Docsity
Log inSign up
Docsity
Prepare for your exams
Prepare for your exams

Study with the several resources on Docsity

Earn points to download
Earn points to download

Earn points by helping other students or get them with a premium plan

Guidelines and tips
Guidelines and tips
Sell on Docsity
Sell on Docsity
Log inSign up

Prepare for your exams

Study with the several resources on Docsity

Find documents

Prepare for your exams with the study notes shared by other students like you on Docsity

Search Store documents

The best documents sold by students who completed their studies

Search through all study resources
Docsity AINEW

Summarize your documents, ask them questions, convert them into quizzes and concept maps

Explore questions

Clear up your doubts by reading the answers to questions asked by your fellow students

Earn points to download

Earn points by helping other students or get them with a premium plan

Share documents
download point icon20 Points

For each uploaded document

Answer questions
download point icon5 Points

For each given answer (max 1 per day)

All the ways to get free points
Get points immediately

Choose a premium plan with all the points you need

Study Opportunities

Choose your next study program

Get in touch with the best universities in the world. Search through thousands of universities and official partners

Community

Ask the community

Ask the community for help and clear up your study doubts

University Rankings

Discover the best universities in your country according to Docsity users

Free resources

Our save-the-student-ebooks!

Download our free guides on studying techniques, anxiety management strategies, and thesis advice from Docsity tutors

From our blog

Exams and Study
Go to the blog

Microbial Survey Study Guide, Summaries of Microbiology

Georgia Southern University (GS)Microbiology

A study guide for the first lab practical exam on microbial survey. It includes information on identifying microorganisms, characteristics of eukaryotes and prokaryotes, the normal microbiome of humans, media used in the lab for culturing bacteria, and biosafety levels. The guide also covers laboratory rules, regulations, and proper procedures.

Typology: Summaries

2022/2023

Available from 10/11/2023

tytanisha-taylor
tytanisha-taylor 🇺🇸

1 document

1 / 7

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
LAB PRACTICAL EXAM #1 Study Guide
MICROBIAL SURVEY
Be able to identify required prepared slides = the list written on the board and
Handout-Lab2-3-Survey Microorganisms.
These included microscopic: algae, diatoms, desmids, cyanobacteria, bacteria,
animals, fungi and protozoa. etc....
●Know the characteristics of Eukaryotes and Prokaryotes. Know all the characteristics
of chart for Protozoa, Algae, and Bacteria
○Prokaryotes
■Small and simple
■0.1 to 5.0 μm in size
■Unicellular
■Nucleus is absent
■Single haploid chromosome
■Lack membrane bound organelles
■Reproduce both sexually and asexually
■Cell division by binary fission
■Examples: bacteria and archaea cells
○Eukaryotes
■Large and complex
■10 to 100 μm in size
■Unicelluar or multicellular
■Nucleus is present
■Linear DNA
■Paried diplod chromosomes
■Has membrane bound organelles
■Mostly reproduce sexually
■Cell division mitosis
■Examples: plant/animal cells, including humans
●Know what percentage of organisms that are pathogens OR helpful, beneficial, or not
harmful.
○99%
●Know all the structures microorganisms use for motility. What’s the normal
microbiome of human?
●Fungi (molds and yeasts) grow on Sabouraud’s Dextrose Agar (SDA). Why do fungi
grow better on SDA than TSA or NA (nutrient agar)?
pf3
pf4
pf5

Partial preview of the text

Download Microbial Survey Study Guide and more Summaries Microbiology in PDF only on Docsity!

LAB PRACTICAL EXAM #1 Study Guide

MICROBIAL SURVEY Be able to identify required prepared slides = the list written on the board and Handout-Lab2-3-Survey Microorganisms. These included microscopic: algae, diatoms, desmids, cyanobacteria, bacteria, animals, fungi and protozoa. etc.... ● Know the characteristics of Eukaryotes and Prokaryotes. Know all the characteristics of chart for Protozoa, Algae, and Bacteria ○ Prokaryotes ■ Small and simple ■ 0.1 to 5.0 μm in size ■ Unicellular ■ Nucleus is absent ■ Single haploid chromosome ■ Lack membrane bound organelles ■ Reproduce both sexually and asexually ■ Cell division by binary fission ■ Examples: bacteria and archaea cells ○ Eukaryotes ■ Large and complex ■ 10 to 100 μm in size ■ Unicelluar or multicellular ■ Nucleus is present ■ Linear DNA ■ Paried diplod chromosomes ■ Has membrane bound organelles ■ Mostly reproduce sexually ■ Cell division mitosis ■ Examples: plant/animal cells, including humans ● Know what percentage of organisms that are pathogens OR helpful, beneficial, or not harmful. ○ 99% ● Know all the structures microorganisms use for motility. What’s the normal microbiome of human? ● Fungi (molds and yeasts) grow on Sabouraud’s Dextrose Agar (SDA). Why do fungi grow better on SDA than TSA or NA (nutrient agar)?

★ SDA is selective for fungus because of its low pH and its ability to incubate at room temperature. TSA is just general and anything can grow on it. DEFINE characteristics of Fungi , especially the molds and yeasts seen in the lab. ★ Fungi are eukaryotic, heterotrophic, lack tissue differentiation, have cell walls, propagate by spores. Fungi: molds - Be able to identify required prepared slides of fungi (Prepared slides – what are the structures – which also help us identify the species) Penicillium, Aspergillus, Rhizopus Penicillium

○ Clothespin - hold slide ○ Test tube ○ Test tube rack ○ Petri dish ■ TSA plate ■ TSB plate ■ SDA plate ○ Slides and slide covers ○ Pipette ● Wet mount: how to make one correctly with no air bubbles. (be able to demonstrate and write a step by step description.) ○ Get a clean slide ○ Get samples from bottom of bottle using pipette. ○ Put one drop on the slide ○ Put cover slip on at an angle to avoid bubbles. ● Be able to distinguish macroscopically (macro-inspection) the following colony types in a Petri dish: mold, yeast, bacteria; counts; growth or no growth ○ Source: Cellphone ○ Source: Bottom of shoe

○ Source: Cough (from from lab partner and Me) ● Know the different types of media used in the lab for culturing bacteria and what are their properties: i.e. tryptic soy agar (TSA) or nutrient agar (NA – not used so far), both general purpose media. SDA a special purpose medium. Blood agar and tryptic soy broth were also media that was used. Be able to read the media and source from bottom of plates ○ Both- General, anything grows on it. ● Bacteria: concept of clones and colonies (e.g. from your air or ubiquity plate – What does each visible colony come from?). That is referring to the theoretical concept that

  • one microscopic bacterial cell over time develops into a macroscopic colony. ○ A colony is a whole collection of cells that arise from a single bacterial cell and is visible to the naked eye on solid media. ● Demonstration area – various items and scopes at the side table especially those that are part of the microbial survey. Be able to recognize and describe ○ Giardia lamblia ; pathogenic protozoa (has monkey face) ■ ○ Trichomonas vaginalis ; pathogenic protozoa (has flagella)

● BSL Safety Agreement. Be able to define the different Biosafety Levels and abbreviations (BSL) ○ BSL-1 labs are used to study infectious agents or toxins not known to consistently cause disease in healthy adults. ■ They follow basic safety procedures, called Standard Microbiological Practices and require no special equipment or design features. ■ Standard engineering controls in BSL-1 laboratories include easily cleaned surfaces that are able to withstand the basic chemicals used in the laboratory. ○ BSL-2 laboratories are used to study moderate-risk infectious agents or toxins that pose a risk if accidentally inhaled, swallowed, or exposed to the skin. ■ Design requirements for BSL-2 laboratories include hand washing sinks, eye washing stations in case of accidents, and doors that close automatically and lock. ■ BSL-2 labs must also have access to equipment that can decontaminate laboratory waste, including an incinerator, an autoclave, and/or another method, depending on the biological risk assessment. ○ BSL-3 laboratories are used to study infectious agents or toxins that may be transmitted through the air and cause potentially lethal infection through inhalation exposure. ■ Researchers perform all experiments in biosafety cabinets that use carefully controlled air flow or sealed enclosures to prevent infection. ■ BSL-3 laboratories are designed to be easily decontaminated. These laboratories must use controlled, or “directional,” air flow to ensure that air flows from non-laboratory areas (such as the hallway) into laboratory areas as an additional safety measure. ○ BSL-4 laboratories are used to study infectious agents or toxins that pose a high risk of aerosol-transmitted laboratory infections and life-threatening disease for which no vaccine or therapy is available. ○ The laboratories incorporate all BSL 3 features and occupy safe, isolated zones within a larger building or may be housed in a separate, dedicated building. Access to BSL-4 laboratories is carefully controlled and requires significant training.