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Student Name Alissa Obryan Student ID 271304 Lesson Microscopy for Microbiology – Use and Function Institution Coastal Carolina Community College Session 275-02W Summer 2023 Course Microbiology 275-02W Summer 2023 Instructor Marie Smith
Final Report
Test Your Knowledge
Eye piece that is typically 10X or 15X magnification
Calculated by multiplying the magnification of the ocular lens with the objective lens
Visible area of a slide that can be observed
Distance range that is in focus at a given time
Held by the turret closest to the stage, and range in power from 4x to 100x magnification
Microbiology 275-02W Summer 2023
Microscopy for Microbiology – Use and Function
1. Match the term with the definition.
Ocular lens
Total magnification factor
Field of view
Depth of field
Objective lens
1
2
3
4
5
Exploration
2. Label the parts of the microscope.
A compound microscope is a type of optical microscope.
True
False
The _____ sits below the stage and varies the field for viewing the specimen.
diaphragm
coarse adjustment knob
base
None of the above
1 Ocular lens
2 Arm 3 Objective lens 4 Coarse focus knob 5 Stage
6 Illuminator
7 Fine focus knob
8 Base
Letter Component Name Component Function
A B C D E F G H ocular lens each individual light ray traces a path towards a position which is opposite of what the orginal location is, which creates a mirrored image. This is similar to when you are looking in the mirror as what you see is opposite directions of what others see.
arm supports the lenses of the microscope itself which is connected to the base.
turet lenses can be rotated for selection of an appropiate magnification.
objective lens there are 3- objective lenses that range in different magnification powers. The V-scope provides me with 3 different magnification powers being 4x, 10x, and 40x.
condenser lens focuses light rays directe from the lgiht source (illuminator) onto the slide.
stage the flat surface on the microscope where you put slides on and the slide clips hold the slide in place for viewing of what is on the slide.
Diaphragm adjustment
varies the diameter of the light column.
Illuminator the light source which illuminates what is on the slide you are viewing.
Panel 1: Microscope Description
Data Table 2: Total Magnification
I
J
K
L
M
coarse focus knob used for larger amounts of focus as it moves the stage either down (farther away from your view) or up (closer to your view).
fine focus knob used for higher amounts of magnification almost as if you are zooming in on the object of what you are looking at.
ruler adjuster keeps the slide aligned with the ruler and moves the ruler.
ruler can measure the slide.
base the bottom of the microscope and like all bases, is a support or "foundation" for what it is holding. In this case the base is supporting letters A-L so we can properly view microbes and other cells.
I am using the SI V-Scope which has ocular lenses, arm, base, turet, objective lenses, coarse focus knob, fine focus knob, stage adjustment knobs, illuminator, diaphragm adjustment, condenser lens, stage, and a stage clip.
Photo 1: Letter e - Low Power
Lens Observations
Low Power
Medium Power
High Power
Oil Immersion
When using the low power, the entire "e" is visible and extremly clear.
I can see the grain and spotted background behind the letter "e" and some of the "e" itself.
I am able to make out that the black area of the slide is a zoomed in part of the "e" and the edges are blurred almost as if I were to take a permanen marker and press down hard on a piece a paper and seeing the outside of dot spreading ink.
The V-scope does not provide oil immersion lens.
Photo 2: Letter e - Medium Power
Photo 3: Letter e - High Power
Data Table 5: Prepared Slide Magnifications
Photo 5: Amoeba Slide
Using the formula "field of view/estimated number of cells that cross the diameter = Length of one cell. I estimated around 300 cells that would cross the diameter making the equation: 1/300 = 0.00333333mm. the 1 is from counting 100 lines at a total magnification of 60x in the field of view to start with and multiplying it by 0.01mm. The 300 is my approximate count of what could fit in the field of view from left to right or my approximate diameter.
Using the field of view calculated in Exercise 1 for the high power lens, what is the approximate diameter of each of the cells in the Bacteria Coccus Form slide in Photo 10? Show your calculations.
I used my digital ruler on my computer over my downloaded photo from the HOL of Bacteria Coccus Form slide and counted each cell across the ruler but I made sure to count only to 100 lines as that was my field of view for measurement. I also noticed the different shapes and colors as well as texture (look of texture not touch) of each slide which helped me tell each slide apart.
Detail techniques you found helpful for focusing on the various slides in this exercise.
Slide Magnification
Amoeba
Penicillium
Yeast
Spirillum
Bacillus
Coccus
150x
600x
600x
600x
600x
600x
Photo 6: Penicillium Slide
Photo 7: Yeast Slide
Photo 10: Coccus Slide
Exercise 3
Data Table 6: Wet-Mount Viewing Results
Photo 11: Prepared Slide - Cheek Cell Smear
The difference between these two slides is that the color and visibility of the dental plaque are more visible and colorful than the cheek cell wet mount. Although you can still see the cheek cell wet mount, it has an invisibility look to it and looks more liquified than the dental plaque.
Describe the similarities and differences between the cheek cell wet mount and dental plaque wet mount.
It became easier by remembering the steps I needed to take to prepare the wet mount slide in the correct way. I told myself water then slide when it came to the distilled water and cover slip. The microscope and picture were last for each step so I remembered that my first thing to do was get a blank slide out.
How did the process of preparing wet-mount slides become easier as you prepared the second wet-mount slide of this exercise?
Slide Magnification
Cheek Cell Smear
Dental Tartar Smear
600x
600x
Photo 13: Prepared Slide - Dental Tartar Smear
Photo 14: Dental Tartar Smear
Competency Review
The main component of an optical microscope that is used to focus an image at a high power of magnification is the _____.
coarse adjustment knob
diaphragm
fine adjustment knob
None of the above
The slide is placed on the _____ of an optical microscope.
stage
turret
base
None of the above
Depth of field refers to the distance range that is in focus at a given time.
True
False
