1
Name ____________________________________ Lab Section ________
Isolation and Identification of Staphylococcus from the Nose and Skin Part II – (24 pts total)
Use the results for Isolate #1 in the document “RESULTS - Isolation and Identification of
Staphylococcus from the Nose and Skin” to complete the table and questions below.
Refer to Table A and the information in “Isolation and Identification of Staphylococcus
from the Nose and Skin – General Information and Procedures”.
“Isolate #1: Results and identification - 11 pts (0.5 pts each item in table)
Tests – Isolate #1
Results & Interpretation
(not just positive or negative,
what do the results tell you about
the unknown bacteria?)
Possible ID (Genus and species)
(From Table A: list all possible
Staphylococcus aureus,
Staphylococcus epidermidis,
Staphylococcus saprophyticus)
Morphology
Isolate #1
Gram stain result
Isolate #1
MSA plate – Growth?
Isolate #1
MSA plate –
Ferments mannitol?
Isolate #1
Blood agar
(type of hemolysis)
Isolate #1
Alexa Campanella
SU23
Cocci
It could be either of the three:
Staphylococcus aureus,
Staphylococcus epidermidis, or
Staphylococcus saprophyticus
Gram positive - purple cocci in
clusters
It could be either of the three:
Staphylococcus aureus,
Staphylococcus epidermidis, or
Staphylococcus saprophyticus
Good growth. This means the
organism is not inhibited by
NaCl.
It's a Staphylococcus species; It
could be either of the three:
Staphylococcus aureus,
Staphylococcus epidermidis, or
Staphylococcus saprophyticus
The media turns yellow around
growth, this means the
organism produces acid from
mannitol fermentation.
It could be Staphylococcus
aureus or possibly
Staphylococcus saprophyticus; it
can be positive or negative
Beta Hemolysis - Lysis of red blood
cells with complete destruction and
use of hemoglobin by the organism
resulting in a clear zone
surrounding the bacterial growth
It could be Staphylococcus
aureus
