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A review sheet for exam iv of bio 120, covering chapters 18, 19, 20, 43, and 47. It includes key concepts related to bacterial and eukaryotic gene expression regulation, dna technology applications, and immunology.
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Chapter 18 Describe the trp and lac operons and how they function. Include any repressors, corepressor, and inducers. What is a bacterial chromosome made of? What shape is it? What is an inducer? Repressor? Corepressor? What is transformation? (Like we did in lab) What is CAP? How does it work to regulate the lac operon? What is heterochromatin? How is DNA modified to regulate expression? How is chromatin modified to regulate gene expression? What is a control element and how do they regulate gene expression? What are enhnacers? What do transcription factors do? What is alternative splicing? List three mechanisms of post-transcriptional regulation and how each works. At what level do eukaryotes regulate gene expression? Transcription or translation?
Chapter 19 Describe the lytic and lysogenic cycles in phages. What are prions? Viroids? What is a plasmid? What are bacteriophages? What are viruses made of? What is reverse transcriptase used for? How are animal viruses grouped/classified? What is a capsid? What is an envelope? What is a provirus?
Chapter 20 Name several applications of DNA technology. What are sticky ends? What are nucleic acid probes used for? Describe PCR and DNA sequencing. What is a vector? How does gel electrophoresis work? What are RFLPs? What is the Ti plasmid? Describe DNA fingerprinting. Describe Southern blotting. What does transgenic mean? What function do restriction enzymes and DNA ligase have? What is a pharm animal? What is involved in gene therapy?
Chapter 43 Describe the functions of B and T cells. What do B and T stand for? Where do each mature? How are they activated? Name the 5 different antibody classes and what they do. What are interferons? Define antigen and antibody. What are NK cells? Describe active and passive immunity and examples of each. Name the 3 lines of defense against infection and describe what occurs in each. What is humoral vs. cellular-mediated immunity? What is the difference between class I and II MHC molecules? What is phagocytosis? What is the purpose of a vaccination? How do macrophages function? When is histamine released? Describe differences between the primary and secondary immune response. What cell does HIV infect? What is complement?
Chapter 47 What are the following structures/stages of development? Blastula Gastrula Organogenesis Gastrulation Cleavage Zygote Fertilization Name the 3 germ layers and what develops from each. What does the acrosome do? What causes the slow and fast blocks to polyspermy? What is the vegetal pole? Animal pole? What are cell differentiation and morphogenesis? Name the extraembryonic membranes and how they function. What are somites?