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Prof Janyl Jumadinova BIO300/CMPSC300 Mutation - Spring 2016 - Allegheny College
Typology: Exercises
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Name: ________________________ BIO300/CMPSC Mutation - Spring 2016 As you know from lecture, there are several types of mutation: DELETION (a base is lost) INSERTION (an extra base is inserted) Deletion and insertion may cause what’s called a FRAMESHIFT , meaning the reading “frame” changes, changing the amino acid sequence. POINT MUTATION (one base is substituted for another) If a point mutation changes the amino acid, it’s called a MISSENSE mutation. If a point mutation does not change the amino acid, it’s called a SILENT mutation. If a point mutation changes the amino acid to a “stop,” it’s called a NONSENSE mutation. Complete the boxes below. Classify each as either Deletion, Insertion, or Substitution AND as either frameshift, missense, silent or nonsense (hint: deletion or insertion will always be frameshift). Original DNA Sequence : T A C A C C T T G G C G A C G A C T mRNA Sequence: A U G U G G A A C C G C U G C U G A Amino Acid Sequence: MET - TRP- ASN - ARG- CYS - (STOP) Mutated DNA Sequence #1: T A C A T C T T G G C G A C G A C T What’s the mRNA sequence? A U G U A G A A C C G C U G C U G A What will be the amino acid sequence? MET - (STOP) Will there likely be effects? YES What kind of mutation is this? POINT MUTATION- NONSENSE Mutated DNA Sequence #2: T A C G A C C T T G G C G A C G A C T What’s the mRNA sequence? A U G C U G G A A C C G C U G C U G A What will be the amino acid sequence? MET - LEU - GLU– PRO-LEU-LEU Will there likely be effects? YES What kind of mutation is this? INSERTION - FRAME SHIFT Mutated DNA Sequence #3: T A C A C C T T A G C G A C G A C T What’s the mRNA sequence? A U G U G G A A U C G C U G C U G A What will be the amino acid sequence? MET-TRP-ASN- ARG- CYS (STOP) Will there likely be effects? NO What kind of mutation is this? POINT MUTATION– SILENT Mutated DNA Sequence #4: T A C A C C T T G G C G A C T A C T What’s the mRNA sequence? A U G U G G A A C C G C U G A U G A (Circle the change) What will be the amino acid sequence? MET-TRP- ASN- ARG- (STOP) Will there likely be effects? YES What kind of mutation is this? POINT MUTATION – NONSENSE
Original DNA Sequence : T A C A C C T T G G C G A C G A C T mRNA Sequence: Amino Acid Sequence: Mutated DNA Sequence #5: T A C A C C T T G G G A C G A C T What will be the corresponding mRNA sequence? A U G U G G A A C C C U G C U G A What will be the amino acid sequence? METHIONINE-TRYPTOPHAN-ASPARAGINE- PROLINE - ALANINE Will there likely be effects? YES What kind of mutation is this? DELETION – FRAME SHIFT