Docsity
Log inSign up
Docsity
Prepare for your exams
Prepare for your exams

Study with the several resources on Docsity

Earn points to download
Earn points to download

Earn points by helping other students or get them with a premium plan

Guidelines and tips
Guidelines and tips
Sell on Docsity
Sell on Docsity
Log inSign up

Prepare for your exams

Study with the several resources on Docsity

Find documents

Prepare for your exams with the study notes shared by other students like you on Docsity

Search Store documents

The best documents sold by students who completed their studies

Search through all study resources
Docsity AINEW

Summarize your documents, ask them questions, convert them into quizzes and concept maps

Explore questions

Clear up your doubts by reading the answers to questions asked by your fellow students

Earn points to download

Earn points by helping other students or get them with a premium plan

Share documents
download point icon20 Points

For each uploaded document

Answer questions
download point icon5 Points

For each given answer (max 1 per day)

All the ways to get free points
Get points immediately

Choose a premium plan with all the points you need

Study Opportunities

Choose your next study program

Get in touch with the best universities in the world. Search through thousands of universities and official partners

Community

Ask the community

Ask the community for help and clear up your study doubts

University Rankings

Discover the best universities in your country according to Docsity users

Free resources

Our save-the-student-ebooks!

Download our free guides on studying techniques, anxiety management strategies, and thesis advice from Docsity tutors

From our blog

Exams and Study
Go to the blog

BI 381: Exam 1 - Spring 2008: Genetics and Molecular Biology - Prof. Allen C. Gathman, Exams of Molecular biology

Southeast Missouri State University (SEMO)Molecular biology
Prof. Allen C. Gathman

The instructions and questions for exam 1 of bi 381: genetics and molecular biology, held in spring 2008. The exam covers various topics such as dna structure, replication, and techniques used in molecular biology. Students are required to fill in missing bases in a dna molecule, propose experiments to test hypotheses, and explain the mechanisms of different problems in e. Coli replication.

Typology: Exams

Pre 2010

Uploaded on 08/08/2009

koofers-user-tzs
koofers-user-tzs 🇺🇸

10 documents

1 / 6

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
BI 381: EXAM 1–
Spring 2008
Instructions:
a. Relax. b. Write your name on the back of the last page ONLY.
1. a. (20 points) Fill in the rest of this two base-pair DNA molecule. Show covalent bonds as
lines, hydrogen bonds as dotted lines, double bonds as double lines, carbons in a ring as
corners, and other atoms as their atomic symbols. You need not show hydrogens unless they
are part of hydrogen bonds or have some other functional significance.
MARK THE 5' and 3' ends of EACH STRAND.
pf3
pf4
pf5

Partial preview of the text

Download BI 381: Exam 1 - Spring 2008: Genetics and Molecular Biology - Prof. Allen C. Gathman and more Exams Molecular biology in PDF only on Docsity!

BI 381: EXAM 1–

Spring 2008

Instructions: a. Relax. b. Write your name on the back of the last page ONLY.

  1. a. (20 points) Fill in the rest of this two base-pair DNA molecule. Show covalent bonds as lines, hydrogen bonds as dotted lines, double bonds as double lines, carbons in a ring as corners, and other atoms as their atomic symbols. You need not show hydrogens unless they are part of hydrogen bonds or have some other functional significance. MARK THE 5' and 3' ends of EACH STRAND.
  1. Avery, MacLeod, and McCarty were trying to find out what the genetic material (also known in this case as the "transforming principle") was. Griffith had hypothesized that the transforming principle was a polysaccharide. a. (15 points) Propose an experiment to test Griffith's hypothesis, assuming that you have a well-equipped modern lab (one with all sorts of pure enzymes available). Describe clearly what you would do and how you would detect the results. b. (5 points) What observable results would Griffith's hypothesis predict you would get when you do your experiment as described in part a?
  1. (5 points each) Explain how each of the following problems is solved in E. coli replication. In each case, specify the enzymes involved and explain what they do to solve the problem. a. Pol III needs a primer with a 3' end to start replication, but the DNA is circular. b. As the strands of the template DNA molecule are separated, other parts of the molecule become supercoiled. c. There is RNA in the newly synthesized polynucleotide strands. d. The lagging strand is in short segments, rather than a continuous polynucleotide strand.
  1. (5 points each) a. What's the difference between a Southern blot and a Northern blot? b. You email me and I send you some of the Mep1 gene from Coprinus cinereus. Is this a probe? If not, what does it need in order to be one? Explain. c. You know how big the human genome is. If you have 1 microgram of human genomic DNA, how many copies of the genome do you have? Show your work. d. When you do agarose gel electrophoresis, which DNA fragments wind up closest to the positive electrical terminal? Why?