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BI 200 - Exam #2A Name Fall 2003 Lab Section. Seat# Disclaimer Consider each question, and answer each in the appropriate format (e.g., multiple choice). You may qualify your answer if you have reservations. If your comments have merit, you may receive partial or full credit. Questions are 1 point each unless indicated. Multiple choice - 2 points each.
- The presence of membrane-enclosed organelles is a characteristic of A) prokaryotic cells. B) eukaryotic cells. C) all cells. D) viruses.
- Lactose enters E. coli via A) group translocation B) the phosphotransferase system C) a proton symporter called a permease D) facilitated diffusion
- The cell wall is A) interior to the cytoplasmic membrane. B) exterior to the cytoplasmic membrane. C) a part of the cell membrane. D) the same thing as the cytoplasmic membrane.
- Which is not true about the peptide interbridge structures of peptidoglycan? A) formation is prevented by lysozyme B) Gram positives like Staphylococcus aureus contain pentaglycine interbridges C) meso-diaminopimelic acid is present D) rare D-amino acids are present E) none of the above, all are true.
- Gram positive bacteria A) have a thick cell wall that is exposed to the environment. B) have a thick cell wall that is covered by the outer membrane C) have cell walls that contain the pyrogen lipopolysaccharide D) have cell walls that are flexible but excellent chemical barriers
BI 200 - Exam #2A Name Fall 2003 Lab Section. Seat# Disclaimer Consider each question, and answer each in the appropriate format (e.g., multiple choice). You may qualify your answer if you have reservations. If your comments have merit, you may receive partial or full credit. Questions are 1 point each unless indicated. Multiple choice - 2 points each.
- The presence of membrane-enclosed organelles is a characteristic of A) prokaryotic cells. B) eukaryotic cells. C) all cells. D) viruses.
- Lactose enters E. coli via A) a proton symporter called a permease B) the phosphotransferase system C) group translocation D) facilitated diffusion
- The cell wall is A) exterior to the cytoplasmic membrane. B) interior to the cytoplasmic membrane. C) a part of the cell membrane. D) the same thing as the cytoplasmic membrane.
- Which is not true about the peptide interbridge structures of peptidoglycan? A) formation is prevented by lysozyme B) Gram positives like Staphylococcus aureus contain pentaglycine interbridges C) meso-diaminopimelic acid is present D) rare D-amino acids are present E) none of the above, all are true.
- Gram positive bacteria A) have a thick cell wall that is exposed to the environment. B) have a thick cell wall that is covered by the outer membrane C) have cell walls that contain the pyrogen lipopolysaccharide D) have cell walls that are flexible but excellent chemical barriers
- The membrane of a gas vesicle is composed of A) various phospholipids. B) protein. C) carbohydrate. D) both glycoproteins and phospholipids.
- Endospores are A) bacterial reproductive structures. B) able to withstand extreme environmental assaults. C) metabolically active D) all of the above
- Which are not visible under a light microscope? A) bacterial flagella B) eukaryotic cilia C) eukaryotic flagella D) none of the above, all are >200 nm in diameter
- Maltose enters the cell with the help of three proteins: a periplasmic binding protein, a channel protein in the membrane, and an ATP kinase on the inside of the cell. A) Passive diffusion B) Primary active transport C) Secondary active transport D) Group translocation E) “ABC” transport
- Glucose is chemically altered upon entering the cell. This is an example of: A) Osmosis B) Diffusion C) Facilitated diffusion D) Active transport E) Group translocation
- Glycerol enters the cell by moving from high concentration to low concentration, and shows saturation kinetics. This is an example of: A) Osmosis B) Diffusion C) Facilitated diffusion D) Active transport E) Group translocation
Complete the following table comparing the arrangement of DNA in Eukaryotes and Prokaryotes. 5 points Eukaryotes Prokaryotes Shape of Chromosome Number of Chromosomes Copies of Each Chromosome Compartmentalization of Chromosome Supercoiling Involves Histones? Of the five Carbon atoms in ribose and deoxyribose, match the structural significance of each with the carbon number. 5 points _____ C1 A. Phosphate group is attached to this C _____ C2 B. No particular importance _____ C3 C. Is missing an O atom in deoxyribose _____ C4 D. Free hydroxyl is where next nucleotide is attached _____ C5 E. Nitrogenous base (A,G,C,T,U) attached here
Complete the following narrative by circling the word or phrase in each parentheses that most accurately completes the statement. (1 point each). Motility in prokaryotes takes a variety of forms including swimming, gliding, swarming, and floating. Swimming through liquid media involves flagella that are about (0.02 μm, 0.2 μm, 0.2 mm)m, 0.2 μm, 0.2 μm, 0.2 mm)m, 0.2 mm) thick, and are composed of many copies a single protein called (tubulin, flagellin, lysozyme). Flagellar motion in prokaryotes resembles that of a (whip, oar, propeller) and the energy source is (sunlight, μm, 0.2 μm, 0.2 mm)H+, ATP). Gliding is movement over a surface and is carried out bacteria such as (amoeba, cyanobacteria, Escerichia ) and ( Cytophaga , Paramecium , Enterococcus ). The former uses (slime, ratcheting proteins, peritrichous flagella) while the latter uses (slime, ratcheting proteins, lophotrichous flagella). Floating can be controlled in photosynthetic bacteria by structures called (hydrogenosomes, gas vesicles, chloroplasts). Draw a bacterium with peritrichous flagella and a ciliated protozoan. (2 points)
Fill in the blank/Short answer/Circle the correct word. 2 points each Give the name of the cell wall material in fungi, the name of the monomer it is composed of, and the designation of the bonding arrangement. Give the name of the cell wall material in algae, the name of the monomer it is composed of, and the designation of the bonding arrangement. In archaea the hydrophobic portion of the lipids are branched molecules called (sterols, phytanyls), which are made of (sterol, isoprene) subunits. Eukaryotic cells have (linear, circular) chromosomes that are composed of (single- stranded, double-stranded) DNA. Prokaryotic cells have (linear, circular) chromosomes that are composed of (single- stranded, double-stranded) DNA. How many base pairs in the E. coli chromosome? How many genes in the E. coli chromosome?
Give 4 lines of evidence that suggest that mitochondria and chloroplasts arose from endosymbiosis. (1 point each) A. B. C. D. Make a diagram of a chloroplast indicating the outer membrane, thylakoid membranes, grana, and stroma. Indicate the metabolic activities associated with the membranes and the stroma. 5 points.
BONUS
What are three advantages of having a capsule? Put a star by the most important. 4 points A - B - C - Write a joke here – 2 points
