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Bacterial Growth Lab and Worksheet, Exercises of Bacteriology

Typology: Exercises

2020/2021

Uploaded on 04/20/2021

arlie
arlie 🇺🇸

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Name: Date:
Bacterial growth
How effective is washing your hands or
using hand sanitizer on removing bacteria? Let’s find out!
We will be taking samples of the bacteria on the surface of our own hands and watching it
grow! Samp les will be taken from one student’s hand under three different conditions:
1. Unwashed hand
2. Hand washed w ith soap and water
3. Hand sanitized with antibacterial hand gel
Use this worksheet to collect and organize your data, and remember to keep it handy since this
experiment will continue for one week!
Streak your plates
1. Chose
one
student from your group to obtain samples from (it is important to have all
samples come from the same person to reduce experimental error!).
2. Label 3 Petri dishes, writing your group number, sample name (unwashed, washed, or
sanitized), and class on the lid with a Sharpie.
3. Using a Q-tip, rub the Q-tip on the surface of the student’s palm. Open your Petri dish
(labeled “unwashed”) containing agar and rub the Q-tip back and forth on the agar. B e
careful not to apply too much pressure when doing this, otherwise the agar will tear.
4. Close the Petri dish.
5. Wash
one
hand with soap and water (may need assistance from a teacher or group
member) and repeat Steps 3 and 4 , except being careful to streak the plate labeled
“washed” this time.
6. Apply hand sanitizer to
the other
hand (may need assistance from a teacher or group
member) and let it air dry. Repeat Steps 3 and 4 , except being careful to streak the plate
labeled “sanitized” this time.
pf3

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Download Bacterial Growth Lab and Worksheet and more Exercises Bacteriology in PDF only on Docsity!

Name: Date:

Bacterial growth

How effective is washing your hands or using hand sanitizer on remo ving bacteria? Let’s find out!

We will be taking samples of the bacteria on the surface of our own hands and watching it grow! Samples will be taken from one student’s hand under three different conditions:

  1. Unwashed hand
  2. Hand washed with soap and water
  3. Hand sanitized with antibacterial hand gel

Use this worksheet to collect and organize your data, and remember to keep it handy since this experiment will continue for one week!

Streak your plates

  1. Choseone student from your group to obtain samples from (it is important to have all samples come from the same person to reduce experimental error!).
  2. Label 3 Petri dishes, writing your group number, sample name (unwashed, washed, or sanitized), and class on the lid with a Sharpie.
  3. Using a Q-tip, rub the Q-tip on the surface of the student’s palm. Open your Petri dish (labeled “unwashed”) containing agar and rub the Q-tip back and forth on the agar. Be careful not to apply too much pressure when doing this, otherwise the agar will tear.
  4. Close the Petri dish.
  5. Washone hand with soap and water (may need assistance from a teacher or group member) and repeat Steps 3 and 4, except being careful to streak the plate labeled “washed” this time.
  6. Apply hand sanitizer tothe other hand (may need assistance from a teacher or group member) and let it air dry. Repeat Steps 3 and 4, except being careful to streak the plate labeled “sanitized” this time.

Table 1: Fill in this table with after analyzing the images of your Petri dish with the area covered by the bacteria (in centimeters squared). Also record any comments or observations on the sample during the experiment.

Sample

Area covered by bacteria (cm^2 Comments & observations

Day 0 Day 4 Day 7

Unwashed hand

Washed hand

Sanitized hand

Plot the area covered by bacteria of the 3 samples versus time (in days) below. Make one line for each sample and chose a different color for each line. Make a legend to label which color corresponds with which sample.

Figure 1 : Plot of bacterial growth over time for unwashed hand, hand washed with soap and water, and hand sanitized with antibacterial hand gel.

0

5

10

15

20

25

30

35

40

45

50

0 1 2 3 4 5 6 7 8

Area of Petri dish covered by bacteria (cm

Time (days)

Bacterial growth over time