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Student Name Alissa Obryan Student ID 271304 Lesson Laboratory Safety Institution Coastal Carolina Community College Session 275-02W Summer 2023 Course Microbiology 275-02W Summer 2023 Instructor Marie Smith
Final Report
Test Your Knowledge
Microbiology 275-02W Summer 2023
Laboratory Safety
Tightly woven fabric used to smother and extinguish a fire
Consists of absorbent material that can be ringed around a chemical spill until the spill can be neutralized
Device used to control small fires in an emergency situation
Provides chemical, physical, health, and safety information regarding chemical reagents and supplies
Area containing an exhaust fan to expel noxious fumes from a laboratory
Match each term with the best description.
Fire blanket
Spill containment kit
Fire extinguisher
Safety Data Sheet
Fume hood
1
2
3
4
5
Label each image with the correct term.
1 Safety shower^2 Eye-wash station 3 First aid kit
4 Burner fuel 5 Fire extinguisher
To better protect the body from chemical spills, wear _____.
long-sleeved shirts
full-length pants
closed-toe shoes
All of the above
At home, a _____ is the best substitute for a laboratory safety shower.
water can
bottle of water
kitchen faucet
bathroom shower
A Safety Data Sheet contains information about first-aid procedures, required protective equipment, and health effects.
True
False
After using loops, swabs, toothpicks, spreaders or any other equipment to transfer cultures, immerse the contaminated equipment in ______.
pure bleach
a strong acid
tap water
All of the above
Burns can occur when skin comes in contact with _____.
acids
bases
acids and bases
Exercise 1
Data Table 1: Lab Safety Equipment Alternatives
Exercise 2
Data Table 2: Sodium Hypochlorite SDS information
Most pieces of glassware are designed to be heated.
True
False
To test the smell of a substance, partially fill your lungs with air and, while standing slightly back from the fumes, use your hand to waft the odors gently toward your nose.
True
False
If any chemical in the kit has been ingested, you should _____.
call the Poison Control Center
seek a physician
both call the Poison Control Center and seek a physician
Shower or Sink Paper Towels Well-Ventilated Area
No Substitution
laboratory shower spill containment kit
fume hood first aid kit
eyewash station fire extinguisher
safety goggles
Exercise 3
Safe Disposal
Environmental/ Ecotoxicity
Spill Cleanup Procedures
Store in a tightly closed container and in a cool, dry, well- ventilated area away from incompatible substances.
Bleach can be poured down the sink by small amounts as long as the bleach is diluted by the running water.
no data/information is available
absorb spill using an absorbent, noncombustible material such as earth, sand, or vermiculite. Also provide ventilation.
very small quantities of chemicals to protect ones safety during experiments at home.
What are small-scale techniques?
- Protect work table/surface by covering it with plastic sheeting or a white garbage bag
- Have an open window or stove exhaust fan to provide proper ventilation
- Have accessibility to water nearby (sink, bottled water, or bathroom shower)
- Have a first aid kit nearby
- Keep sodium bicarbonate (baking soda) on hand to neutralize spilled assets because the concentrated acids can cause serious burns
List 5 precautions that must be taken before beginning an experiment.
Exercise 4
Data Table 3: Part 1 and Part 2 of the Safety Contract
Sodium bicarbonate (baking soda)
As indicated in the video, what common substance can be used to neutralize a spilled acid?
used chemical containers can be contaminated and an unexpected and potentially unpleasant reaction can occur.
According to the video, why should a used chemical container never be refilled?
Two examples of heat-sensitive glassware are graduated cylinders and flasks, and can shatter when exposed to heat source.
Certain glass objects are not meant to be heated and could shatter if exposed to a heat source. What two examples of heat-sensitive glassware are given in the video?
The _____ should be contacted immediately if you or anyone accidentally consumes or comes in contact with a potentially toxic substance.
National Chemical Toxicity Center
National Poison Control Center
American Poison Chemical Society
American Toxic Chemical Center
At the conclusion of experiments involving growing bacterial cultures, _____ should be added to the plates or tubes and everything should be soaked for _____.
pure acid; 1 hour
a base; 30 minutes
pure bleach; 2 hours
pure bleach; 15 minutes
When working with acids, always _____ to avoid chemical splattering.
add acid to water
add water to acid
Acid spills can be neutralized by adding _____.
water
baking soda
more acid
distilled water
All of the above
Graduated cylinders and volumetric flasks are designed to be heated.
True
False
A _____ can be used to douse a fire.
fire extinguisher
safety shower
fire blanket
fire blanket and fire extinguisher
All of the above
Chemicals may be returned to their containers after they have been dispensed.
True
False
Normal eyeglasses may be worn to substitute for safety goggles.
True
False
A _____ expels noxious fumes from a laboratory.
fire blanket
fume hood
spill containment kit
All of the above
A sink faucet or hand-held shower wand are appropriate substitutes for an eyewash station.
True
False
a. List the potential acute health effects. Eye: irreversible eye injury, irritation, and severe burns
Skin: severe burns and ulceration
Ingestion: severe digestive tract burns with abdominal pain, vomiting, and possible death. It could also cause corrosion and permanent tissue damage to the esophagus and digestive tract Inhalation: Severe irritation to the respiratory tract with symptoms like sore throat, coughing, SOB, delayed lung edema, and corrosive action to mucous membranes.
b. List the potential chronic health effects
Dermatitis, Erosion of the teeth, Bleeding of nose and gums, Chronic bronchitis, Chronic gastritis
c. Identify the first aid measures for ingestion.
First aid measures for ingestion: Rinse mouth firstly. Do NOT induce vomiting and large amounts of water need to be given.
d. record the flammability of the product
Flammability: non-flammable
e. Identify 3 chemicals that produce a potentially dangerous reaction with hydrochloric acid. Chemicals which can react dangerously with HCl: HCl can react with some metals forming flammable hydrogen gas, Hydrogen chloride may react with cyanide to form lethal concentrations of hydrocyanic acid and mixing with water can result in splattering and
Use the Internet to look up the SDS for hydrochloric acid and answer the following questions:
a. List the potential acute health effects.
b. List the potential chronic health effects.
c. Identify the first aid measures for ingestion.
d. Record the flammability of the product.
e. Identify 3 chemicals that produce a potentially dangerous reaction with hydrochloric acid.
f. Describe how to handle small spills.
g. List the personal protection when working with the chemical.
release of large amounts of heat.
f. Describe how to handle small spills. How to handle small spill: One should completely contain the spilled materials with dikes, sandbags, etc. The ventilation system should be shut off if needed. Reuse or reprocess where possible. Neutralization should be carried out with soda ash or dilute caustic soda.
g. List the personal protection when working with the chemical. Personal Protection: Gloves, Lab-coat should be worn, breathing vapor or mist should be avoided. Do not get in eyes, on skin, or on clothing as far as possible.
