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Since the mid-1990s, techniques based around placing the sample into a vial containing a liquid medium that preserves the cells have been increasingly used. The media are primarily ethanol-based. Improvised method, can be used as a substitute. Works on different principle to ultimately give accurate results. Proper sample acquisition - a cell that is not in the sample cannot be evaluated.
Typology: Slides
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By Dr. Varughese George Department of Pathology. MGMCRI DR. VARUGHESE GEORGE
Basic principle :
****Ideal focus of sampling to obtain adequacy is Squamo-columnar Junction**
Specimen type Conventional PAP smear LBC Adequacy : Epithelial(8000- 12,000 cells well preserved and well visualised. Glandular components(10 well preserved cells in groups/single) For LBC - squamous cells) Glandular components(10 well preserved cells in groups/single) General categorization: Negative for intraepithelial lesion or malignancy[NILM] –organisms, other non neoplastic findings. Epithelial cell abnormalities – squamous cells, glandular cells.
Interpretation/result :
Reasons for Unsatisfactory Smear The following inhbits intepretation of 75 % of smear : -