WALTERS STATE COMMUNITY COLLEGE
COURSE SYLLABUS
Course: Introductory to Microbiology Laboratory - BIOL 2231
Semester: Fall/Spring/Summer
Instructor(s):
Course Supervisor: Dr. Jeff T. Horner, Dean of Natural Science, Office NSCI 126
Contact: Phone: 423-585-6865, E-Mail: Jeff.Horner@ws.edu
Office Hours: Instructors’ Office Hours are posted on their office doors
FAX: 423-318-2762
Secretary: 423-585-6865 (Michele Mutter)
Required Textbooks:
Microbiology Laboratory Exercises, Pamela Fouche’, Ph.D. and Donald W. Lindsey, Ph.D.
A&P and Microbiology Laboratory Study Disc, 7th edition
Catalog Description:
Laboratory exercises designed to demonstrate the distribution, isolation, cultivation,
identification, and control of microorganisms. The use of microorganisms in biotechnology will
be examined.
3 hour laboratory...........................................................................................................1 hour credit
Competencies and Objectives:
The student will be expected to:
1. Use proper sterile technique for handling microorganisms.
2. Learn the parts and correct method for usage of a standard laboratory compound light
microscope equipped with an oil immersion objective.
3. Use proper techniques for preparing and heat fixing a smear and staining bacteria.
4. Carry out and interpret simple, differential and special stains.
5. Compare and contrast the cell walls of gram-positive and gram negative bacteria.
6. Understand and interpret various biochemical tests for identifying bacteria.
7. Identify various protozoa, fungi, and extracellular parasites and list the diseases which
they cause.
8. Define the key terms related to the destruction or suppression of microbial growth:
sterilization, disinfection, antisepsis, germicide, bacteriostasis, asepsis, degerming.
9. Describe and demonstrate the various methods used for control of microbial growth and
evaluation of disinfectants and antibiotics.
10. Understand the theory behind and carry out the basic techniques used in immunology.
11. Know growth characteristics and tests for identifying various bacterial groups.
12. Carry out basic procedures used to determine water purity.
Instructional and Evaluation Methods:
Two laboratory exams worth 100 points each will be given. The exams will focus on activities
and results obtained from the laboratory exercises and assess the achievement of the learning
outcomes prescribed for this course. Your attendance is essential to your success. You are
