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Bloodstain Indication: Kastle-Meyer Test, Study Guides, Projects, Research of Genetic Engineering

Hood CollegeGenetic Engineering

This test is utilized as a preliminary screening test for blood. SAFETY CONSIDERATIONS. Hydrogen Peroxide 30% - Danger! Corrosive! Phenolphthalein - Caution!

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DNA Analyst Training
Laboratory Training Manual
Protocol 2.15
Bloodstain Indication: Kastle-Meyer Test
This laboratory protocol (or part thereof) has been provided as an example of a laboratory SOP,
courtesy of the Illinois State Police. It has been included for training and example purposes only.
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DNA Analyst Training

Laboratory Training Manual

Protocol 2. Bloodstain Indication: Kastle-Meyer Test

This laboratory protocol (or part thereof) has been provided as an example of a laboratory SOP, courtesy of the Illinois State Police. It has been included for training and example purposes only.

INTRODUCTION

This test is utilized as a preliminary screening test for blood.

SAFETY CONSIDERATIONS

Hydrogen Peroxide 30% - Danger! Corrosive!

Phenolphthalein - Caution! Irritant!

Potassium Hydroxide - Danger! Corrosive!

Zinc powder or dust in contact with water or damp air evolves hydrogen. The heat of reaction is sufficient that the hydrogen may ignite. Therefore, zinc should not be discarded in the wastebasket. The following procedure should be followed for less than 20 grams of zinc dust:

  1. Follow standard laboratory chemical handling practices and work in the hood with the hood on, wearing safety glasses and rubber gloves. With the zinc in a large beaker, add small amounts of concentrated hydrochloric acid with a pipet. The solution will bubble and give off heat. Proceed slowly. Allow time for the bubbling and heat to dissipate before adding more acid. Continue slowly adding acid until no more bubbles are formed and no gray powder is visible (about 3 mls. HCl for 1 gram of zinc).
  2. When all the zinc has dissolved (forming soluble zinc chloride), cautiously neutralize the acid solution by adding small amounts of sodium carbonate. Again, foaming will occur. Continue slowly adding sodium carbonate until no more bubbling occurs (about 2 g. sodium carbonate for 1 gram of zinc). At this point, all the zinc should now be in the form of zinc carbonate, a white precipitate.
  3. The zinc carbonate may be filtered out of solution and disposed of in a trash can since zinc carbonate is nontoxic.

PREPARATIONS

Stock Solution: Phenolphthalein 2 g. Potassium hydroxide 20 g. Distilled water 100 ml. Zinc dust 20 g.

Mix, add a few boiling chips and boil under reflux 2-3 hours or until the solution has lost all its pink color. Cool and decant into a bottle containing some zinc to keep it in the reduced form.

pdi_lab_pro_2.15.pdf President's DNA Initiative - DNA Analyst Training Page 1 of 2