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Lab 9 and 10 Unknown Lab report, Lab Reports of Microbiology

Clayton State UniversityMicrobiology
Prof. Renee E. Mcfarlane

This lab report shows the answers the the whole lab research in lab 9 and 10 Microbiology 2250L ..

Typology: Lab Reports

2022/2023

Uploaded on 11/25/2023

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Microbiology for the Health Sciences Laboratory (BIOL 2250L) Fall 2020
Lab 10: Unknown Lab
Special Lab: Unknown Lab Report Sheet
Name: __Abdulmalik Adesanya________________________________________
Unknown #: __2_____
1. Draw or take photo of the gram stain result of unknown bacterium.
Gram stain Reaction:
____Negative_____________
Morphology:
__Bacillus_______________
Chart 1
Biochemical Test Unknown Bacterium Result
Indole Negative
Methyl Red Negative
Voges Proskauer --- Blanked
Citrate Positive (blue)
Motility Positive
Glucose/Gas Positive; Positive (acid & gas)
1
pf3

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Lab 10: Unknown Lab

Special Lab: Unknown Lab Report Sheet

Name: __Abdulmalik Adesanya________________________________________ Unknown # : __2_____

  1. Draw or take photo of the gram stain result of unknown bacterium. Gram stain Reaction: ____Negative_____________ Morphology: __Bacillus_______________ Chart 1 Biochemical Test Unknown Bacterium Result Indole Negative Methyl Red Negative Voges Proskauer --- Blanked Citrate Positive (blue) Motility Positive Glucose/Gas Positive; Positive (acid & gas)

Urea Negative SIM Negative Catalase Positive (bubbles)

Chart 2 - Antibiotic Results

Antibiotic Zone of Inhibition Size

(mm)

Susceptible,

Intermediate or

Resistant

S 10

9mm R

Cz 30

0mm R

Te 30

0mm R

N 30

17mm I

AN 30

24mm S

GM 30

22mm S

  1. What is the identification of unknown bacterium? Klebsiella Pneumoniae
  2. What are the signs and symptoms of unknown bacterium that would occur? Describe the pathogenicity of the bacterium. Be detailed and thorough in your answer. Klebsiella pneumoniae is known for causing a specific type of pneumonia that often presents with sudden onset of high fever, chills, and productive cough with thick, blood- tinged sputum. It causes UTIs which involve frequent Urination, painful urination. Other symptoms could be fatigue and shortness of breath. The pathogenicity of Klebsiella pneumoniae includes Capsules, exopolysaccharides associated with mucoviscosity, lipopolysaccharides (LPSs), adhesins, and iron uptake systems.