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(MB) ASCP Exam with Comprehensive Questions and Answers, Exams of Molecular biology

Villanova UniversityMolecular biology

(MB) ASCP Exam with Comprehensive Questions and Answers

Typology: Exams

2024/2025

Available from 04/04/2025

victor-kiragu
victor-kiragu 🇺🇸

211 documents

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(MB) ASCP Exam with Comprehensive Questions and
Answers
A sample was spiked with a positive control and results were negative for
positive control and the spiked sample. What happened? ✓Sample had an
inhibitor
Accuracy vs. Precision ✓Accuracy - refers to the closeness of a measured
value to a standard or known value
Precision - refers to the closeness of two or more measurements to each
other
Acute Promyelocytic leukemia translocation? ✓t(15,17)
Aldehyde dehydrogenase question ✓Alcohol dependence
Metabolizing alcohol
Antibiotic resistance mechanism in MRSA? ✓altered drug target
PBP2a
mecA
bDNA characteristics: ✓Uses alkaline phosphatase and amplifies the signal
Before a test can be implemented what metrics are required? ✓waived,
moderate complexity , high complexity
Carbamazepine (Tegretol) ✓Epilepsy
HLA-B 1502
pf3
pf4
pf5

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Download (MB) ASCP Exam with Comprehensive Questions and Answers and more Exams Molecular biology in PDF only on Docsity!

(MB) ASCP Exam with Comprehensive Questions and

Answers

A sample was spiked with a positive control and results were negative for positive control and the spiked sample. What happened? ✓Sample had an inhibitor Accuracy vs. Precision ✓Accuracy - refers to the closeness of a measured value to a standard or known value Precision - refers to the closeness of two or more measurements to each other Acute Promyelocytic leukemia translocation? ✓t(15,17) Aldehyde dehydrogenase question ✓Alcohol dependence Metabolizing alcohol Antibiotic resistance mechanism in MRSA? ✓altered drug target PBP2a mecA bDNA characteristics: ✓Uses alkaline phosphatase and amplifies the signal Before a test can be implemented what metrics are required? ✓waived, moderate complexity , high complexity Carbamazepine (Tegretol) ✓Epilepsy HLA-B 1502

Common form of DNA is: ✓B form, Right-Handed Cystic Fibrosis ✓3bp deletion on CFTR which causes it to be changed to Phenylalanine Denaturing breaks Inter-strand h bonds or intra-strand h bonds ✓Inter- strand Bonds (weaker) DNA repair polymerase(s): ✓Beta or Epsilon FLT3 is involved in what disease? ✓AML Formamide decreases Tm by: ✓altering H-bonds HCV genotype 1 vs 2 : Which has a low viral load and best sustained response to drugs? ✓HCV Genotype 2 Herceptin ✓Her2/NEU Highest vancomycin resistance? ✓vanA HIV viral load difference? ✓0.5 log 10 HLA-DR is on: ✓Exon 2 , Beta How long can blood be stored at 4degC for DNA analysis? ✓72 hours How long to keep obsolete document in lab according to CAP? ✓2 years

Order to add to a PCR reaction ✓Master mix Patient sample Positive control Negative control PCR Inhibitors ✓Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenal blue PPV ✓Positive predictive value (PPV): the proportion of people with positive test results that actually have the disease (i.e. are correctly diagnosed with the disease) Primer dimers are formed due to: ✓3' Complementarity RNA storage condition for 6 months? ✓-70C in Ethanol Sensitivity ✓ability of a test to correctly identify those with the disease (true positive rate) Sensitivity vs. Specificity ✓Sensitivity - the ability of a test to correctly identify those with the disease (true positive) Specificity - the ability of the test to correctly identify those without the disease (true negative) Specificity ✓ability of a test to correctly identify those without the disease (true negative rate) Start Codon ✓AUG (methionine)

Stop Codon ✓UAA, UAG, UGA Thermal devices are verified by which agency? ✓NIST To avoid RNAse at what temp should you bake the glassware? ✓250*C for 4 hours Topoisomerase vs Helicase ✓Topoisomerase - Unwinds Helicase - Separates Virus affecting patients after kidney transplant is ✓BK Virus Warfarin is associated with? ✓VKORC1 and CYP2C What amino acids compose histones? ✓Lysine and Arginine What enzyme binds the 5' end to 3' end of ds dna? ✓Primase What gel do you use for 200-250 bp? ✓6% Polyacrylamide Gel What is HPLC ✓High Performance Liquid Chromatography a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase) What is the effect of Irinotecan and UGT1A1? ✓Decrease drug metabolism What is the proper GC content of primer? ✓50-60%