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Blood collection techniques, Study notes of Human Biology

Preparation for veinous blood -steps taken -anticoagulant used Collection of capillary blood

Typology: Study notes

2021/2022

Uploaded on 09/22/2023

Riya3003
Riya3003 🇮🇳

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Blood collection techniques
Blood can be collected from antecubital vein on the forearm and finger
It can be collected through syringe and needle / vacuum tube
Venous blood is preferred
Preparation for veinous blood
Steps
Vein should be easily accessible1.
Site should be cleaned with 70% IPA and not touched afterwards2.
tourniquet should be used 3-4 inches above site. Patient should make a fist3.
Hang down the arm and gently slapping the arm also works4.
clean hands and information on tube1.
keep the cap on until used and check the syringe2.
Keep the needle up and puncture the skin n a parallel way3.
appearance of blood on the hub of the needle indicates that needle has successfully
entered the vein
4.
tourniquet should be removed5.
withdraw the piston slowly and apply pressure over the puncture site6.
release the blood inside the receiver and cap it7.
Destroy the needle8.
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Blood collection techniques

Blood can be collected from antecubital vein on the forearm and finger It can be collected through syringe and needle / vacuum tube Venous blood is preferred

Preparation for veinous blood

Steps

  1. Vein should be easily accessible

  2. Site should be cleaned with 70% IPA and not touched afterwards

  3. tourniquet should be used 3-4 inches above site. Patient should make a fist

  4. Hang down the arm and gently slapping the arm also works

  5. clean hands and information on tube

  6. keep the cap on until used and check the syringe

  7. Keep the needle up and puncture the skin n a parallel way appearance of blood on the hub of the needle indicates that needle has successfully entered the vein

  8. tourniquet should be removed

  9. withdraw the piston slowly and apply pressure over the puncture site

  10. release the blood inside the receiver and cap it

  11. Destroy the needle

Collection of capillary blood

In adults capillary blood sample can be obtained from the lateral side of tip of the 3rd or 4th finger

For Infants,the sample can be obtained by a deep puncture of the plantar surface of the heel.

samples can be used for making peripheral blood films ,performing hematocrit/Hb and point of care testing

Colour of the cap Anticoagulant Uses

Purple EDTA Blood count

Red - Test which need serum

Blue Sodium Citrate Coagulation test

Green Fluoride Blood sugar