HEMATOCRIT, ESR, & EOFT
Hematology I
BS Medical Laborator
y
Science | SEM 1 2022
Hematocrit
●test that is performed based on
the principle of separating
cellular elements of blood from
the liquid part→ plasma
●“packed cell volume”
●defined as the percentage of
PCV to the total amount of blood
●provides useful information
about the size and content of
rbc when correlated with other
information i.e. rbc ct, hgb conc
●the numbers generated by true
tests enable to calculate rbc
indices
Methods of Hematocrit Determination
1. Macrohematocrit method
●known as the wintrobe method
●Wintrobe tube-special thick
walled hematocrit tube is used
●a long pasteur pipet is used to fill
its contents
●time consuming (seldom used)
formula:
hct % = prbc / total blood height x 100
ex. 42 / 95 x 100
= 44.2 or 44 %
44. 2 / 100 = 0.442 x 100 = 44
2. Microhematocrit method
●simple procedure: requiring 2-3
drops of blood wc makes an ideal
test to follow the prognosis of
anemic px or bleeding px
●special glass capillary tube is
used 1 mm in diameter & 7cm
long
●capillary blood/anticoagulated
venous blood
●if capillary blood- heparinized
ex. 42 % = 42 % or 42 / 100
= 0. 42
nv CU units SI units
male 41. 5-50. 4 % 0.415–0.504
female 35. 9–44. 6% 0.359–0.446
plasma: water, proteins, glucose,
hormones
buffy coat: leukocytes & platelets
hematocrit: red blood cells
note: the results are read at the top of
packed cell column
Hemoglobinometry
●Hemoglobin
- main oxygen carrying
pigment distributed in the
body
●heme- oxygen binding
pigment packed in protein
envelope→globin
●heme portion- consists of
porphyrin structure into which
Fe++ is suspended
- 98% iron exists in the
form of hemoglobin
1gm of hgb = 3.47 mg of Fe++
●globin portion- protein consists
of two pairs of AA chains
- contributes about 35% of
rbc, the rest is water
hgb nv:
male-14-17.5g/
dL
female- 12.3-15.
3 g/dL
SI: 140-175 g/L
123-153 g/L
