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Lab 4 Blood Analysis Name __________________________ Date ____________________ Lab Section______________________
To Do Before Lab!
- Complete the lab reading below.
To Do After Lab!
- Keep all the notes and diagrams you create as part of the activities below. Lab practical exams will be composed of a selection of these questions/tasks.
Today’s Materials
- Compound light microscopes
- Prepared microscope slides
- Textbook
- Hematocrit samples
- Rulers
Table 26.2 Results of Pathological Differential WBC Count
Type of WBC Photo Observation Tally
% of Total WBCs
Class Average (% of Total WBCs)
Normal % of WBCs
Type of Disorder
Neutrophil
_____ x 100% = 100 60-70%
Lymphocyte
_____ x 100% = 100 20-25%
Monocyte
_____ x 100% = 100 3-8%
Eosinophil
_____ x 100% = 100 2-4%
Basophil
_____ x 100% = 100 0.5-1%
Total WBCs 100 100% N/A N/A N/A
Activity 2: Microscopy Practice
- Demonstrate appropriate recording of microscopic observations at 400x total magnification (TM): include labeling of the following boldface cells or cellular fragments.
- Your diagram need not be perfect! Make it plenty large so that you can include details. Use color for your sketches if possible. Ask the instructor for assistance if you’re uncertain about what you’re seeing!
Sickle Cell Anemia at 400x
- Sickled erythrocytes
- Normal erythrocytes
- Platelets (thrombocytes)
Activity 3: Blood Analysis
A hematocrit determines the volume of RBCs described as the presence of RBCs in a whole blood sample. A capillary tube of blood is centrifuged to pack the red cells at the outer end of the tube, separating them from the WBCs, platelets, and plasma. After measuring the length of the RBC column and the total length of the blood column, the percentage of RBCs can be calculated. The normal hematocrit range for females is approximately 38 to 46% and for males it is 40 to 54%. An abnormally high hematocrit (generally 65% or above) is indicative of polycythemia , and a hematocrit with RBCs below the normal level indicates a type of anemia.
- Interpret hematocrit values of four samples and indicate whether each is normal or suggests conditions, such as anemia, dehydration, or polycythemia.
- Patient A: __________________________________
- Patient B: __________________________________
- Patient C: __________________________________
- Patient D: __________________________________
