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EBIO 1210 Exam 4 Study Guide: Genetics and Molecular Biology, Exams of Biology

This study guide covers key concepts in genetics and molecular biology, including inheritance patterns, gene linkage, recombination frequencies, dna structure and replication, and the central dogma of molecular biology. It provides a comprehensive overview of these topics, with multiple-choice questions and answers to test understanding. The guide is particularly useful for students preparing for ebio 1210 exam 4.

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2024/2025

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EBIO 1210 Exam 4
Latest Updated Exam Study Guide
2025/2026.
Fully Certified by Expert
Combinations of alleles that are non-parental are called.... - ansRecombinant
Genes located on the same chromosome that tend to be inherited together are called.... -
ansLinked genes
What is the advantage of doing a test cross vs. an F1xF1 cross? - ansWe know the patterns of
heredity
We can isolate what is actually going on
What is the mechanism for recombining alleles? - ansCrossing over in Meiosis I
Genetic map of a chromosome based on recombination frequencies - ansLinkage Map
One map unit = ____% recombination frequency - ans1%
Independent assortment does NOT (always) apply to genes on the same chromosome. Why
not?
A. Allele combinations on a chromosome can never be broken up
B. A combination of alleles on one chromosome is less likely to be broken up than a
combination of alleles from multiple chromosomes
C. Non-homologous chromosomes pair up during meiosis
D. Meiosis makes it impossible to create non-parental
combinations of alleles in gametes. - ansB. A combination of alleles on one chromosome is
less likely to be broken up than a combination of alleles from multiple chromosomes
Why would some genes be inherited neither completely together nor completely
independently? - ansRecombination of linked genes
Suppose we are investigating two characters (e.g.,body color and wing type) in a study of
heredity. If the ratio in the offspring of parental types : recombinant types is intermediate
between the predictions of dependent assortment and independent assortment, then we should
conclude that:
A. meiosis has been disrupted in the parents used to produce the observed offspring
B. the parents used to produce the offspring have cancer
C. Mendel's law of segregation has been violated
D. This outcome should never happen because Mendel's law of independent assortment holds
for any two genes we consider
E. The genes responsible for the phenotypes we are observing are "linked," i.e., located near
each other on the same chromosome - ansE. The genes responsible for the phenotypes we are
observing are "linked," i.e., located near each other on the same chromosome
How can information about recombination frequencies be used to make a genetic map? -
ansAssume that genes that are farther apart will show higher recombination frequencies
A genetic map of chromosomes based on recombination frequencies - ansLinkage map
What is the formula for recombination frequency? - ans(# of recombinants/ total # of
Offspring) x 100
In experiments with two fruit fly characters like those we just learned about, suppose that the
raJo of the two parental types and the two recombinant types was 1:1:1:1. What would the
recombination frequency be?
A. 25%
B. 50%
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Combinations of alleles that are non-parental are called.... - ansRecombinant Genes located on the same chromosome that tend to be inherited together are called.... - ansLinked genes What is the advantage of doing a test cross vs. an F1xF1 cross? - ansWe know the patterns of heredity We can isolate what is actually going on What is the mechanism for recombining alleles? - ansCrossing over in Meiosis I Genetic map of a chromosome based on recombination frequencies - ansLinkage Map One map unit = ____% recombination frequency - ans1% Independent assortment does NOT (always) apply to genes on the same chromosome. Why not? A. Allele combinations on a chromosome can never be broken up B. A combination of alleles on one chromosome is less likely to be broken up than a combination of alleles from multiple chromosomes C. Non-homologous chromosomes pair up during meiosis D. Meiosis makes it impossible to create non-parental combinations of alleles in gametes. - ansB. A combination of alleles on one chromosome is less likely to be broken up than a combination of alleles from multiple chromosomes Why would some genes be inherited neither completely together nor completely independently? - ansRecombination of linked genes Suppose we are investigating two characters (e.g.,body color and wing type) in a study of heredity. If the ratio in the offspring of parental types : recombinant types is intermediate between the predictions of dependent assortment and independent assortment, then we should conclude that: A. meiosis has been disrupted in the parents used to produce the observed offspring B. the parents used to produce the offspring have cancer C. Mendel's law of segregation has been violated D. This outcome should never happen because Mendel's law of independent assortment holds for any two genes we consider E. The genes responsible for the phenotypes we are observing are "linked," i.e., located near each other on the same chromosome - ansE. The genes responsible for the phenotypes we are observing are "linked," i.e., located near each other on the same chromosome How can information about recombination frequencies be used to make a genetic map? - ansAssume that genes that are farther apart will show higher recombination frequencies A genetic map of chromosomes based on recombination frequencies - ansLinkage map What is the formula for recombination frequency? - ans(# of recombinants/ total # of Offspring) x 100 In experiments with two fruit fly characters like those we just learned about, suppose that the raJo of the two parental types and the two recombinant types was 1:1:1:1. What would the recombination frequency be? A. 25% B. 50%

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C. 75%

D. 100%

E. not enough information given - ansB. 50% What can explain a recombination frequency of 50%? - ansGenes that are far apart on the same chromosome behave as if they are unlinked Can multiple crossover events PER chromosome occur? - ansYes. And these an affect observed recombination frequencies. What is a nucleic acid? - ansA polymer made of nucleotide monomers. What is the 5' and 3' end of DNA? - ans5' End- Sugar phosphate backbone 3' End- Sugar (deoxyribose) A nucleotide consists of: - ans1. A nitrogenous base

  1. A sugar
  2. A phosphate group What are the three fundamental building blocks of DNA? - ans1. Nitrogenous base
  3. 5-carbon sugar
  4. Phosphate group The sugar phosphate backbone is around the outside of a double stranded molecule with nitrogenous bases in the middle Name the 4 different nitrogenous bases: - ans1. Thymine
  5. Adenine
  6. Cytosine
  7. Guanine Different sequences of the 4 possible nucleotides make up the different genetic code of every living organism What is Chargaff's Rule? - ans% Adenine = % Thymine % Cytosine = % Guanine Suppose that 22% of an organism's genome is the nucleotide guanine (G). What else do you know about the organism? A. Nothing B. It must have 22% A C. It must have 22% C, 22% T, 22%A, and 12% something else D. It must have 22% C, 28% A, and 28% T E. It must have 22% A, 28% C, and 28% T - ansD. 22% C, 28% A, and 28% T The phosphate group in a single nucleotide is attached to the ____' carbon atom of the sugar.
  • ans5' How do two strands of DNA form a double helix? - ans****The strands of DNA run in an antiparallel manner
  • The phosphate group in a single nucleotide is attached to the 5' carbon atom of the sugar
  • Adjacent nucleotides are linked phosphate to 3' carbon atom--- This forms the backbone
  • Antiparallel arrangement allows proper base pairing During what phase does DNA replication occur? - ansS Phase of Interphase

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A. Open double helix---> Build RNA primer ---> Add DNA to 3' end of RNA primer ---> Replace RNA primers with DNA B. Open double helix---> Build DNA primer---> Add DNA to 5' end of DNA primer C. Open double helix---> Build RNA primer---> Add RNA to 3' end of RNA primer - ansA.

  1. Open double helix
  2. Build RNA primer
  3. Add DNA to 3' end of RNA primer
  4. Replace RNA primers with DNA In DNA replication, the difference between the "leading" strand and the "lagging strand is that... A. The leading strand is made of DNA whereas the lagging strand is made of RNA B. A primer is used in the synthesis of only the lagging strand (and not the leading strand) C. Synthesis of the lagging strand begins after synthesis of the leading strand is complete D. The leading strand is the template strand and the lagging strand is the newly synthesized strand E. The leading strand is synthesized continuously; the lagging strand is synthesized as a series of fragments - ansE. The leading strand is synthesized continuously; the lagging strand is synthesized as a series of fragments *** Primer is used in both **** They are synthesized simultaneously What explains how a mutant allele could make a deer albino? A. The mutation prevents DNA replication in the deer B. The mutation prevents meiosis in the deer C. The mutation creates a faulty version of a pigment-synthesizing enzyme D. The mutation prevents mitosis in the deer - ansC. The mutation creates a faulty version of a pigment-synthesizing enzyme How do we get from DNA to traits??? - ansGene Expression: DNA directs of synthesis of proteins What are the two steps of getting from DNA to Traits? - ans1. Transcription
  5. Translation How do we get from a sequence of nucleotides to a trait in the organism? - ansThe Central Dogma What is the Central Dogma? - ansA cellular chain of command. DNA ---> RNA ---> Protein
  6. DNA = A polymer of nucleotides in a specific order
  7. RNA = A process of going from one "language" to another in 2 fundamental steps
  8. Protein = A polymer of amino acids in a specific order When a single pre-mRNA molecule is made in one of your cells, how much information does it contain?

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A. The genetic code for all of the alleles from one chromosome B. The genetic code for one allele from one chromosome C. The genetic code for both alleles you have of that gene - ansB. The genetic code for one allele from one chromosome Which cells in the albino deer have the albino mutation? A. Only one cell B. All of its cells C. Only its gametes D. Only its cells that grow hair E. Half of its cells - ansB. All All of the cells in your body have basically the same genome Successful transcription requires 3 basic processes: - ans1. Initiation

  1. Elongation
  2. Termination Initiation:
  3. Figure out where to start reading DNA
  4. Actually begin making mRNA HOW???? We need a signal... - ansPromoter= the "start here" signal What does RNA Polymerase do during transcription? - ansUntwists DNA, makes RNA Summary of elongation in transcription: - ans1. RNA polymerase untwists and separates 10- 20 base pairs of DNA at a time
  5. This opens space for RNA nucleotides to come into the site of mRNA synthesis----> base pairing of RNA nucleotides with the DNA template
  6. RNA polymerase catalyes the addition of correct nucleotides onto the 3' end of the RNA molecule
  7. RNA polymerase moves along, the new RNA molecule peels away from the DNA, and the helix re-twists Before RNA transcripts leave the nucleus, they are modified in what way? - ans1. Alteration of ends
  8. Cutting out some of the middle (RNA splicing) This offers the cell a way of controlling when and where protein is produced Why do introns exist? - ans- Alternative splicing---> alternative mRNA---> multiple proteins from a single DNA sequnce
  • The possibility of "exon shuffling" may facilitate evolutionary change What are the nucleotides in RNA? - ansAdenine, Uracil (replaces thymine), Guanine, and Cytosine What is a codon? - ansA sequence of 3 nucleotides = specification of amino acid. Each triplet is called a codon Codons are read in what direction? - ans5' to 3'

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**Cannot be C because viruses don't have cells How does a virus do its business? - ans1. Virus enters the cell

  1. Cell manufactures viral proteins and copies of virus's genome
  2. Virus makes use of the host enzymes, ribosomes, tRNA, amino acids, ATP, etc.
  3. Assembly of new viruses
  4. New virus particles break out of the cell Two key variables used to classify viruses that infect animals: - ans1. DNA or RNA?
  5. Single or double stranded? How do viruses make you sick? - ans1. Damage or kill cells (with enzymes)
  6. Cause infected cells to produce toxins
  7. Envelope proteins that are toxic ****However, most symptoms & damage come from your own immune system!!! Apoptosis, inflammation, etc. Why can't we take antibiotics to successfully cure ourselves of viral infections? A. Viruses evolve resistance too quickly B. Viruses use our own cellular machinery C. Viruses are smarter than bacteria D. Viruses get inside our cells E Viruses replicate too quickly - ansB. Viruses use our own cellular machinery Why are vaccines effective in preventing viral illnesses? A. Vaccines are just like antibiotics B. Vaccines teach your immune system how to recognize specific viruses C. Vaccines make it impossible for a virus to enter your body D Vaccines change your cells so that no virus can enter them E. Vaccines add "virus-fighting" genes to your genome - ansB. Vaccines teach your immune system how to recognize specific viruses Why don't all influenza A viruses readily jump back and forth between humans, birds, pigs, dogs, etc? - ansMost viruses are host specific; specializing to infect one species can make a virus unable to infect another How do different viruses get mixed up? - ansReassortment: possible when multiple virus types infect the same individual host and get into the same cell.... think "viral sex" What are vaccines? - ansDerivatives of pathogenic microbes that stimulate the immune system to mount defenses against the actual microbe A mutation in DNA which causes a change in one letter of a codon could: A. have no effect B. change one amino acid in a protein C. cause a protein to have fewer amino acids than normal D. cause a protein to have more amino acids than normal E. all of the above - ansE. All Suppose a mutation in DNA cause a stop codon to appear prematurely in an mRNA transcript. What effect would this have? A. Cause a shortened version of the protein to be produced

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B. No effect C. Cause a different amino acid to appear in the protein at the location corresponding to this codon D. Cause a longer version of the protein to be produced - ansA. Shortened version How do DNA mutations alter proteins? - ans1. Substitution: Switching one nucleotide for another

  • May or may not cause a change in the protein
  1. Insertions or Deletions: Causes a frameshift
  • Almost always results in a shortened protein being made DNA template strand: 3' G T A C G G C C A T T G C 5' The mRNA transcript from this sequence would be: A.3' G T A C G G C C A T T G C 5' B.5' G U A C G G C C A U U G C 3' C.5' C A U G C C G G U A A C G 3' D.3' C A U G C C G G U A A C G 5' - ansC. Read 5' to 3' Complimentary base pairing How do we know what "reading frame" to use/ Where to start translating the mRNA and how to break it into codons? - ansSome kind of "start" signal in the mRNA sequence.... The first codon (AUG) establishes the start point and the reading frame Which of the following types of cells in your body has a gene for eye color? A. Eye cells only B. Toe cells C. Gametes only D. Brain cells E. All of the above - ansE. All cells are pretty much the same! What would need to happen in order for a healthy adult human to make more insulin? A. "Turn on" expression of the insulin gene in cells in the pancreas B. Activate pancreatic stem cells to differentiate to make more pancreatic cells C. "Turn on" the human insulin operon - ansA. "Turn on" expression of the insulin gene in cells in the pancreas ** Can't be C because only bacteria have operons Given what you know about eukaryotic gene regulation: To turn on expression of the insulin gene in pancreatic cells, what would be needed? A. A signal directly from the brain to each cell in the pancreas B. The sight, smell, and taste of a donut C. The right combination of transcription factors to promote expression of the insulin gene D. A mutation in the insulin gene - ansC. The right combination of transcription factors to promote expression of the insulin gene

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Cells in two regions secrete proteins (inducers) providing positional information: - ans1. ZPA (anterior-posterior)

  1. AER (if AER cells are removed, there will be no limb outgrowth) If ZPA was experimentally removed from a developing chick embryo's limb, what would happen? A. The limb would develop normally B. The limb would cease development C. The limb would develop with two thumbs D. The limb would develop with two pinkies - ansC. Two thumbs ZPA is on the pinky side.... without it, we will get whats on the opposite side What is gel electrophoresis? - ans- A method of separating and visualizing molecules of different sizes
  • A gel is used as a molecular sieve to separate molecules of different sizes
  • A current is applied that causes electrically charged molecules to move through the gel (DNA is negatively charged)
  • The DNA molecules can be "labeled" with radioactive or fluorescent parts for the purposes of visualization Dideoxyribonucleotides terminate DNA synthesis because they... A. Do not properly form complementary base pairs B. Can not be incorporated by DNA polymerase C. Lack a 3'=OH group preventing additional nucleotides from being added D. Cause the breakdown of the template strand - ansC. Lack a 3'=OH group preventing additional nucleotides from being added How is evolution defined? - ansChange over time in a populations genetic makeup Descent with modification Evolution refers to both patterns and processes. How do they differ? - ans1. Patterns
  • Observations of the natural world
  • Factual descriptions of change
  1. Processes
  • Mechanisms that produce a pattern How do hypotheses, theories, and facts differ? - ans1. Hypothesis: a testable, falsifiable explanation for a phenomenon of interest
  1. Theory: A framework of internally consistent ideas used for generating hypotheses
  2. Fact: A piece of information about circumstances that exist or events that have occurred; an actual occurrence Evolution is a... A. Fact B. Theory C. Hypothesis - ansFACT!!!

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  • Modern forms are not present in the early fossil record
  • Novel flu viruses arise
  • Populations of many species have gone extinct in our lifetimes Evolution refers to both patterns and processes. Is natural selection a pattern on a process? - ansA process.... Natural selection is one of multiple possible mechanisms that produce a pattern What best describes the relationship between evolution and natural selection? - ansEvolution refers to patterns and processes of descent with modification Natural selection is a mechanism that can produce the processes of evolution Darwin developed two main ideas: - ans1. Descent with modification explains life's unity & diversity
  1. Natural selection is a cause of adaptive evolution How does natural selection work? - ans1. Offspring are overly abundant
  2. .... Yet many populations remain stable in size
  3. Resources (food, mates) are limited.... only a subset of all individuals will survive & reproduce
  4. No two individuals are alike
  5. Variation is heritable.... individuals that happen to have traits conferring survival and reproductive advantage will tend to leave more offspring... such favorable traits will tend to increase in frequency in a population over time Which of the following is NOT a part of the theory of natural selection? A. More offspring are born than can survive and reproduce B. Resources essential for survival and reproduction are limited C. Heritable, phenotypic variation D. Offspring inherit characteristics from their parents that the parents acquire during their lifetimes E. New traits that are more adaptive than old traits can replace the old traits in a population - ansD. Acquired traits are not inherited What does natural selection most generally favor? A. having many offspring B. being large C. long life D. traits that benefit the species as a whole E. cooperative behavior - ansA. Having many offspring NOT for the survival of the individual or the survival of the species