Docsity
Docsity

Prepare for your exams
Prepare for your exams

Study with the several resources on Docsity


Earn points to download
Earn points to download

Earn points by helping other students or get them with a premium plan


Guidelines and tips
Guidelines and tips

Microbiology Lab: Observing Fungal Conjugation in Rhizopus Strains, Lab Reports of Microbiology

The procedures for observing fungal conjugation in rhizopus strains during a microbiology lab session. Students are required to inoculate sda plates with both plus and minus strains, incubate them for several days, and observe the formation of zygospores at the point of contact between the two strains. The document also provides instructions for identifying fungal asexual and sexual structures under a microscope.

Typology: Lab Reports

Pre 2010

Uploaded on 08/18/2009

koofers-user-qb9
koofers-user-qb9 🇺🇸

10 documents

1 / 2

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
BIOL-2230-MICRO-LAB-8-Module-15-16.doc 10/25/07 Page 1 of 2
BIOL 2230 Micro Lab 8 Reeder
Module 15
Objectives 1-8
Activities: 1 Each group inoculates 2 SDA plates: 1 each of Sc (25-30° C) and Ca
(37° C); each group has 1 plate of Sc and 1 plate of Ca to observe at 48
hours through next lab meeting
Note similar colonial appearance to bacteria
2 Group: each table uses 2 screw cap tubes and 1, 10ml pipette
3 Group: each table uses 3 screw cap tubes, scissors, glass stirring rod
* cleanliness, but not strict asepsis
Module 16
Objectives 2-4, 9
Activities: 4 Group: each table inoculates 2 SDA plates; 1 for Rs (+ and strain), 1 for Pn
(dot inoculation)
Rhizopus Zygospore Kit: Use the following directions for the Rs (+ and
strains) plate:
Rhizopus is an excellent organism for demonstrating conjugation
because of the large number of zygospores formed. With top lighting, the
black zygospores (sexual spores) are easy to differentiate from the gray-
black sporangia (asexual spores).
Using a sterile inoculating loop, aseptically place a small amount of
the plus strain mycelium on one side of the agar plate and the minus strain
on the opposite side of the plate. Write the name of the organism and the
date on the dish. Incubate for 3 to 7 days at room temperature. Once the
two strains touch, observe daily. The dark row of zygospores will form at
the line of contact. Describe and/or draw your results.
When looking for the zygospores with a microscope, it may be
necessary to part the mycelium with forceps or a teasing needle. The
zygospores will form on the agar surface and will be hidden under the
mycelium which has overgrown them. To see zygospores, hold the plate
upside down and look towards the light. If zygospores are present, they can
be seen in the center as black dots.
pf2

Partial preview of the text

Download Microbiology Lab: Observing Fungal Conjugation in Rhizopus Strains and more Lab Reports Microbiology in PDF only on Docsity!

BIOL-2230-MICRO-LAB-8-Module-15-16.doc 10/25/07 Page 1 of 2

BIOL 2230 Micro Lab 8 Reeder

Module 15 Objectives 1- Activities: 1 Each group inoculates 2 SDA plates: 1 each of Sc (25-30° C) and Ca (37° C); each group has 1 plate of Sc and 1 plate of Ca to observe at 48 hours through next lab meeting Note similar colonial appearance to bacteria 2 Group: each table uses 2 screw cap tubes and 1, 10ml pipette 3 Group: each table uses 3 screw cap tubes, scissors, glass stirring rod

  • cleanliness, but not strict asepsis Module 16 Objectives 2-4, 9 Activities: 4 Group: each table inoculates 2 SDA plates; 1 for Rs (+ and – strain), 1 for Pn (dot inoculation) Rhizopus Zygospore Kit: Use the following directions for the Rs (+ and – strains) plate: Rhizopus is an excellent organism for demonstrating conjugation because of the large number of zygospores formed. With top lighting, the black zygospores (sexual spores) are easy to differentiate from the gray- black sporangia (asexual spores). Using a sterile inoculating loop, aseptically place a small amount of the plus strain mycelium on one side of the agar plate and the minus strain on the opposite side of the plate. Write the name of the organism and the date on the dish. Incubate for 3 to 7 days at room temperature. Once the two strains touch, observe daily. The dark row of zygospores will form at the line of contact. Describe and/or draw your results. When looking for the zygospores with a microscope, it may be necessary to part the mycelium with forceps or a teasing needle. The zygospores will form on the agar surface and will be hidden under the mycelium which has overgrown them. To see zygospores, hold the plate upside down and look towards the light. If zygospores are present, they can be seen in the center as black dots.

BIOL-2230-MICRO-LAB-8-Module-15-16.doc 10/25/07 Page 2 of 2

Sheet provided noting fungal asexual and sexual structures to D & L from prepared slides into Mod 16 (representatives from fungi’s four divisions)