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Fluorescent Assay: Popular Method for Cell Proliferation, Essays (university) of Health sciences
The fluorescent cell proliferation assay is a commonly used method in cell biology for determining cell proliferation. This non-invasive assay enables accurate counts of cells for various concentrations and experimental conditions, making it ideal for high-throughput screening and sensitivity studies. In this assay, cells are incubated with a non-fluorescent reagent that becomes bright red fluorescent when metabolically active live cells convert it. The intensity of the fluorescent signal indicates the number of viable cells, which can be used to measure the effects of compounds or agents on cell proliferation. This assay is applicable to both adherent and non-adherent cells and can be used in toxicology tests and drug screens.
What you will learn
- How can the Fluorescent Cell Proliferation Assay be used in toxicology tests and drug screens?
- How can the Fluorescent Cell Proliferation Assay be used in toxicology tests and drug screens?
- How can the Fluorescent Cell Proliferation Assay be used in toxicology tests and drug screens?
- How can the Fluorescent Cell Proliferation Assay be used in toxicology tests and drug screens?
- How can the Fluorescent Cell Proliferation Assay be used in toxicology tests and drug screens?
- How can the Fluorescent Cell Proliferation Assay be used in toxicology tests and drug screens?
- How can the Fluorescent Cell Proliferation Assay be used in toxicology tests and drug screens?
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2018/2019
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Fluorescent Cell Proliferation Assay
Cell proliferation assay plays a significant role in cytotoxicity and many other areas of cell biology. A number of methods have been developed for the determination of cell proliferation. Since Fluorescent Cell Proliferation Assay enables accurate counts of cells for a wide range of cell concentrations and experimental conditions, this assay have been the most widely used in the quantification of cell proliferation among all of these methods. High throughput screening and high sensitivity can also be achieved in the study of cell proliferation. In fluorescent cell proliferation assay, cells are incubated with the fluorometric reagent which could provide a fluorometric format to determine and monitor cell proliferation for a proper time. This cell proliferation reagent is non- fluorescent and can be converted into a bright red fluorescent form by the metabolically active live cells. When cells are treated with compounds or agents that can influence cell proliferation, viable cells can be quantified by measuring the intensity of fluorescent signal. If cell proliferation is increased, there are more viable cells which can convert more fluorometric reagent to fluorescent format and there will be a high intensity of fluorescent signal. While a decrease in cell proliferation causes a low intensity of fluorescent signal, which indicates the toxic effects to the cells. This assay can be applied widely including adherent and non-adherent cells. This assay also can be used in toxicology test and the efficacy study of therapeutic chemicals drug screens.
The fluorescent cell proliferation assay is convenient and versatile for the studies of cell proliferation. With many distinct advantages, it can lead to a further understanding of cell proliferation mechanisms and cell-mediated cytotoxicity.