Microbiology Bio 261
Study Guide
Chapter 1 Microbiology Background
Historical Material
Know the works or accomplishments of the following individuals.
Antonie van Leeuweenhoek
Louis Pasture
Lister
Koch (know the Koch’s postulates)
Spontaneous Generation
Who studied this problem?
What did they believe caused this?
Know the experiments they used to disprove spontaneous Generation
What is the scientific method?
a. How does it work
b. Know the different parts of this methods
c. What are the keys to good science
What are different areas of study under Microbiology?
d. Can you list and explain 2-3
Microbiology techniques (Chapter 3)
Microscopes
Review the principles of light microscopy
Objective lens
Ocular lens
Real and virtual image
Total power of magnification
Resolution and resolving (be able to define the components of the Abbe
equation)
Basic types of Microscopes
Bright field
Dark field
Phase-contrast
Florescence
Electron microscopy
How do we prepare a specimen?
How do different stains work?
Why would you chose one over another?
Why are they necessary at all?
Tools of the Laboratory (Chapter 4)
Eukaryotic & Procaryotic Profiles
Know the components of a prokaryotic Cell you will see it again HINT
Cell morphology (know the different shapes (ie, diplococci, streptococci, tetrad,
sarcinae, staphylococci, etcetera.)
Appendages
