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Chapter 9 Review - Biology 1090 SLCC Questions with
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Gene Expression - ANSWERSGenes carry instructions for synthesizing proteins. Gene expression involves turning a gene on that includes the process of transcription and translation Describe how messenger RNA (mRNA) is synthesized during the process of transcription - ANSWERSTranscription occurs in the nucleus of eukaryotic cells when and RNA polymerase enzyme binds to the promoter, located at the start site of a gene, and makes an mRNA that is complementary to the DNA gene. Describe how proteins are synthesized during the process of translation - ANSWERSTranslation occurs in the cytoplasm of eukaryotic cells and involves mRNA, ribosomes, and transfer RNA (tRNA). Messenger RNA carries the code from the DNA, and ribosomes are the site where amino acids are assembled to synthesize proteins. Transfer RNA carries amino acids, which binds to triplet new nucleotide sequence is on the M RNA called codons. A particular tRNA carries a specific amino acid. Each tRNA has its unique anticodon that binds to the codon and carries instructions for its particular amino acid. Use the genetic code to identify the amino acid for which a DNA segment or a codon codes - ANSWERSThe amino acid coded for by a particular codon can be determined using the genetic code Define the term mutation, and explain how mutations can affect protein structure and function - ANSWERSMutations are changes to DNA sequences that can affect protein structure and function Neutral mutations are changes to the DNA that do not result in a different amino acid being incorporated. Insertions or deletions of nucleotides can result in frameshift mutation said change the protein drastically. Explain why and how some genes are expressed in some cell types but not others - ANSWERSA given cell type express is only a small percentage of the gene that an organism possesses. Turning the expression of a gene up or down is accomplished in different ways in prokaryotes and eukaryotes. Prokaryotes typically block the promotor with a repressor
protein to keep gene expression turned off. Eukaryotes regulate gene expression in any of five ways: 1 increasing transcription through the use of proteins that stimulate RNA polymerase binding. 2 varying the time that DNA spends in the uncondensed, active form. 3 altering the mRNA lifespan. 4 slowing down or speeding up translation. And 5 affecting the protein lifespan. Outline the process of cloning a gene using bacteria - ANSWERSBacteria can be used to clone genes by placing the gene of interest into a plasmid, which then makes millions of copies of the gene as the plasmid replicates itself inside it's bacterial host. You can then express the gene by transcribing an mRNA copy and translating the mRNA into a protein. Describe how foods are genetically modified, and list some of the health and environmental concerns surrounding this practice - ANSWERSA Ti plasmid or gene gun can be used to insert a particular gene into plant cells. Although there have been no documented incidents of negative health effects from GM food consumption there is concern that GM foods may be unhealthy. Concerns about GM crops include their impacts on surrounding organisms, the evolution of resistance is, and transfer of modify genes to wild and weedy relatives. Describe the science behind and significance of stem cells, the Human Genome Project, gene therapy, and cloning. - ANSWERSStem cells are undifferentiated cells that can be reprogrammed to act as a variety of cell types. Stem cells may someday allow scientist to treat degenerative diseases. The Human Genome Project resulted in the production of the complete DNA sequence of humans and several other organisms. Gene therapy involves replacing defective genes on their products in an embryo or in the affected adult tissue. Gene therapy is considered experimental but may hold promise one scientist determine how to target jeans to the right locations and express them in the proper amounts. Nuclear transfer has been used to clone animals with desirable agricultural traits. It may someday be possible to clone humans, but it is unclear if he's humans would be healthy.