Download Microbiology Exam Questions and Detailed Solutions and more Exams Biology in PDF only on Docsity!
BIO|| 331 || Exam|| 2 || with|| precise || detailed ||
solutions
EN1 || quiz- || what || nitrogen || compound || is || used || as || a || substrate || for || denitrification? ||
- || Correct || answer || ✔nitrate || (NO3-) EN1 || quiz- || for || what || metabolic || purpose || is || nitrate || used || in || denitrification? || - || Correct || answer || ✔electron || acceptor || for || anaerobic || respiration EN1 || quiz- || what || is || the || waste || product || of || denitrification || - || Correct || answer || ✔nitrogen || (n2) P1&P3 || quiz- || what || is || the || targeted || % || survival || for || mutagenesis || - || Correct || answer || ✔.1-1% P1&P3 || quiz- || for || UV || mutagenesis || of || Serratia, || order || steps || of || procedure || from || beginning || to || end UV || exposure Plate || on || PG || medium Liquid || culture Serial || dilutions || - || Correct || answer || ✔1. || liquid || culture
- || serial || dilutions
- || plate || on || pg || medium
- || UV || exposure GE || 11 || quiz- || Which || medium || (EMB || or || R2A) || corresponds || to || each || condition || listed || below || for || membrane || filter || methods || to || count || bacteria? incubated || at || room || temp incubated || at || 37* promotes || growth || of || slow-growing || heterotrophs inhibits || gram-positive || species || - || Correct || answer || ✔incubated || at || room || temp: || R2A incubated || at || 37*: || EMB promotes || growth || of || slow-growing || heterotrophs: || R2A inhibits || gram-positive || species: || EMB GE || 11 || quiz- || Name || 3 || characteristics || of || coliform || bacteria || - || Correct || answer || ✔ferments || lactose || with || acid || and || gas || formation rod-shaped facultatively || anaerobic GE || 11 || quiz- || once || a || sample || has || been || filtered, || filter || is || placed || in || which || orientation || on || the || agar? || - || Correct || answer || ✔bacteria || up P3 || quiz- || What || should || the || P3 || complementation || assay || streak || look || like || - || Correct || answer || ✔Two || parallel || upside || down || L's P3 || quiz- || Consider || this || branch || of || the || prodigiosin || biosynthetic || pathway:
answer || ✔malate || and || citrate || are || non-fermentable || carbon || sources, || thus || preventing || growth || of || fermentable || bacteria GE || 12 || quiz- || The || procedure || that || we || will || employ || for || isolating || bacteria || from || skin || will || sample.. || (transient || and/or || resident || species, || or || neither) || - || Correct || answer || ✔both || transient || and || resident || species GE || 12 || quiz- || Assign || a || medium || (RCMF || or || HBA) || to || each || of || these || two || bacterial || groups || that || will || be || isolated || from || the || skin Micrococcaceae Propionibacterium || - || Correct || answer || ✔micrococcaceae: || HBA Propionibacterium: || RCMF GE || 12 || quiz- || Assign || a || growth || condition || (aerobic || or || anaerobic) || to || each || of || these || two || bacterial || groups || that || will || be || isolated || from || the || skin. Micrococcaceae Propionibacterium || - || Correct || answer || ✔Micrococcaceae: || aerobic Propionibacterium: || anaerobic GE || 12 || quiz- || What || test || and || what || results || will || confirm || the || presence || of || members || from || two || these || groups? (a) || Micrococcaceae (b) || Propionibacterium || - || Correct || answer || ✔a. || gram || stain || that || indicates || aerobic || flora || with || gram || positive || cocci || in || clusters. || colonies || can || be || white, || grey, || golden, || or || yellow || and || be || hemolytic || or || non-hemolytic
b. || gram || stain || that || indicates || anaerobic || flora || with || gram || positive || rods || clumped || together. || colonies || can || be || brown/yellow, || pink || and || mucoid, || or || pink || with || scalloped || edge GE || 13 || quiz- || list || 3 || of || the || 5 || attributes || of || coliform || bacteria || - || Correct || answer || ✔facultatively || anaerobic rod-shaped gram || negative GE || 13 || quiz- || how || many || replicate || tubes || per || dilution || will || be || used || in || MPN || tests ||
- || Correct || answer || ✔ 5 GE || 13 || quiz- || what || is || considered || a || positive || reaction || in || a || coliform || MPN || tube || (2 || answers) || - || Correct || answer || ✔gas turbidity GE || 13 || quiz- || choose || the || medium || that || is || used || for || the || "presumptive" || coliform || test || - || Correct || answer || ✔LTB GE || 13 || quiz- || what || is || the || selective || component || of || the || fecal || coliform || MPN || test || - || Correct || answer || ✔high || temperature || (45.5* || C) MPN || (filter || method) || for || assessing || coliform || counts || is || limited || by || - || Correct || answer || ✔plate || counts || under || 0.2mL, || maximum || volume || of || 5mL || per || sample
When || membrane || filtering, || do || not || touch || - || Correct || answer || ✔any || surfaces || that || will || contact || the || water || sample || or || the || filter Membrane || filter || method || procedure || - || Correct || answer || ✔1. || unwrap || and || assemble || sterilized || filter || housing ||
- || sterilize || forceps || by || dipping || in || alcohol || quick || pass, || place || membrane || filter || on || glass || base, || place || reservoir || on || base, || clamp || two || parts, || attach || filter || flask || to || vaccum || source ||
- || add || measured || water || to || the || filter || resevoir || using || sterile || graduated || cylinder || or || pipette (small || filters || <5mL || need || to || be || condensed || on || the || filter, || first || add || your || sample || to || a || t || ube || of || 9mL || sterile || saline || then || filter || entire || quantity
- || apply || vaccum, || release || vaccum, || remove || filter || using || sterilized || forceps, || lay || filter || BACTERIA || SIDE || UP
- || Incubtate || EMB || plates || 37 || degrees || 24 || hours, || count || colored || dark || purple || to || black Incubate || R2A || plates || at || room || temp || for || 5 || days For || total || heterotrophic || bacteria || count || all || bacterial || colonies transient || species || - || Correct || answer || ✔generally || harmless || bacteria || that || provide || some || protective || capacity, || appear || as || a || result || of || contact || with || surroundings, || highly || variable || and || can || be || washed || off || easily resident || species || - || Correct || answer || ✔live || in || or || on || the || skin || utilizing || skin || sevretions || and || wastes || as || nutrient || sources
Bacterial || populations || of || skin || procedure || - || Correct || answer || ✔1. || place || sterile || sampling || cyclinder || on || area || to || be || sampled, || apply || pressure || to || ensure || no || leakage || of || wash || fluid, || pipette || 1mL || of || wash || fluid || into || cylinder, || rub || for || 1 || min || with || sterile || rod || glass
- || withdraw || into || pipette || and || dispense || into || empty || sterile || test || tube
- || perform || second || wash || of || same || area || and || pool || the || two || wash || samples, || estimate || total || volume || with || 5ml || pipette
- || make || 10 || fold || serial || diluations || of || combined || sample, || spread || 0.1 || mL || of || dilutions || onto || plates || of || HBA/RCMF || media
- || incubate || HBA || for || 2 || days, || place || RCMF || plates || in || anaerobic || jar || for || 7 || days
- || examine || colony || types HBA || plates || are || - || Correct || answer || ✔non || selective || heated || blood || agar || incubated || aerobically || and || allows || growth || of || micrococcaceae RCMF || plates || are || - || Correct || answer || ✔incubated || anaerobically || and || selects || for || species || of || propionibacterium Aerobic || flora || are || - || Correct || answer || ✔gram || positive || cocci, || often || in || clusters || but || not || chains, || colonies || are || white, || gray, || golden, || yellow, || hemolytic || or || non || hemolytic Anaerobic || flora || are || - || Correct || answer || ✔gram || positive || rod || or || club || shaped || cells, || often || clumped || in || groups || resembling || chinese || letters/picket || fences, || brown || yellow/pink
Cleaner || water || should || require || - || Correct || answer || ✔larger || volumes || and || few || dilutions, || contaminated || water || will || need || several || higher || order || dilutions For || volumes || greater || than || 1... || - || Correct || answer || ✔membrane || filtration || must || be || used, || after || filtration, || rinse || membrane || with || water, || ethanol || sterilize || gloves || before || folding || bacteria || side || of || membrane || localized || internally, || add || to || LTB || tubes || with || forceps For || volumes || of || 1 || mL || - || Correct || answer || ✔pipette || directly || from || water || source || into || each || of || the || 5 || LTB || tubes Fir || volumtes || less || than || 1 || mL || - || Correct || answer || ✔serial || dilutions || adding || 1 || mL || of || each || dilution || to || each || of || the || 5 || LTB || tubes After || examining || LTB || tubes || for || turbidity || and || gas || - || Correct || answer || ✔transfer || loop || of || broth || to || tube || of || BGLB, || do || not || shake, || make || sure || each || BGLB || tube || is || labeled After || examining || BGLB || tubes... || - || Correct || answer || ✔records || number || of || positives || for || each || dilution, || transfer || to || EC, || record || number || of || positives || for || each || dilution Rule || 1 || for || MPN || Index || - || Correct || answer || ✔To || select || the || three || dilutions || to || be || counted || for || the || MPN || index, || choose || the || highest || dilution || in || which || all || 5 || replicates || are || positive || and || the || next || two || succeeding || higher || dilutions
Rule || 2 || for || MPN || Index || - || Correct || answer || ✔If || no || dilution || has || five || positive || tubes, || the || dilution || with || the || highest || number || of || positives || and || the || next || two || higher || dilutions || are || used. Rule || 3 || of || MPN || - || Correct || answer || ✔After || choosing || the || three || dilutions || to || be || considered, || all || remaining || positives || in || higher || dilutions || are || counted || and || added || to || the || third || number || of || positives Rule || 4 || for || MPN || - || Correct || answer || ✔The || MPN || value || for || combination || not || appearing || on || the || table || may || be || estimated || by || MPN/100mL || = || ((total || # || of || positive || tubes)(100))/(square || root((combined || vol || of || sample || in || negative || tubes)(combines || volumte || of || sample || in || all || tubes)) B-hemolysis || - || Correct || answer || ✔indicates || a || clear || halo, || due || to || complete || lysis || of || red || blood || cells || in || the || colony alpha-hemolysis || - || Correct || answer || ✔indicates || a || greenish || halo, || caused || by || incomplete || lysis gamma-hemolysis || - || Correct || answer || ✔indicates || a || lack || of || hemolysis What || does || opportunistic || mean || - || Correct || answer || ✔doesn't || mean || the || host || is || diseased, || but || the || organism || could || cause || illness || in || a || susceptible || host || if || it || invades
PYR || test || is || composed || of || - || Correct || answer || ✔Group || A || streptococci, || enterococci, || and || some || micrococcaceae || posess || this || enzyme || and || can || be || detected || with || a || simple || disk || test pink || to || red || color || is || positive, || no || change || or || orange || is || negative Antibiotic || sensitivity || is || composed || of || - || Correct || answer || ✔sensitivity/resistance || are || particularly || useful || for || gram || pos || cocci, || a || paper || disk || impregnated || within || the || antibiotic || is || placed || on || a || solid || medium || on || which || heavy || inoculum || of || test || bacteria || has || been || spread, || the || bacteria || will || grow || to || form || a || lawn if || it || is || sensitive, || a || zone || of || clearing || will || form || around || the || disk Antibiotic || test || steps || - || Correct || answer || ✔scape || 2-4 || medium || colonies || from || a || plate || and || suspend || the || cells || in || about || 0.5mL || of || sterile || water || in || a || sterile || tube, || dip || a || sterile || cotton || swab || into || suspension, || and || twist || the || wet || cotton || swab || on || the || inside || of || the || tube || to || remove || some || of || the || liquid. || Spread || the || culture || evenly || across || the || blood || agar || plate || by || streaking || adn || rolling || the || swab || back || and || foth, || turn || plate || 90 || degrees, || spread/roll || across || whole || surface obtain || dispenser || from || fridge Use || sterilized || forceps || and || pull || disk || and || put || it || on || the || plate inoculate || for || 24-48 || hours || at || 37 || degrees, || measure || the || diameter || of || teh || cleared || zone growth || of || 5% || NaCl || is || composed || of` || - || Correct || answer || ✔tests || for || tolerance || to || salt, || used || to || distinguish || staphylococci || from || other || catalase || pos || gram || cocci Equivalent || growths || shows || positive, || less || growth || in || NaCl || is || negative
Orthinine || decarboxylase || is || composed || of... || - || Correct || answer || ✔tests || for || presence || of || decarboxylase || enzymes || that || are || required || for || breakdown || of || a.a. || to || amines || and || carbon || dioxide purple || is || positive, || yellow || or || bronze || is || negative Bile || esculin || is || composed || of... || - || Correct || answer || ✔Esculin || is || a || glucoside || composed || of || glucose || adn || 10 || membered || aromatic || ring || (esculetin) enterococci || and || group || D || streptococci || produce || an || enzyme || that || cleaves || the || esculetin || from || glucose The || enzymes || can || tolerate || bile || salts, || are || isolated || from || intestine, || and || medium || is || selective Esculetin || liberated || from || esculin || reacts || with || ferric || ions || in || the || medium || to || produce || dark || brown || coloration, || must || be || fully || darkened || to || show || positive || result Voges-Proskauer || test || is || composed || of... || - || Correct || answer || ✔a || number || of || acidic || products || are || generated || from || the || fermentation || of || carbohydrates. || Some || convert || their || acids || to || dialcohol || 2,3 || butanediol || (fermentation). || An || intermediate || in || butanediol || production || is || the || compound || acetoin, || detected || with || broth || in || which || cells || ferment || glucose. a || positive || result || will || turn || red/pink, || if || no || red || is || detected, || must || wait || an || hour, || negative || results || are || yellow || or || copper Acid || from || lactose/mannitol || - || Correct || answer || ✔prokaryotes || can || ferment || one || or || more || carbs, || producst || or || intermediates || include || acids || and || gases, || heavily || inoculate || a || tube || of || BP || plus || carb || broth || in || your || culture || and || incubate || no || shaking, || check || for || acid || production || via || purple || PH || to || yellow || change
compounding || metabolic || demands requires || large || batches || to || grow nitrosomonas || in || pure || culture || are || indicated || by || - || Correct || answer || ✔reduction || of || ammonia || or || teh || accumulation || of || nitrate nitrobacter || in || pure || culture || are || indicated || by || - || Correct || answer || ✔depletion || of || nitrite || or || accumulation || of || nitrate if || both || nitrite || oxidizers || and || ammonia || oxidizers || are || present || - || Correct || answer || ✔no || nitrite || builds || up to || test || for || presence || of || nitrite/ || ammonia || oxidizers || - || Correct || answer || ✔block || the || activity || of || nitrite || oxidizers || so || that || ammonia || oxidation || can || be || detected || by || increase || in || nitriate accomplished || by || adding || chlorate || which || inhibits || oxidation || of || nitrite || without || affecting || ammonia || oxidizers nitriate || concentration || is || high || in || the || - || Correct || answer || ✔flakss || containing || nitrite || medium, || so || they || must || be || diluted || 100x ammonium || media || - || Correct || answer || ✔no || dilution || needed || on || the || initial || sample || as || there || is || no || added || nitrate
the || number || of || spec || tubes || - || Correct || answer || ✔depends || on || how || many || samples || you || diluted || and || how || many || samples || you || will || use || undiluted || for || the || nitrite || analysis an || inactivating || mutation || in || any || one || gene || of || the || pathway || - || Correct || answer || ✔will || prevent || the || synthesis || of || teh || final || product auxotroph || - || Correct || answer || ✔mutant || unable || to || make || a || factor || required || for || growth rescued || means || - || Correct || answer || ✔the || WT || phenotype || can || be || restored || if || the || mutant || is || supplied || with || an || intermediate || compound || that || is || positioned || downstream || of || the || pathway's || block can || do || this || by || supplementing || growth || on || solid || medium A || disruption || in || a || pathway || often || leads || to || a || buildup || of || precursor || preceding || the || block || - || Correct || answer || ✔precursor || will || leach || out || of || teh || cell || and || into || the || surrounding || medium A || neighboring || mutant || may || beable || to || absorb || the || leached || intermediate, || and || if || the || defective || step || is || upstream || of || the || donor... || - || Correct || answer || ✔the || recipient || may || be || able || to || convert || the || intermediat || ultimately || to || the || final || product || of || the || biosynthetic || pathway || = || crossfeeding, || a || type || of || complementation || analysis UV || mutagenesis || energy || label || - || Correct || answer || ✔X100uJ/CM^
Cyanobacteria || may || be || divided || into || three || groups || - || Correct || answer || ✔1. || unicellular || forms || that || no || specialized || cells || and || little/no || motility
- || unbranched || filamentous || forms || with || no || specialized || cells, || move || by || gliding || except || when || surrounded || by || sheath
- || filamentous || forms || which || produce || occasional || or || numerous || heterocysts, || resting || spores, || and || gliding || motility || seen || only || periodically || in || special || short || filaments Sources || for || denitryging || bacteria || - || Correct || answer || ✔garden || soil, || lagoon || water, || anaerobic || mud sources || for || cyanobacteria || - || Correct || answer || ✔windogradsky || or || hot || spring || columns, || soil || samples, || pond, || hot || springs medium || for || cyanobacteria || - || Correct || answer || ✔BG-11, || SWBG-11, || 7.5 || pH, || mineral || medium || that || will || not || support || heterotrophs || due || to || absence || of || organic || carbon enrichment || for || cyanobacteria || - || Correct || answer || ✔amphoteracin || B || added || to || initially || inhibit || grwoth || of || msot || eukaryotic || algae, || thus || selecting || for || cyanobacteria || in || the || inoculum isolation || for || cyanobacteria || - || Correct || answer || ✔standard || manual || dilution || methods for || gliding || ones, || manual || isolation || is || needed in || ziplock || bags || inverted
for || purple || non-sulfur || bacteria || - || Correct || answer || ✔carbon || sources || are || organic, || sulfate || is || low, || DCMU || selects || against || cyanobacteria || by || inhibiting || photosystem || II, || high || organic || concentration || also || inhibits || cyanobacteria medium || for || purple || non || sulfur || - || Correct || answer || ✔DCMU || and || vitamins, || either || malate || or || citrate || as || non || fermentable || carbon || sources || (prevents || competition || from || heterotrophic || fermentative || bacteria) isolation || for || non || sulfur || - || Correct || answer || ✔agar || solidified || medium, || incubate || in || Gaspaks You || measure || no || bacterial || cells || from || filtering || 5 || ml || of || water, || but || you || need || to || report || it || as || number || of || bacteria || per || 100ml? || - || Correct || answer || ✔< || 20 || cells/100ml Cyanobacteria || use || light || for || energy. || Describe || them... || - || Correct || answer || ✔- || heterogenous || group || of || aerobic, || green || photoautotrophic || bacteria, || use || light || to || drive || incorporation || of || CO
- || phototrophic || metabolism || nearly || identical || to || that || of || algae, || plants
- || share || chlorophyll || a
- || 5 || groups || distinguished || by || morphology || and || mode || of || division filamentous || - || Correct || answer || ✔heterocysts, || vegetative || cell || photosynthesizes || sugars, || heterocyst || fixes || nitrogen
