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Microbiology Study Guide: Chapter 1-4 - Prof. Todd M. Linscott, Study notes of Microbiology

This study guide covers key topics in microbiology, including historical figures, scientific method, microscopes, tools of the laboratory, and eukaryotic and prokaryotic cells. Learn about the works of antonie van leeuwenhoek, louis pasteur, lister, and koch, and understand the principles of light microscopy, cell components, and bacterial spores.

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2010/2011

Uploaded on 02/25/2011

steph3624
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Microbiology Bio 261
Study Guide
Chapter 1 Microbiology Background
Historical Material
Know the works or accomplishments of the following individuals.
Antonie van Leeuweenhoek
Louis Pasture
Lister
Koch (know the Koch’s postulates)
Spontaneous Generation
Who studied this problem?
What did they believe caused this?
Know the experiments they used to disprove spontaneous Generation
What is the scientific method?
a. How does it work
b. Know the different parts of this methods
c. What are the keys to good science
What are different areas of study under Microbiology?
d. Can you list and explain 2-3
Microbiology techniques (Chapter 3)
Microscopes
Review the principles of light microscopy
Objective lens
Ocular lens
Real and virtual image
Total power of magnification
Resolution and resolving (be able to define the components of the Abbe
equation)
Basic types of Microscopes
Bright field
Dark field
Phase-contrast
Florescence
Electron microscopy
How do we prepare a specimen?
How do different stains work?
Why would you chose one over another?
Why are they necessary at all?
Tools of the Laboratory (Chapter 4)
Eukaryotic & Procaryotic Profiles
Know the components of a prokaryotic Cell you will see it again HINT
Cell morphology (know the different shapes (ie, diplococci, streptococci, tetrad,
sarcinae, staphylococci, etcetera.)
Appendages
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Microbiology Bio 261

Study Guide

Chapter 1 Microbiology Background Historical Material Know the works or accomplishments of the following individuals. Antonie van Leeuweenhoek Louis Pasture Lister Koch (know the Koch’s postulates) Spontaneous Generation Who studied this problem? What did they believe caused this? Know the experiments they used to disprove spontaneous Generation What is the scientific method? a. How does it work b. Know the different parts of this methods c. What are the keys to good science What are different areas of study under Microbiology? d. Can you list and explain 2- Microbiology techniques (Chapter 3) Microscopes Review the principles of light microscopy Objective lens Ocular lens Real and virtual image Total power of magnification Resolution and resolving (be able to define the components of the Abbe equation) Basic types of Microscopes Bright field Dark field Phase-contrast Florescence Electron microscopy How do we prepare a specimen? How do different stains work? Why would you chose one over another? Why are they necessary at all? Tools of the Laboratory (Chapter 4) Eukaryotic & Procaryotic Profiles Know the components of a prokaryotic Cell you will see it again HINT Cell morphology (know the different shapes (ie, diplococci, streptococci, tetrad, sarcinae, staphylococci, etcetera.) Appendages

Flagella, peripasmic flagella, pilli, fimbria -function and basic structure (how are these used -chemotaxis Cell envelope -Capsules, slime layers -glycocalyx Cell wall -Function, structure, components -Read gram stain -Know figure 4.13 be able to label and diagram Cell membrane/Plasmmembrane Cytoplasm Major components and their basic functions. Bacterial Spores -Sporulation -Why are these important to pathogenicty of an organism

  • How do they form? What can trigger their formation? -What are the general stages of Endospore formation? Eukaryotic Cells -Origin of Eukaryotic cells -Organelles structure and function Rough/Smooth Endoplasmic Reticulum Mitochondria Chloroplast Plasma membrane Golgi Apparatus Virus (Chapter 13) General characteristics of viruses Different components RNA, DNA, Capsid, Envelope What is a host range? What can determine this range What are the General Morphologies of virusus How do viruses replicate How can virus replication occur in a Lab What are differences for virus replication of bacteriophages or animal viruses How can viruses influence or cause cancer? What are prions?