MB ASCP EXAM 1 with Comprehensive Questions
and Answers
3' Exonuclease Activity ✓Proofreading-remove misincorporated nucleotide
by breaking the phosphodiester bond and replace it with the correct one.
5' Exonuclease Activity ✓Used to remove nucleotides when DNA pol III
encounters a nick, or discontinuity, thus moving the nick forward in an
activity called nick translation. Once the polymerase is dislodged, ligase
can close the DNA
Colicinogenic Factors ✓Carry resistance to bacteriocin, toxic proteins
manufactured by bacteria.
Conjugation ✓DNA moves cell to cell through physical contact. Bacteria
are of two sexes, F- and F+. F+ has a fertility factor that is responsible for
establishing a physical connection between mating bacteria.
Crossing Over ✓Physical exchange of molecules, how recombination
occurs.
DNA Methyltransferase ✓Catalyze the addition of methyl groups to
nitrogen bases, usually adenines and cytosines in DNA strands.
DNA Polymerase Alpha ✓Found in eukaryotes, involved in initiation of DNA
replication.
DNA Polymerase Beta ✓Found in eukaryotes, performs base excision
repair required for DNA maintenance and replication.
DNA Polymerase Gamma ✓The only polymerase found in mitochondria
