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“Enzymes” Lab Worksheet (M/C #4) NAME:
Ex. 4-1: EXPERIMENTAL METHOD AND THE ACTION OF CATECHOL OXIDASE
What question are you addressing in this experiment?
Propose a testable hypothesis for the presence or absence of catechol oxidase in potato extract.
Predict the result of this experiment (if/then).
What is the purpose of each of the three test tubes?
1 – 2 – 3 –
Which is the control tube (think in terms of the independent variable)?
Why is a second control tube necessary?
Design a table below in which you will record your results:
Explain your results in terms of your hypothesis.
Ex. 4-2: INHIBITION OF CATECHOL OXIDASE
Propose a question about the catechol oxidase inhibitor PTU.
Hypothesize about the nature of inhibition by PTU (i.e., competitive or non-competitive).
Predict the results of the experiment based on your hypothesis (if/then).
Which test tube is the control?
Why was the concentration of the catechol increased in test tube 2?
It is best to add the potato extract to the test tubes last. Why is this?
Design a table and record your results below:
Explain your results in terms of your hypothesis.
One member of your team is not convinced you have adequately tested your hypothesis. How could you expand this experiment to provide additional evidence to strengthen your conclusion?
Ex. 4-3: INFLUENCE OF CONCENTRATION, pH, AND TEMPERATURE ON THE ACTIVITY OF AMYLASE
Experiment A: The Influence of Enzyme Concentration on the Rate of Starch Digestion
Formulate a question about enzyme concentration and reaction rate.
Hypothesize about the effect of changing enzyme concentration on the time of starch disappearance.
Predict the results of the experiment based on your hypothesis (if/then).
Time of starch disappearance in different concentrations of the enzyme amylase (do not exceed 4 min):
Tube % Amylase Time of Starch Disappearance (sec) 1 2 3 4 5
The independent variable is __________________________ and should be plotted on the _______ axis.
The dependent variable is __________________________ and should be plotted on the _______ axis.
Explain your results in terms of your hypothesis.
Experiment C: The Effect of Temperature on Amylase Activity
Formulate a question about temperature and reaction rate.
Hypothesize about the time of starch disappearance at various temperatures.
Predict the results of the experiment based on your hypothesis (if/then).
Time of starch disappearance in different temperatures for the enzyme amylase (do not exceed 4 min):
Tube Temp. Time of Starch Disappearance (min) 1 80 oC 2 37 oC 3 22 oC 4 4 oC
The independent variable is __________________________ and should be plotted on the _______ axis.
The dependent variable is __________________________ and should be plotted on the _______ axis.
Explain your results in terms of your hypothesis.
What do you think would happen to reaction rate in the tube incubated in ice if this tube, with enzyme and substrate already mixed, were placed in the 37oC water bath? ( explain your answer in terms of the effect of various temperatures on enzyme structure and the rate of enzyme activity )
