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This lab report is for students in General Biology or Bio I. This document is 7 pages long about The Effect of Temperature on Beet Membrane.
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Abstract The beet tissue is used to determine the effect temperature has on the cell membrane. Beet has betacyanin which is a pigment and the amount of betacyanin that is released from the cell will determine its permeability. This experiment was conducted by having 5 beet tissue of exact measurements that was placed in water baths of different temperatures. The solution resulting from the water bath was placed in new test tubes to record the absorbance using a spectrophotometer. The results showed that the temperature damages the cellular membrane of the beet and most of the betacyanin were released at a high temperature. Introduction Cellular membranes are made up of lipids and proteins whereas phospholipids are the most abundant lipids in membranes. A phospholipid is an amphipathic molecule that contains two distinct regions. The hydrophilic region is attracted to water whereas the hydrophobic region is repelled from water. The cell membrane is composed of a double-layered, phospholipid bilayer, in which proteins are embedded on the surface of the membrane. The purpose of the phospholipid bilayer is to prevent molecules from moving in and out of a cell, and, therefore, it is highly impermeable. Cell membranes are made up of proteins as well that move freely within the phospholipid bilayer due to its fluidity. The cellular membrane separates the contents within the cell from its outside environment (Urry et al., 2020). The permeability of a cell membrane increases as the temperature increases. At low temperatures, the phospholipids in a membrane are packed closely together leaving the membrane rigid. At higher temperatures, the phospholipids can move around which means that the membrane is permeable. The proteins begin to denature which also increases the cell membrane permeability (Urry et al., 2020). A beet tissue can be used in an experimental system
to test the effect of temperature on the membrane and to measure the cell membrane permeability. The beetroot has reddish pigments called betacyanin and when high temperature is applied to the beet cell membrane, the pigments can escape the cell leaving it permeable (Yang and Hinner, 2015). The purpose of this experiment is to determine how temperature affects the cell membrane of the beet tissue. If both high and low temperatures cause damage to cellular membranes, then the most betacyanin will be released from beet tissue exposed to high degrees Celsius. Methods A cork borer was used to cut beet cylindrically with a 5-mm inside diameter along with a 15 mm length. All the beet tissue was cut equally. The cylindrical beet was placed in a beaker to be rinsed for 2 minutes to wash off the betacyanin from the injured cells on the surface. Dispense the colored rinse-water. The beets were placed in five test tubes cautiously. The test tubes were labelled from 1-5 and the temperature treatment were written on each tube. Five test tubes were labeled with different temperatures (0, 20, 40, 60, 80) and 10mLs of distilled water were added to each test tube. The heated water baths were prepared for the corresponding temperature and the beets were placed in the water bath for 30 minutes. After the 30 minutes were up, the solution was then transferred into separate clean tubes with the corresponding label for the beet. The spectrophotometer was then calibrated using a test tube filled with distilled water that set the machine at an absorbance of 0nm. The spectrophotometer was used to measure the respective absorbance of the solutions (Vodopich & Moore, 2014).
betacyanin that is released into the water. Secondly, the temperature of the water should be accurate to do the experiment so that the absorbance value is not affected. Lastly, while using the spectrophotometer, it is important to calibrate it so that the absorbance values are not wrong which can ruin the results. To improve the accuracy of the experiment, it is necessary to have precise measurements of the beet tissue, using clean test tubes, label the test tubes, use the correct temperature, and calibrate the spectrophotometer. Thus, the conclusion is that the effect of temperature on the cellular membrane of the beet results in permeability. My hypothesis is supported by the data obtained from the spectrophotometer because it shows that the temperature damages the cellular membrane of the beet. During the experiment, most betacyanin were released at a high temperature. Works Cited Biology Practicals and Revision Biology Tutor. “Membrane Permeability Beetroot Practical.” YouTube , uploaded by Biology Practicals and Revision Biology Tutor, 27 May 2016. [accessed 2020 Oct 26]. www.youtube.com/watch?v=Hc3Mg0Yc7kI. Vodopich DS & Moore R (2014) Biology Laboratory Manual 10 th^ Edition_._ Exercise 10 Cellular Membranes Effects of Physical and Chemical Stress. McGraw-Hill NY. Urry, L., Cain, M., Wasserman, S., Minorsky, P., Orr, R. (2020). Campbell Biology (12). Pearson Education. ISBN: 9780135988046. Yang, Nicole J, and Marlon J Hinner. “Getting across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins.” Methods in Molecular Biology (Clifton, N.J.) , U.S. National Library of Medicine, 2015. [accessed 2020 Oct 26]. www.ncbi.nlm.nih.gov/pmc/articles/PMC4891184/.
Table 1. The absorbance is recorded in the table above for the beet solution using the spectrophotometer. Temperatures (x) Absorbance (y) 0 0. 20 0. 40 0. 60 0. 80 0. Table 2. The observation of the color intensity is recorded as a qualitative data whereas the absorbance values are quantitative data. Tube Number Treatment (Celsius) QUALITATIVE Data Color Intensity 0 = colorless to 10 = darkest red as most betacyanin released QUANTITATIVE Data Absorbance values 1 0 (beaker full of ice) 0 (no color) 0. 2…control sample 20 (room temperature/table top) 2 (very very light color) 0. 3 40 (water bath) 3 (light color) 0. 4 60 (water bath) 6 (medium color) 0. 5 80 (water bath*) 10 (darkest color) 0.