MB (ASCP) 2020 with Comprehensive Questions
and Answers
45X genotype ✓Turner syndrome
5 nanomoles is how many micromoles? moles? picomoles? ✓5 nanomoles
* (0.001 micromole/1 nanomoles) = 0.005 micromoles
5 nanomoles * (10^-9 moles/1 nanomoles) = 5 *10^-9 moles
5 nanomoles * (1000 picomoles/ 1 nanomoles) = 5000 nanomoles
A chromosome without telomerase or terminal transferase would: ✓Get
shorter after every replication cycle
A difference in expression from maternal and paternal donated
chromosomes is ✓Imprinting
A List of PCR Components for PCR Reaction Mix Provided (Determine which
component is excess): ✓DNA Polymerase Concentration needs to be
reduced to 2.5-3 units - Taq Polymerase Conc was provided as 100 Units
as compared to other PCR Components
A paternity testing was performed in order to settle a paternity dispute,
where two men claim to be the father of a two-month old baby boy. Blood
samples from the baby, the mother and from the two potential fathers
were collected and sent to the Paternity Diagnostics Lab X for testing. STR
analysis was performed on 5 different loci (A1, B2, C3, D4 and E5) and the
results are shown below. (Please Note: PF1=Possible Father
1;PF2=Possible Father 2)
Locus Tested _Child _Mother_PF1_PF2
A1 _12/15_12_15/17_12/15
