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A series of multiple-choice and short-answer questions related to biochemistry, specifically focusing on glycolysis, the citric acid cycle, and beta-oxidation. It includes questions about atp and nadh production, enzyme functions, and the steps involved in these metabolic pathways. The document also provides verified answers, making it a useful resource for students studying biochemistry. It covers key concepts such as substrate-level phosphorylation, anaerobic conditions, and the role of various enzymes and coenzymes in cellular respiration. The questions are designed to test understanding of the biochemical processes and their regulation, offering a comprehensive review of core metabolic pathways.
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True or False: The ΔG value for a particular reaction is - 78kJ/mol. This value indicates the reaction is NOT spontaneous. FALSE True or false: A biochemical metabolic pathway is a single reaction that converts starting material into products. FALSE
3 / 3 pts True or false: Three ATP molecules are used in the energy-investing stage of glycolysis. FALSE True or False: The citric acid cycle occurs in the absence of oxygen. FALSE True or false: The β-oxidation pathway degrades both even-numbered and oddnumbered fatty acids. TRUE
3 / 3 pts In converting glucose to pyruvate, how many ATP or NADH are produced (or required) in the 1,3- bisphosphoglycerate to 3-phosphoglycerate step in glycolysis?
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2 ATP produced 1 ATP required 1 NADH produced 2 NADH and 1 ATP produced None of the above 2 ATP PRODUCED
3 / 3 pts In converting glucose to pyruvate, how many ATP or NADH are produced (or required) in the phosphoenolpyruvate to pyruvate step in glycolysis? 1 ATP required 1 ATP produced 2 ATP produced 2 ATP required None of the above 2 ATP PRODUCED
3 / 3 pts What is the primary reactant of glycolysis? Glucose Glucose- 6 - phosphate Fructose Fructose- 6 - phosphate Hexokinase GLUCOSE In glycolysis, when glucose enters a cell, it is immediately phosphorylated to form glucose- 6 - phosphate. The phosphate donor in this reaction is ATP, and the enzyme is.
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None of the above LACTATE How many steps are in the glycolysis pathway? 3 6 10 12 14 10 The reactions of glycolysis take place in/on the. Mitochondria Vacuoles Ribosome Cytosol None of the above CYTSOL How is ATP used in the initial steps of glycolysis? Isomerization reaction Phosphorylation reaction Protein production
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3 / 3 pts Lipid phosphorylation None of the above PHOSPHORYLATION REACTION Which of the following is a cyclic pathway? A. CAC B. PDH C. Glycolysis D. Oxidative phosphorylation E. β-oxidation A.CAC How many ATP molecules does NADH yield after oxidative phosphorylation?
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3 / 3 pts E. Succinyl-CoAC.SUCCINATE Pyruvate conversion to acetyl-CoA production occurs in the. Lysosomes Vacuoles Nucleus Mitochondria None of the above MITOCHONDRIA Cellular respiration is the process of cells taking in and producing to generate energy. A. Glucose; NADH B. FAD; FADH 2 C. CO 2 ; ATP D. NAD+; NADH E. O 2 ; CO 2 E.O2; CO How many phases are in the complete degradation of glucose via cellular respiration?
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3 / 3 pts The PDH complex has three enzymes, which are referred to as: P, D, and H E1, E2, and E Enzyme A, Enzyme B, and Enzyme C Enzyme a, Enzyme b, and Enzyme c None of the above E1, E2, AND E Of the following, which coenzyme(s) is (are) necessary for β-oxidation? A. O 2 B. FAD C. GTP D. Both A and B E. None of the above B.FAD
5 / 5 pts D. α-spiral E. β-spiral E. β-spiral (Short answer) In one step of the glycolysis pathway, a six-carbon compound is broken into two three-carbon fragments, only one of which can be further degraded in the glycolysis pathway. What happens to the other three-carbon fragment? In step 5 of glycolysis, glucose (six-carbon) is converted to GAP and DHAP (both 3 - carbons). GAP goes on in the glycolysis pathway. The other, DHAP, cannot go on through glycolysis directly; it is converted to GAP by triose phosphate isomerase. Question 27 5 / 5 p (Fill in the blank) Use the following word bank to fill in the appropriate blanks. Not all words will be used; words can be used more than once. Word bank: one, two, three, four, five, six Glucose contains (A) carbon atoms. The oxidation of a glucose molecule utilizes (B) ATP. Later, the (C) three-carbon products of glycolysis produce (D) ATP by direct phosphorylation, which gives a net yield of two ATP. In addition, (E) NADH are produced in glycolysis. A) six B) two
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5 / 5 pts (Short response) Consider the fatty acid which has 20 carbons (below). A. (2 pts) How many cycles of β-oxidation are required for catabolism? Briefly explain. B. (2 pts) How many acetyl-CoA are produced during catabolism? Briefly explain. C. (1 pts) How many NADH and FADH 2 are produced from β-oxidation (only) of this molecule? A. 9 cycles are required for oleic acid. Each cycle removes two carbon units. ON the final cycle, two acetyl groups are produced. B. There are 10 acetyl-CoA groups produced. Two acetyl-CoA groups on the last cycle. C. There are 9 NADH and 9 FADH2 molecules produced. One per cycle Short Essay: (10 points each)