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Immunology Virtual Lab Worksheet, Exercises of Immunology

Wilmington CollegeImmunology

biointerctive student worksheet on immunology lab

Typology: Exercises

2020/2021

Uploaded on 04/20/2021

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www.BioInteractive.org
Published January 2015
Page 1 of 6
Student Worksheet
Virtual Lab
The
Immunology Virtual Lab
IMMUNOLOGY VIRTUAL LAB WORKSHEET
INTRODUCTION
Go to http://www.hhmi.org/biointeractive/immunology-virtual-lab. Start the Virtual Lab and maximize the
screen if you wish. Answer the following questions in the spaces provided.
DIAGNOSIS
1. Where are antibodies found?
_____________________________________________________________________________________
_____________________________________________________________________________________
2. How can they be used in the laboratory?
_____________________________________________________________________________________
_____________________________________________________________________________________
3. What does ELISA stand for?
_____________________________________________________________________________________
_____________________________________________________________________________________
4. What are ELISA assays used for in labs?
_____________________________________________________________________________________
_____________________________________________________________________________________
5. What are the three important limitations of an ELISA? Explain each.
Limitation
Explanation
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Download Immunology Virtual Lab Worksheet and more Exercises Immunology in PDF only on Docsity!

www.BioInteractive.org

Published January 2015

Student Worksheet

The Immunology Virtual Lab

IMMUNOLOGY VIRTUAL LAB WORKSHEET

INTRODUCTION

Go to http://www.hhmi.org/biointeractive/immunology-virtual-lab. Start the Virtual Lab and maximize the screen if you wish. Answer the following questions in the spaces provided.

DIAGNOSIS

  1. Where are antibodies found?
  1. How can they be used in the laboratory?
  1. What does ELISA stand for?
  1. What are ELISA assays used for in labs?
  1. What are the three important limitations of an ELISA? Explain each.

Limitation Explanation

www.BioInteractive.org

Published January 2015

Student Worksheet

The Immunology Virtual Lab

BACKGROUND

  1. What test can be used to determine whether a patient has an infectious or autoimmune disease?
  1. What does a positive result indicate?
  1. The watery fluid of the blood is called ____________________.
  2. What is allowed to react with the target antigen?
  1. Detection is possible when _________________________________________________.
  2. Once isolated, the secondary antibody can be ____________________________________
  1. What is the signaling system?
  1. What happens when the appropriate chemical (substrate) is added?
  1. How is the test quantified?
  1. What does the amount of color reflect?

www.BioInteractive.org

Published January 2015

Student Worksheet

The Immunology Virtual Lab

c. What is the negative control? (Step 4)

d. Why is it necessary to have a positive and a negative control? (Step 4)

  1. Why incubate the plate in step 5?
  1. Next, in step 6, the plate is washed. Why wash the plate?
  1. In step 7, a secondary antibody is added. What is a secondary antibody? Please define.

a. What is the attached enzyme in this assay? (Step 7)

b. What is the specific substrate for HRP? What color does it produce? (Step 7)

  1. How can the yellow color be quantitatively measured? At what wavelength? (Step 10, in "why")

www.BioInteractive.org

Published January 2015

Student Worksheet

The Immunology Virtual Lab

  1. Record your results. Indicate on this page and on the computer which boxes turned color.

A B C + (positive) – (negative)

1:

1:

1:

  1. Did you complete the ELISA correctly? (Yes/No) __________

If yes, proceed to #12 and #14.

If no, proceed to #13 and #14.

  1. What do the results indicate about:

Patient A:

Patient B:

Patient C:

  1. Explain what you did wrong and what you will need to do next time. For more information, check your printable summary page. Did your incorrect procedure provide you any results? Explain what went wrong.