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Effect of Water pH on Pond Snails and Coffee Extraction, Lab Reports of Biology

Instructions for a lab experiment investigating the effect of water ph on the shell thickness of pond snails and the extraction rate of coffee. Students are asked to form a hypothesis and test the temperature, length of brewing time, or amount of water as independent variables. Procedures, materials lists, and suggested data analysis methods.

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2023/2024

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Biol 1100 Survey of Biology
Dr. Hattie Dambroski
Spring 2024
Lab #1: Investigating the Perfect Cup of Coffee
15 points
Learning Outcomes
1. Gain a working understanding of hypothesis formulation and testing.
2. Think about the design of scientific experiments. How do you design repeatable and experiments that clearly
test your hypotheses?
3. Create graphical representations of data.
4. Transmitting results to others in concise and specific terms.
Textbook
Read Chapter 1 in your textbook, for the best understanding and results.
Introduction
Biology is a science. Science is often misunderstood and mystified, however, it is merely a systematic way of
learning from one's own observations. The authority in science is the real world, not a textbook, professor, or
mystical Scientist. If your observations disagree with the textbook, then the text should be questioned. As such,
science is a dynamic field that is constantly questioning and challenging what we know. Often, what we know in
science is limited by technology, before the advent of the microscope not much was known about cells.
To learn by science, you must ask the right questions. Science can only study the natural world and assumes
that phenomena in nature have physical and tangible causes. As such, the existence of God is not testable by
scientific inquiry. However, you can ask the question, "Can lightning be explained as resulting from natural
forces?" (Rather than act of Thor, Zeus, etc.) You can ask the subsequent follow-up question, "Can lightning ever
be seen in the absence of the natural forces which have been shown to produce it?" After a sufficient chain of
questions, you can draw the inference that lightning is better explained as a natural weather phenomenon than as
an act of divinity.
Many science courses begin by asking students to memorize the steps of the scientific method. However, most
scientists would agree that there is no single method of solving scientific problems. One of the most popular
models of the scientific method includes the following steps:
1. Observation
2. Statement of the Problem/Question to be addressed
3. Formulation of hypothesis and prediction
4. Experimentation/ collection of data
5. Interpretation of data
6. Drawing conclusion
Although a scientist might not always follow these steps in sequence or may leave out or add some steps, a
scientific report of the investigation is usually presented in a manner similar to the above logical procedure. One
of the most important aspects of the scientific method comes after steps 1-6, where research is presented (usually
as an article) to other scientists for their review and scrutiny. The independent peer-review process is critical to
the scientific method as it holds scientists accountable. Additionally, science is not a static field, but a dynamic
and innovative field that is continually changing as we learn more and develop new technologies to study our
natural world.
Scientific studies often start with observations, but they are also important during the data collection phase of an
experiment. Observations are made with the senses. Sight is the major sense used in making observations, but
sounds, touch, smell, and even sometimes taste play significant roles. Scientists often use instrumentation to refine
their perceptions: thermometers, measuring devices for weight and distance, pH meters, light meters, and
microscopes are just a few that are commonly used by biologists.
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Biol 1100 Survey of Biology Dr. Hattie Dambroski Spring 2024

Lab #1: Investigating the Perfect Cup of Coffee

15 points Learning Outcomes

  1. Gain a working understanding of hypothesis formulation and testing.
  2. Think about the design of scientific experiments. How do you design repeatable and experiments that clearly test your hypotheses?
  3. Create graphical representations of data.
  4. Transmitting results to others in concise and specific terms. Textbook Read Chapter 1 in your textbook, for the best understanding and results. Introduction Biology is a science. Science is often misunderstood and mystified, however, it is merely a systematic way of learning from one's own observations. The authority in science is the real world , not a textbook, professor, or mystical Scientist. If your observations disagree with the textbook, then the text should be questioned. As such, science is a dynamic field that is constantly questioning and challenging what we know. Often, what we know in science is limited by technology, before the advent of the microscope not much was known about cells. To learn by science , you must ask the right questions. Science can only study the natural world and assumes that phenomena in nature have physical and tangible causes. As such, the existence of God is not testable by scientific inquiry. However, you can ask the question, "Can lightning be explained as resulting from natural forces?" (Rather than act of Thor, Zeus, etc.) You can ask the subsequent follow-up question, "Can lightning ever be seen in the absence of the natural forces which have been shown to produce it?" After a sufficient chain of questions, you can draw the inference that lightning is better explained as a natural weather phenomenon than as an act of divinity. Many science courses begin by asking students to memorize the steps of the scientific method. However, most scientists would agree that there is no single method of solving scientific problems. One of the most popular models of the scientific method includes the following steps:
    1. Observation
    2. Statement of the Problem/Question to be addressed
    3. Formulation of hypothesis and prediction
    4. Experimentation/ collection of data
    5. Interpretation of data
    6. Drawing conclusion Although a scientist might not always follow these steps in sequence or may leave out or add some steps, a scientific report of the investigation is usually presented in a manner similar to the above logical procedure. One of the most important aspects of the scientific method comes after steps 1-6, where research is presented (usually as an article) to other scientists for their review and scrutiny. The independent peer-review process is critical to the scientific method as it holds scientists accountable. Additionally, science is not a static field, but a dynamic and innovative field that is continually changing as we learn more and develop new technologies to study our natural world. Scientific studies often start with observations , but they are also important during the data collection phase of an experiment. Observations are made with the senses. Sight is the major sense used in making observations, but sounds, touch, smell, and even sometimes taste play significant roles. Scientists often use instrumentation to refine their perceptions: thermometers, measuring devices for weight and distance, pH meters, light meters, and microscopes are just a few that are commonly used by biologists.

Observations can be classified as qualitative or quantitative. Qualitative observations describe a general characteristic of an object or event: color, shape, texture, odor, taste, general weight/size, etc. Generalities resulting from simple qualitative observations are often too vague to be of much value. “The fish is fast, small, brown, and cute” may give us a general idea about the fish. More specific qualitative descriptions might tell us the species of fish, its reaction to objects in the environment, whether it is alone or in a school or other fish would be more informative. Quantitative observations involve careful measurement of amount, quantity, weight, speed, or size, and require accuracy. Quantitative observations are reported numerically, with a number associated with the observations and units (cm, g, etc.) when appropriate. Precise quantitative descriptions are desirable in scientific investigations. A statement of the problem involves asking a “how” or “what” question about the natural world. Most scientific problems center around “how” and “what” questions. What kinds of birds live around Normandale? How does temperature affect the extraction rate of green tea? How does the road salt, which is often toxic to plants, affect the growth of roadside grasses? “What” and “how” questions usually are answerable through experimentation and the collection of data. Which of the following are acceptable as statements of the problem?

  1. What is the effect of acid rain?
  2. What is the effect of acid rain on pond animals?
  3. What is the effect of water pH 4.2 on pond animals?
  4. What is the effect of water pH 4.2 on pond snails?
  5. What is the effect of water pH 4.2 on the shell thickness of pond snails? Statements 1-4 are too vague to be acceptable. The statements improve as they become more specific. Statement 5 is specific and not too long. Yet it contains two variables ; the variables are pH (acidity) and shell thickness of a specific snail species. The variable that is being studied (in this case, acidity) is called the independent variable , and this is the variable that the researcher will manipulate or alter to see what the effects are. The dependent variable (shell thickness here) is the variable the researcher is measuring. To say it another way, the independent variable is the variable that the researcher wants to see if it has an effect on the dependent variable. Once an acceptable statement of the problem has been formulated, a hypothesis can be proposed. A hypothesis is not a wild guess, but rather a plausible solution to the problem based on the investigator’s observations and previous knowledge or experience. If we assume that the hypothesis is correct, then a prediction can be made concerning future events. The hypothesis is often stated as one or two declarative sentences. The prediction is presented as an if... then... format. The “if” portion is the hypothesis. Let’s return to the snail questions, the problem stated in 5 above. Statement of problem/Question: What is the effect of water pH 4.2 on the shell thickness of pond snails? Hypothesis: Acid (pH 4.2) causes the snail to grow thinner shell. Prediction: If acid causes sails to grow thin shells, then snails cultured in water with pH of 4.2 will weigh less after 4 weeks (compared to controls or compared to original weight). Controls are always run side by side with experimental groups. Control groups are necessary to be sure your experiment is working correctly. If you do not run a positive control, a control you know will show a positive result, and the experiment gives no results, how can you be sure the results are truly negative? Controls are also needed to guard against experimental errors. There can be problems with reagents, which can degrade due to age or poor storage conditions, technicians can make mistakes by choosing the wrong reagents or making errors in dilutions or in pipetting, and/or experimental setup could have an effect on the experiment and cause results like the independent variable. In some cases, controls are also used as a comparison, so you have an idea of what would happen under non-experimental conditions. Controls are used to check the experimental set up so in the case of the coffee lab, you would want to test your experiment without adding coffee to your control - to make sure that nothing in the setup is changing the pH of

For all experiments use of pH stripes: Do not touch the end with the pad/slightly colored area. This is the end you will be putting into the water/coffee to check for the pH. Hold in solution for 5 secs and then compare right way with color chart for best identification of pH. A. Temperature of the Water

  1. Come up with a hypothesis concerning how the temperature of the solvent should affect the amount of material extracted. Write your hypothesis and the reasoning behind it in your lab report.
  2. Design an experiment to test this hypothesis. Test three different levels of your independent variable, that is, use three different temperatures of water. Remember that you must keep all other variables constant. The volume of water, time, and amount of coffee should not vary among treatments so that you can clearly see the effect of the temperature of water. What is your control? It is suggested you use a refrigerator or ice, room temperature, and boiling water for your three treatments and less than one tablespoon coffee. Remember to use degrees Celsius, not degrees Fahrenheit. And just for reference water freezes at 0C and boils at 100C, so you have to stay within those bounds. Room temperature is about 23C. Describe your plan in your lab report.
  3. Perform this experiment twice, and write both what you did and your results in your lab report. If anything unusual happened be sure to document it in the notes section of the data table. Were the results as you expected? If you failed to detect any differences among treatments, modify your experimental protocol and try again. Experiments which "didn't work" or give you unexpected results are often just as important as those that do.
  4. After your results have been recorded in the data tables, create a bar graph displaying the relationship between temperature of water and the average pH. You can draw by hand or use Microsoft Excel to create your graph. In one graph, the x-axis should be temperature of water and your y-axis will be pH. Be sure to include a title, axis labels, and a figure caption following the provided example in the Biology Guide to Making Figures. B. Length of Time
  5. Come up with a hypothesis concerning how the extraction time should affect the amount of material extracted. Write your hypothesis and the reasoning behind it in your lab report.
  6. Design an experiment to test this hypothesis. Test three different levels of your independent variable, that is, let the coffee grounds stay in the water for three different lengths of time. Remember that you must keep all other variables constant. The volume of water, temperature, and amount of coffee should not vary among treatments so that you can clearly see the effect of your treatment variable. What is your control? It is suggested you use hot water and less than one tablespoon coffee. Describe your plan in your report.
  7. Perform this experiment twice, and write both what you did and your results in your lab report. If anything unusual happened be sure to document it in the notes section of the data table. Were the results as you expected? If you failed to detect any differences among treatments, modify your experimental protocol and try again. Experiments which "didn't work" or give you unexpected results are often just as important as those that do.
  8. After your results have been recorded in the data tables, create a bar graph displaying the relationship between temperature of water and the average pH. You can draw by hand or use Microsoft Excel to create your graph. In one graph, the x-axis should be length of brewing time and your y-axis will be pH. Be sure to include a title, axis labels, and a figure caption following the provided example in the Biology Guide to Making Figures.

C. Volume of Water

  1. Come up with a hypothesis concerning how the extraction time should affect the amount of material extracted. Write your hypothesis and the reasoning behind it in your lab report.
  2. Design an experiment to test this hypothesis. Test three different levels of your independent variable, that is, use three different volumes of water to brew coffee. Remember that you must keep all other variables constant. The temperature, time, and amount of coffee should not vary among treatments so that you can clearly see the effect of you water volume. What is your control? It is suggested you use hot water and less than one tablespoon coffee. Describe your plan in your lab report.
  3. Perform this experiment twice, and write both what you did and your results in your lab report. If anything unusual happened be sure to document it in the notes section of the data table. Were the results as you expected? If you failed to detect any differences among treatments, modify your experimental protocol and try again. Experiments which "didn't work" or give you unexpected results are often just as important as those that do.
  4. After your results have been recorded in the data tables, create a bar graph displaying the relationship between temperature of water and the average pH. You can draw by hand or use Microsoft Excel to create your graph. In one graph, the x-axis should be volume of water and your y-axis will be pH. Be sure to include a title, axis labels, and a figure description following the provided example in the Biology Guide to Making Figures. For all experiments use of pH stripes: Do not touch the end with the pad/slightly colored area. This is the end you will be putting into the water/coffee to check for the pH. Hold in solution for 5 secs and then compare right way with color chart for best identification of pH.

Part 2: Data Collection & Analysis Record your results here What is your Independent Variable? What is your Control? Fill in the levels in the chart below. Trial 1. pH Color Notes Control: Level 1: Level 2: Level 3:** Trial 2. pH Color Notes Control: Level 1: Level 2: Level 3: ** Do not assume something is wrong with the experiment if the pH is the same for all of your levels, instead think about why that could be.** Amount of water 16.9 oz 3 tbl spoons 4 tbl spoons 5 tbl spoons 5ph light dark 6ph dark 5ph dark boiled for 5 mins. fresh filter used purified water thought this would have a darker color almost the same output to close to call still a dark roast but this doesnt surpise me I tasted this one after I took the ph it was a darker roast for sure almost burnt tasting but not 6 ph 3 tbl spoons 4 tbl spoons 5 tbl spoons 6 ph light 5ph medium dark medium dark burnt tasting. lower ph but still darker than 3 tbl spoons 5ph dark 2 tbl spoons 6 ph light not surprised it should get darker and keep a ph of 6 2 tbl spoons with 16 fl oz boiled for 5 mins 2 tbl spoons 6 ph light little darker than the first time but same concept almost the same as first trial dark and consistant with results

Average your results in the Table below and graph the pH results only. See question 8 below. Table 3: Average of your results. pH Control: Level 1: Level 2: Level 3: Post Lab Questions- Answers should be written in complete sentences. The graph you create for #8 below should be inserted into this report or uploaded by itself into D2L. See Biology Guide to Making Figures on the lab D2L webpage_._

  1. Explain how you ended up actually doing your experiment. Did you follow the design you came up with in

    6 on page 6 of the lab? What didn’t you consider in your initial experimental design?

  2. Graph the average pH results from Table 3. See Biology Guide to Making Figures on the lab D2L webpage_._ Your graph can be hand drawn or computer generated. Examining the bar graph that you made of the relationship between your independent variable and pH, do your results support or reject your hypothesis? Explain why or why not. Do you see any trends in your trials? Explain why you think you got the results you did.
  3. How does your independent variable influence coffee color? How does your independent variable cause this? Explain if your results do or do not support your hypothesis. See lab introduction for information on what is happening.
  4. Every experiment has potential sources of error. Any good experimenter will know the major sources of error both to aid in designing and performing the experiment and in interpreting the results. What are some sources of error in your experiment? How could your experimental design be improved?
  5. Are you satisfied with your results? Why? Either way, what would you do the next time you do this experiment to become more confident in your results? 2 tbl spoons 6ph 3 tbl spoons 5.5ph 4 tbl spoons 5.5ph 5 tbl spoons 5ph I replicated the same experiment back to back and I think that the boiling point was different after heating the pot over and over I think that the amount of water doesnt change the coffee color as much as the amount of coffee that is roasted. as well as which test it was because of the intial temp. of the pot. The same pot was used and that might have caused an error because from the start the pot was at a different temp. after every attempt. I think that if you used a different pot for each trial it would have been different. No I am not happy with my results because I think I conducted it wrong. I think if I thought of the pot I would have got a better answer instead I think that I messed with results which can throw off the entire experiment. I also would have used tap water instead of purified water because I think it keeps it's same ph consistantly