Caffeine Extraction: A Comprehensive Guide, Slides of Pharmacognosy

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EXTRACTION OF

CAFFEINE

Presented by: Mizanur F

Contents

 Introduction  Method of extraction  Identification test  Analysis  Utilization  (^) Storage  Reference

Methods of extraction

The powder tea leaves is extracted with boiling water the aqueous extract is filtered while hot. The warm extract is treated with lead acetate to precipitate tannins and filtered. The filtrate is treated with excess of dilute sulphuric acid to precipitate lead in the form of lead sulphate.

The filtrate is boiled with activated charcoal to remove colouring matter. Filtered to remove charcoal. The filtered decolourized solution is extracted with chloroform successively. Combined the chloroform extracts and evaporated on water bath to yield caffeine (white powder). It is recrystallized with alcohol [2, 4].

Analysis of caffeine



Analysis by TLC

Sample preparation : 1mg of Caffeine is dissolved in 1ml of methanol or Chloroform Standard sample :Caffeine Stationary phase : Silica gel-G Mobile phase: Ethyl acetate: methanol: acetic acid (80:10:10) Detecting agent: Expose to vapours of iodine RF Value: 0. Colour: Brown spot

Utilization

 Caffeine is a CNS stimulant  (^) Diuretic  It is used in beverage

Reference

1.Ansari SH. Essential of Pharmacognosy. 6 th ed. Birla Publication Pvt. Ltd. Delhi; 2013.

2. Khadabadi SS, Deore SL, Baviskar BA. Experimental Phytopharmacogsy. 2 nd ed. Nirali Prakashan, Pune; 2013.
3. Kokate CK, Purohit AP, Gokhale SB. Parmacognosy. 2 nd ed. Nirali Prakashan, Pune;

https://www.slideshare.net/USrinivasa/isolationide ntification-and-analysis-ofphytoconstituents .