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METHODS OF STERILIZATION, Cheat Sheet of Microbiology

Quick revision notes for med and microbiology students.

Typology: Cheat Sheet

2021/2022

Available from 08/19/2024

RheainMed
RheainMed 🇮🇳

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STERILIZATION
Physical
Chemical
Heat
based
Filtration
Radiation
HEAT
I
I
Dry
-
>
DEP
Moist
-
>
DL
·
Flaming
Inspissation
·
Incineration
Tyndallisa
W
·
Hot
all
over
Autoclave
-
>
Flaming
temp
:
So
o
-
400
%
C
2
.
g
.
-
Inoculation
loops
Inoculation
wires
-
>
Incineration
temp
:
-1000
-
1200c
2)
:
-Yel low
bay
materials
pf3
pf4
pf5

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STERILIZATION

Physical

Chemical

Heat based

Filtration

Radiation

HEAT

I (^) I

Dry

  • (^) DEP (^) Moist - > (^) DL ·

Flaming Inspissation

· (^) Incineration

Tyndallisa

W

· Hot all over Autoclave
  • Flaming temp :

So

o

  • 400 % C

g.

  • (^) Inoculation loops Inoculation wires
  • (^) Incineration temp : (^) -1000 - 1200c

-Yellow

bay materials

  • (^) Hot air (^) over temp- - Thes controls- Physical-display of^ temp^ . & time chemical - Brownie's tube Biological - Bacillus atropheus examples

  • Glasswares sharp surgicals chemicals -

glove powder^ ,^ liquid.

paraffic

  • Iissation

temp

: (^) 00-05c -^30 min per (^) day for^ 3 days kills vegetative stage e. g

  • serum

containing

Egg (^) containing culture (^) medias

FILTERATION

mechanism >^ memb separates larger^ a small particles

control -^ Serratia^ marcessors

e. g-

labile materials

Liquid & (^) Gas types of^ filters^ used Depth

Membrane

Candle (^) f I

22MTT Asbestos (^) f

sintered

glass f^ HEPA Of ↓ food &^ chemical ↓ industry (^) Serum , Vaccines, Surgical &^ N- masks

RADIATIONS temp-

X

control -^ Baccilus

pumilis

mech of A - Nuclia acid damage example

-> Heat-labile - > solids

type Forising rad" Non-ionising radh

high penetrar^ low penetr x-rays UV-rays X-rays

IR -^ -

h ↓ disposables surface^ disinfection & of

OT's

sutures

Aka Cold

sterilisation