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Student Name Alissa Obryan Student ID 271304 Lesson Microbiology Laboratory Preparation Institution Coastal Carolina Community College Session 275-02W Summer 2023 Course Microbiology 275-02W Summer 2023 Instructor Marie Smith
Final Report
Test Your Knowledge
Microbiology 275-02W Summer 2023
Microbiology Laboratory Preparation
True False
Identify each statement as true or false.
1
Exposure to bright light can inhibit microbial growth.
Goggles, gloves, and a face mask should be worn whenever working with active cultures.
Microbiology lessons often require incubation periods and active cultures.
2
Normal flora are pathogens typically found in or on the human body.
Most microbiology lessons can be completed in 1 or 2 hours.
Low incubation temperatures decrease the amount of time required for microbial growth.
Exploration
Microorganisms found in or on the human body on a semi-permanent basis without causing disease
Process of growing microbes in a controlled environment
Process of melting and adding agar to Petri dishes
Population of living microbes, in sufficient numbers, for use in experiments
Match each term to the best description.
Normal flora
Incubation
Plate pouring
Active culture
_____ is a solid gel used to grow microbes in the laboratory.
Agar
Broth
Normal flora
All of the above
Many microbiology lessons require _____.
plate pouring
active time
incubation periods
All of the above
1
2
3
4
Data Table 1: Time Allocations for Microbiology Lessons
When writing out all the experiments on my calendar, I put them in the weeks based on the syllabus. I can see that you do not have to do more than one experiment at a time to get all experiments done by the due date. Based on my calendar, it shows that you are able to get all experiments done before due dates while only doing one at a time. However, while doing agar plates, you can save yourself time by doing them all together at once and storing them.
Will you need to conduct more than one experiment at a time to meet the assignment due dates? Explain your answer by referencing the completed calendar.
Lesson Due Date
Lesson Active Culture
pre-lab lesson task- Lab kit inventory of materials and introductions discussion board
May 17, 2023 @11:59pm
Syllabus and Schedules Quiz - 275W
May 18, 2023 @11:59pm
Smartwork5: What is a microbe? and important discoveries
May 18, 2023 @11:59pm
Lecture Notes - From atoms to cells (AT)
May 18, 2023 @11:59pm
Getting started May 22, 2023 @11:59pm
Pre-Lab Task - Lab Safety
May 22, 2023 @11:59pm
Smartwork5: Ch 4 From Atoms to Cells
May 23, 2023 @11:59pm
Lecture Notes - Basic Concepts of Infections Disease (AT)
May 23, 2023 @11:59pm
Laboratory Safety May 24, 2023 @11:59pm
pre-lab lesson task- Microbiology Laboratory Preparation
May 24, 2023 @11:59pm
Microbiology Laboratory Preparation
May 31, 2023 @11:59pm
pre-lab lesson task- Using the V-scope
May 31, 2023 @11:59pm
Lesson Active Time
pre-lab lesson task- Lab kit inventory of materials and introductions discussion board
Syllabus and Schedules Quiz - 275W
Smartwork5: What is a microbe? and important discoveries
Lecture Notes - From atoms to cells (AT)
Getting started
Pre-Lab Task - Lab Safety
Smartwork5: Ch 4 From Atoms to Cells
Lecture Notes - Basic Concepts of Infections Disease (AT)
Laboratory Safety
pre-lab lesson task- Microbiology Laboratory Preparation
Microbiology Laboratory Preparation
pre-lab lesson task- Using the V-scope
Lesson Incubation
pre-lab lesson task- Lab kit inventory of materials and introductions discussion board
Syllabus and Schedules Quiz - 275W
Smartwork5: What is a microbe? and important discoveries
Lecture Notes - From atoms to cells (AT)
Getting started
Pre-Lab Task - Lab Safety
Smartwork5: Ch 4 From Atoms to Cells
Lecture Notes - Basic Concepts of Infections Disease (AT)
Laboratory Safety
pre-lab lesson task- Microbiology Laboratory Preparation
Microbiology Laboratory Preparation
pre-lab lesson task- Using the V-scope
Photo 1: Microbiology Assignment Calendar
pre-lab lesson task- Lab kit inventory of materials and introductions discussion board
Syllabus and Schedules Quiz - 275W
Smartwork5: What is a microbe? and important discoveries
Lecture Notes - From atoms to cells (AT)
Getting started
Pre-Lab Task - Lab Safety
Smartwork5: Ch 4 From Atoms to Cells
Lecture Notes - Basic Concepts of Infections Disease (AT)
Laboratory Safety
pre-lab lesson task- Microbiology Laboratory Preparation
Microbiology Laboratory Preparation
pre-lab lesson task- Using the V-scope
Exercise 2
Data Table 2: Incubation Site Characteristics
Because bacteria like warm places to grow. If the place is cooler, it will take longer to grow.
Why should the incubation temperature for culturing microbes remain above 20 ° C?
Because bacteria like warm places to grow. If the place is cooler, it will take longer to grow. Due to the optimum environment for microbes, I selected panel 1 as the ideal incubation site for culturing microbes because the environmental conditions such as temperature, humidity, and nutrients medium etc are optimum. Microbes need specific environmental conditions for their growth and development. If the environmental conditions are not suitable so it adversely affected the growth and development of microbes.
Explain why you selected the location recorded in Panel 1 as the ideal incubation site for culturing microbes.
Photo 2: Final Incubation Location
Kitchen cabinet location
Microbe Body Location
Escherichia coli
Staphylococcus epidermidis
Microbe Possible Benefits
Escherichia coli
Staphylococcus epidermidis
Microbe Potential Dangers
Escherichia coli
Staphylococcus epidermidis
Microbe Information Source
Escherichia coli
Staphylococcus epidermidis
Intestines (humans and animals)
Skin and mucous membranes.
aids digestion and protects us from other harmful microbes.
Crucial for development of immune cell subsets including eûector T cells and MAIT cells
Most E. coli strains are harmless, but some can cause serious food poisoning.
can cause infections from implantation of medical devices, orthopedic devices, and CNS shunt.
It is transmitted to humans primarily through consumption of contamined foods, such as raw or undercooked ground meat products, raw milk, and contaminated raw vegetables and sprouts.
Data Table 4: Safety Scenarios
consisting of nonmotile, Gram- positive cocc, arranged in grape- like clusters. It forms white, raised, cohesive coloniesabout 1- diameter after overnight incubation, and is not hemolytic on blood agar.
Media provided in the lab kit should be stored in a(n) _____ upon arrival.
autoclave
refrigerator
incubator
None of the above
Most microbiology lessons require an average time of _____ to complete.
20 minutes
3 hours
5 days
None of the above
Incubation temperatures should remain between _____ C for cultures to
develop within 1 to 3 days.
o
10-
21-
35-
None of the above
Normal flora frequently cause disease to healthy individuals.
True
False
Individuals with compromised immune systems should consult their physicians before working with cultures.
True
False
Extension Questions
It may be necessary to work on more than one lesson at a time to successfully meet microbiology assignment due dates.
True
False
A(n) _____ is an example of an appropriate incubation site for culturing microbes.
exposed windowsill
unheated basement closet
empty laundry cabinet
All of the above
Escherichia coli resides in the _____ of healthy individuals.
large intestines
lungs
nasal passages
All of the above
One should always _____ before working with microbes.
put on protective gear
wipe down the work surface with alcohol or a bleach solution
wash their hands and forearms
All of the above
