose - โโD. glucose
An arterial blood specimen submitted for blood gas analysis was obtained at 8.30am but was not received in the laboratory until 11am. The technologist should:
A. perform test immediately upon receipt
B. perform test only if specimen was submitted in ice water
C. request a venous blood specimen
D. request a new arterial specimen be obtained - โโD. request a new arterial specimen be obtained
A 24-hour urine specimen (total volume=1,136 mL) is submitted to the laboratory for quantitative urine protein. Calculate the amount of protein excreted per day, if the total protein is 52 mg/dL.
A. 591 mg
B. 487 mg
C. 220 mg
D. 282 mg - โโA 591 mg
The following results were obtained:
urine creatinine: 90 mg/dL
serum creatinine: 0.90 mg/dL
patient's total body surface: 1.73 m
total urine volume in 24 hours: 1500 mL
Given the above data, the patient's creatinine clearance, in mL/min, is:
A. 104
B. 124
C. 144
D. 150 - โโA. 104 mL/min
Which of the following is the formula for calculating absorbance given the percent transmittance (%T) of a solution?
A. 1-log(%T)
B. (log(%T))/
C. 2 x log(%T)
D. 2-log(%T) - โโD. 2-log(%T)
Which of the following is the Henderson-Hasselbach equation?
A. indirect ion-selective electrodes
B. direct ion-selective electrodes
C. spectrophotometric
D. fluorescence - โโA. indirect ion-selective electrodes
To detect barbituate abuse when analyzing urine specimens, immunoassay os the method of choice for screening. The method of choice for confirmation is:
A. nephelometry
B. thin-layer chromatography
C. gas chromatography/mass spectrometry
D. ultraviolet absorption spectroscopy - โโC. gas chromatography/mass spectrometry
When separating serum protein by cellulose acetate electrophoresis, using Veronal buffer at pH 8.6, beta globulin migrates:
A. faster than albumin
B. slower than gamma globulin
C. faster than gamma globulin
D. faster than alpha-2 globulin - โโC. faster than gamma globulin
Hemoglobin S can be separated from hemoglobin D by which of the following methods?
A. agar gel electrophoresis at pH 5.
B. thin-layer chromatography
C. alkali denaturation
D. ammonium precipitation - โโA. agar gel electrophoresis at pH 5.
What is the proper pH for the buffered solution used to perform serum protein electrophoresis?
A. 5.
B. 7.
C. 8.
D. 9.6 - โโC. 8.
The buffer pH most effective at allowing amphoteric proteins to migrate toward the cathode in an electrophoretic system would be:
A. 4.
B. 7.
C. 8.
D. 9.5 - โโA. 4.
Coulometry is often used to measure:
A. chloride in sweat
B. the pH in saliva
C. bicarbonate in urine
D. ammonia in plasma - โโA. chloride in sweat
In a pH meter reference electrodes may include:
A. silver-silver chloride
B. quinhydrone
C. hydroxide
D. hydrogen - โโA. silver-silver chloride
Most automated blood gas analyzers directly measure:
A. pH, HCO3, and % O2 saturation
B. pH, PCO2, and PO
C. HCO3, PCO2, and PO
D. pH, PO2, and % O2 saturation - โโB. pH, PCO2, and PO
Blood PCO2 may be measured by:
A. direct colorimetric measurement of dissolved CO
B. a self-contained potentiometric electrode
C. measurement of CO2-saturated hemoglobin
D. measurement of CO2 consumed at the cathode - โโB. a self-contained potentiometric electrode
Valinomycin enhances the selectivity of the electrode used to quantitate:
A. sodium
B. chloride
C. potassium
D. calcium - โโC. Potassium
Which blood gas electrode is composed of silver/silver chloride reference electrode and glass?
A. PO
B. pH
C. PCO
D. HCO3 - โโC. PCO
Most chemical methods for determining total protein utilize which of the following reactions?
A. hexokinase
B. glucose oxidase
C. glucose-6-phosphatase
D. glucose dehydrogenase - โโA. hexokinase
Which of the following calcium procedures utilizes lanthanum chloride to eliminate interfering substances?
A. o-cresolphthalein complexone
B. precipitation with chloranilic acid
C. chelation with EDTA
D. atomic absorption spectrophotometry - โโD. atomic absorption specttrophotometry
Before unconjugated bilirubin can react with Ehrlich diazo reagent, which of the following must be added?
A. acetone
B. ether
C. distilled water
D. caffeine - โโD. caffeine
The most widely used methods for bilirubin measurement are those based on the:
A. Jaffe reaction
B. Schales and Schales method
C. 8-hydroxyquinoline reaction
D. Jendrassik-Grof method - โโD. Jendrassik-Grof method
In the Malloy and Evelyn method for the determination of bilirubin, the reagent that is reacted with bilirubin t form a purple azobilirubin is:
A. dilute sulfuric acid
B. diazonium sulfate
C. sulfobromophthalein
D. diazotized sulfanilic acid - โโD. diazotized sulfanilic acid
In the Jendrassik-Grof reaction for total bilirubin, bilirubin reacts wth diazotized sulfanilic acid to form:
A. diazo bilirubin
B. biliverdin
C. azobilirubin
D. bilirubin glucurnide - โโA. diazo bilirubin
In the assay of lactate dehydrogenase, which of the following products if actually measured?
