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MICROBIOLOGY MIDTERM CH 1-5 2025
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The property of endospores that led to confusion in the experiments on spontaneous generation is their? - correct answers heat resistance The Golden Age of Microbiology was the time when? - correct answers most pathogenic bacteria were identified Microorganisms play a role in? - correct answers disease, biodegradation, cheese production, nitrogen recycling, all of the above Which disease was once thought to due to stress but is now known to be caused by a bacterium? - correct answers peptic ulcers The prokaryotic members of the microbial world include? - correct answers bacteria & archaea The Archaea? - correct answers are microscopic, commonly found in extreme enviroments Prokaryotes typically do not have? - correct answers a nuclear membrane Nucleoids are associated with? - correct answers genetic information & prokaryotes Viruses - correct answers contain both protein and nucleic acid & infect all domains of life
Antony van Leeuwenhoek could not have observed? - correct answers viruses Which of the following is most likely to be used in a typical microbiology lab? - correct answers bright-field microscope When a medical technologist wants to determine if clinical specimen contains a Mycobacterium species, which should be used? - correct answers acid fast stain Penicillin - correct answers Is generally effective against gram-positive bacteria & kills only growing cells Endotoxin is associated with - correct answers gram-negative bacteria The "O157" in the name E. coli 0157:H7 refers to the type of O antigen. from this information you know that E. coli - correct answers gram- negative Eliminating which structure is always deadly to cells - correct answers cytoplasmic membrane Which of the following do bacterial cells use for attachment? - correct answers capsule & pilus Endocytosis is associated with - correct answers eukaryotic cells
In proteins, "a" helices and "b" pleated structures associated with the: - correct answers secondary struture Complementarity plays a major role in structure of: - correct answers DNA A bilayer is associated with: - correct answers complex polysaccharides Isomers are associated with: - correct answers Carbohydrates & amino acids If there are 103 cells per ml at the middle of log phase, and generation time of the cells is 30 minutes, who many cells will there be 2 hours laters? - correct answers 1.6 x 10^ Compared with their growth in the laboratory, bacteria in nature generally grow - correct answers more slowly Cells are most sensitive to penicillin during which phase of the growth curve? - correct answers Exponential Lactic acid is a primary metabolite. If a company wants to harvest this compound from a bacterial culture, the cells should be in which growth phase? - correct answers Exponential
E. coli, a facultative anaerobe, is grown for 24 hours on the same solid medium, but under two different conditions: one aerobic, the other anaerobic. The size of the colonies would be: - correct answers Larger when grown under aerobic conditions The generation time of a bacterium was measured at two different temperatures. Which results would be expected of a thermophile? - correct answers 220 minutes at 37C; 20 minutes at 65C Which of the following is false? - correct answers some eukaryotes can fix N If the pH indicator were left out of MacConkey agar, the medium would be - correct answers complex A soil sample is place in liquid and number of bacteria in the sample determined in two ways: (1) colony count & (2) direct microscopic count. How would the results compare?
- correct answers Many more bacteria would be estimated by method 2 The concentration of E. coli in a broth is between 104 & 106 cell per ml. To determine the precise number of living cells in the sample, it would be best to: - correct answers plate out an appropriate dilution of the sample on nutrient agar Unlike a disinfectant, and antiseptic - correct answers is nontoxic enough to be used on human skin The D value is defined as the time it takes to kill - correct answers 90% of bacteria in a population