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Anaerobic medium contains a redux indicator, resazurin, which is _______ in absence of oxygen and _____ in the presence of oxygen - โ โ colorless, pink Anaerobic indicator is colorless when oxygen is [Present/Absent] - โ โ Absent
Anaerobic indicator is pink when oxygen is [Present/Absent] - โ โ Present
PRAS stands for - โ โ Pre-reduced anaerobically sterilized medium Anaerobic medium: Two types of reducing agents - โ โ Thioglycolic acid, L-Cysteine hydrochloride Gram stain procedure - โ โ Heat fix, Crystal Violet 1m, Iodine 1m, Alcohol 20s, Safranin 1m Crystal violet stains gram__ cells ___ [+/-, color] - โ โ +, purple Gram stain: Iodine is a - โ โ Mordant
Gram Stain: Alcohol provides - โ โ Decolorization
Gram Stain: Safranin is a - โ โ Counterstain
Gram Stain: Primary stain - โ โ Crystal Violet
Gram Stain: Decolorizer - โ โ Alcohol
Gram Stain: Counterstain - โ โ Safranin
Gram Stain: Mordant - โ โ Iodine
Safranin stains gram___ cells ____ [+/-, color] - โ โ -, pink
Definition: Mordant - โ โ Dye fixative, a substance used to set dyes on tissue (or fabric) Gram Stain: Dyes stain the ___ in the cell ___ - โ โ peptidoglycan, wall Acid-Fast staining procedure is a _____ stain that is used for the identification of ____ bacteria [types] - โ โ Differential, acid- fast Acid-Fast staining is used for rapid diagnosis of ____ (______) - โ โ Tuberculosis (Mycobacterium Tuberculosis Acid-Fast staining: Waxy substance that gives acid fast bacteria higher affinity for the initial stain (carbol fuchsin) and resistance to decolorization (acid alcohol) - โ โ Mycolic acid
Acid fast bacteria stain - โ โ pink
Non acid fast bacteria stain - โ โ blue
Ziel-Neelson is a type of - โ โ Acid-fast stain
Acid-Fast stain procedure - โ โ Carbolfuschin 30s, Heat fix, Acid alcohol 15-20s, Methylene blue 30s, rinse Acid-Fast stain: Primary stain - โ โ Carbolfuschin
Acid-Fast stain: Mordant - โ โ Heat
Growth Medium: Liquid medium growth appears ____ the tube in the form of ____, _____, or _____ appearance - โ โ Throughout, dispersed, cloud, particle General purpose medium grows a _____ _____of microbes - โ โ wide variety General purpose medium is considered to be _____ and contain a variety of nutrients to support a wide range of microbes (e.g.. MH) - โ โ non-synthetic Enriched Medium: Media is prepared with ____ _____ ____ (e.g.. BA) - โ โ additional growth factors Enriched medium is used to _____ and ____ organisms that are unable to grow on ____ media and display _______ reactions - โ โ recover, investigate, basic, hemolysis Enriched medium is prepared with ____% sheep blood added to ____ base - โ โ 5, TSA Differential medium as it is also used to analyze bacteria that produce _____ - โ โ hemolysin Beta hemolysis is ____ hemolysis and has a ______ appearance of agar around the colonies - โ โ complete, clear Alpha hemolysis is ____ hemolysis and has a _____ appearance of agar around the colonies - โ โ partial, greenish (discoloration) Gamma hemolysis is ____ hemolysis and has a ______ appearance of agar around the colonies - โ โ absence of,
brown (normal reaction of the blood to the growth conditions of 37ยฐ C in the presence of carbon dioxide) Chocolate agar contains - โ โ lysed blood Lysed blood of chocolate agar _____ ____ _____ thereby producing more nutrients for bacterial growth - โ โ releases cellular components Chocolate agar is made by - โ โ Heating 10% sheeps blood to 85c Differential medium is also considered to be a ___ media - โ โ selective MacConkey agar: The _____ _____ and _____ ____ inhibit the growth of Gram-positive organisms which allows for the selection and isolation of gram-negative bacteria - โ โ crystal violet and bile salts MacConkey agar: Designed to selectively isolate Gram-___ and enteric (normally found in the intestinal tract) bacilli and differentiate them based on ____ fermentation. - โ โ negative, lactose Thayer-Martin Agar is chocolate agar with addition of _____ - โ โ antibiotics
Thayer-Martin Agar contains what three antibiotics - โ โ Nystatin, Vancomycin, Colistin Thayer-Martin Agar is used to isolate ____ bacteria - โ โ Neisseria (N. gonorrheae, N. meningitdis)
Anaerobic medium contains _____ (e.g. ______) agents which removes free oxygen from the media - โ โ reducing, thioglycollic acid Anaerobic medium contains a redux indicator ______ - โ โ Resazurin Eukaryotic fungi size ___ um - โ โ 2-
Unicellular fungi include - โ โ yeast
Multicellular fungi include - โ โ Molds, fungi, Candida
Eukaryotic protozoa ranges in size between ___ um - โ โ 5- 200 Protozoa are motile by means of - โ โ pseudopods, cilia, or flagella Microorganisms from largest to smallest - โ โ Protozoa, fungi, bacteria, viruses A form of providing all necessary nutrients to the microbe in an artificial environment is - โ โ Growth medium Growth Medium: Prepared by using ___ ____ or using _____ _____ - โ โ individual constituents, dehydrated powders
Growth Medium: Classified according to three properties - โ โ Physical, Chemical, Functional Growth Medium: Physical classifications - โ โ Liquid, semi- solid, solid
Growth Medium: Chemical classifications - โ โ Synthetic, non-synthetic Growth Medium: Functional classifications - โ โ General purpose, enriched, selective, differential, anaerobic, enumeration, assay, specimen transport Growth Medium: Solid medium gives a firm surface on which - โ โ bacteria can grow
Growth Medium: Cells that grow on solid media are - โ โ Colonies Growth Medium: A colony is produced of a ___ and is made of _____ - โ โ single cell, one type of species
