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Biotechnology
Biotechnology
L a b o r a t o r y
L a b o r a t o r y
DNA Extraction from Fruit
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DNA Extraction from Fruit: A Biotechnology Lab Activity, Study notes of Biotechnology
In this biotechnology lab activity, students extract DNA from different fruits and compare the amount of DNA collected. The activity aims to help students understand the structure and function of DNA, its presence in various organisms including food, and the process of extracting DNA for scientific use.
What you will learn
- How is DNA extracted from fruits?
- What is the role of DNA in living organisms?
- What are the differences in DNA extraction from different fruits?
Typology: Study notes
2021/2022
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Biotechnology Outreach Program
B i o t e c h n o l o g y B i o t e c h n o l o g y
L a b o r a t o r y L a b o r a t o r y
DNA Extraction from Fruit
DNA Extraction
Introduction
Students extract a visible mass of DNA from 4 different fruits and visually compare the amount of the DNA collected. Attached readings and activities will illustrate the structure and function of DNA. This activity is designed to give students basic concepts that are vital for understanding agricultural biotechnology:
- Understand DNA is in the cells of all living organisms including in the foods we eat.
- Determine how each of the ingredients in the protocol help extract DNA.
- DNA can be extracted from cells for scientific use.
- Extract a visible mass of DNA from fruit tissue. In this extraction activity we have the students compare the papaya from 4 different fruits. Students find this more interesting than using a single fruit and it allows the instructor to introduce more concepts. Questions that you may want to explore include:
Hawaii Standards
- SC.BS.4.2 Describe different cell parts and their functions.
- SC.BS.4.5 Describe the components and functions of a variety of macromolecules active in biological systems.
Advance Preparation
- Prepare DNA Extraction Buffer: 100 ml of shampoo (do not use shampoos with conditioner or baby shampoo) 15 g of table salt Water Mix 100 ml of shampoo and table salt. Add water to make a final volume of 1000 ml.
- Obtain Precipitation Buffer (95% ethanol or isopropyl) and store in the freezer.
- Peel skin off ripe fruit and cut fruit pieces into 1 inch squares and place each square into snack-sized Ziplock bags.
Materials (For each person)
10 ml of DNA Extraction Buffer 5 ml of Precipitation Buffer 2.5 cm square piece of fruit in Ziplock bag 15 ml test tube Filter cup- 2 oz. plastic cup with a filter that is held in place with a piece of rubberband Plastic pipette Gloves Safety glasses Glass rod or chopstick 1.5 ml microtube Molecular grade water Waterproof marker
Control
Use one set of supplies to complete the procedure, but add no fruit.
fibers, somewhat like cotton candy that will spool onto
the glass rod.
DNA Extraction Discussion Questions