Download MICROBIOLOGY MIDTERM EXAM WITH CORRECT AND VERIFIED ANSWERS 2025 - JERSEY COLLEGE and more Exams Nursing in PDF only on Docsity!
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
Organisms are commonly encountered in the community and present a moderate health hazard. - correct answers BSL- 2 Organisms have a great potential for lethal infection; typically spread via aerosol. - correct answers BSL- 3 Organisms do not typically cause disease in healthy individuals and present minimal threat to environment and lab personnel. - correct answers BSL- 1 Organisms are of local or exotic origin and are associated with respiratory transmission and serious or lethal diseases where treatment and/or vaccines may or may not be available - correct answers BSL- 4 Most introductory Microbiology courses use organisms that may be handled at
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
biosafety levels ______________ and ______________. - correct answers BSL-1 & BSL- 2 Biosafety cabinets, such as a chemical fume hood, are used to draw _____________ into the cabinet to minimize microbial contamination back into the room. - correct answers AIR Blue, red and yellow boxes on the chemical hazard label indicate _____________________, _________________________, and _______________________ levels, respectively. - correct answers HEALTH, FLAMMABILITY, REACTIVITY Regarding student conduct, list at least 3 things that one should do or not do to reduce the risk of infection. - correct answers DO NOT SMOKE, EAT, DRINK, OR BRING FOOD INTO THE LAB What type of shoes must be worn in the lab? - correct answers CLOSED-TOE SHOES What should be done, if one has long hair? - correct answers SHOULD BE TIED BACK If you are pregnant, immune compromised or ill, what should you do? - correct answers SEE INSTRUCTOR
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
Regarding hand washing, when must one wash hands when working in the Microbiology laboratory? - correct answers BEFORE & AFTER
. _____________________ is used to grow bacterial and fungal cultures. - correct answers MEDIA The term, ______________________, means without contamination or "as sterile as possible". - correct answers STERILE To be of practical use, nutrient broth and nutrient agar are formulated from sources that supply _____________________ and ________________________ in a variety of forms - correct answers CARBON & NITROGEN The term, __________________________, or _______________________ media refers to media in which the ingredients are unknown or in unknown amounts - correct answers UNDEFINED & COMPLEX The term, chemically ______________________, refers to media that are composed of ingredients with a known chemical structure and/or amount - correct answers DEFINED
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
. ________________ is an agent in the media that is used solely to make the media solid or semi-solid depending on the concentration. - correct answers AGAR When pouring plates, the lid should be used as a ______________________ to prevent or minimize the chance of introducing airborne contaminants. - correct answers SHIELD The three conditions that must be met to ensure the media is sterilized while in the autoclave are: pressure should be set at 15 pounds per square inch; temperature should be set at ________________; and a minimum time of
should be met. - correct answers 121 c & 15 MIN Microbiologists must be able to transfer living microorganisms from one medium to another, __________________________, or without contamination of the culture. - correct answers ASEPTICALLY Wire inoculating loops and needles are sterilized by passing them through an open flame on the _________________________ burner. - correct answers BUNSEN A medium that contains living microbes is called a _______________________________. - correct answers CULTURE
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
The inoculating loop or needle should be held like a _____________________________. - correct answers PENCIL The instruments usually used for transfers are either _____________________________ or ______________________________________. - correct answers INOCULATING LOOP OR INOCULATING NEEDLES If handling a BSL-2 organism, one should use techniques that minimize _____________________ production - correct answers AEROSOL Typically used for obtaining isolation of a species. - correct answers PLATES Used to grow microbes in bulk quantities or when a large number is required. - correct answers BROTHS Used to grow stock cultures that can be stored for several weeks in the refrigerator. - correct answers AGAR SLANTS A microbial culture consisting of two or more species is said to be a __________________ culture. - correct answers MIXED
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
A commonly used isolation technique is the ___________________ plate - correct answers STREAK _______________________ are cells that have been sufficiently isolated and consist of only the original cell type. The term _______________________________ is a more correct description of this - correct answers COLONIES & COLONY-FORMING UNIT (CFU) A _____________________ streak is generally used with samples of high cell density, whereas a ________________________ streak pattern may be used for samples containing lower cell densities. - correct answers QUADRANT & CONTINUOUS Microorganisms are described as being ________________________ in nature, meaning they exist everywhere. - correct answers UBIQUITOUS Decompose organic matter. - correct answers Saprophytes Cause damage to host; disease causing - correct answers Opportunistic pathogens Microorganism actually benefits the host - correct answers Mutualism
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
The shape of the colony may be __________________, irregular, or ________________________. - correct answers ROUND & PUNCTIFORM Branched like roots. - correct answers Rhizoid Lobed - correct answers Undulate Smooth, no irregularities. - correct answers Entire Wavy - correct answers Lobate Unbranched strands - correct answers Filamentous The surface of a colony may be _________________, rough, _____________________, shiny, or __________________. - correct answers SMOOTH, WRINKLED, DULL The texture of the colony may be moist, __________________ (sticky), _____________________ (buttery), or dry - correct answers MUCOID & BUTYROUS The elevation of the colony may be flat, raised, convex, ________________________ (very
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
convex), or _______________________ (raised in the center). - correct answers PULVINATE & UMBONATE The term, _____________________, means you cannot see through it. The term, _____________________, means light passes through it. - correct answers OPAQUE &TRANSLUCENT Branched. - correct answers Rhizoid Spreading - correct answers Effuse Dense and opaque with a smooth edge. - correct answers Filiform Spiny. - correct answers Echinulate Dry or crusty - correct answers Friable Clumps throughout the tube - correct answers Flocculent Cloudy throughout the tube - correct answers Uniform fine turbidity
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
population density in a liquid sample by plating a very dilute portion of that sample and counting the number of colonies it produces. - correct answers STANDARD The inoculum that is plated is a product of a _________________ dilution and thus calculations can be made to estimate the amount in the original sample. - correct answers SERIAL By convention, dilutions are expressed in ______________________ notation. Therefore a 1/10 dilution is written as ____________ and a 1/100 dilution is written as ____________. - correct answers SCIENTIFIC, 10^-1, 10^- 2 A countable plate is between _____________ and ____________ colonies. - correct answers 30 & 300
. A count lower than ____________ is considered statistically unreliable and a count greater than _____________ is too numerous to count. - correct answers 30 & 300 The light in bright-field microscopy is transmitted _____________________ a specimen. - correct answers THROUGH Because most biological specimens are transparent, the contrast between the
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
specimen and the background can be improved with the application of ______________ - correct answers STAINS The _________________ lens concentrates the light by acting like a funnel of which makes illumination of the specimen more uniform. - correct answers CONDENSER Light passes through the specimen, followed by passing through the __________________ lens producing a magnified real image, and is then magnified again by passing through the __________________ lens to produce a virtual image. - correct answers OBJECTIVE, OCULAR The term, ______________________, refers to the bending of light as it passes through the lenses. - correct answers REFRACTION Total magnification of a specimen can be calculated by multiplying the __________________ lens by the ____________________ lens. - correct answers OBJECTIVE, OCULAR Clarity of an image is called ________________________. The limit of resolution, or ________________________ power is an actual measurement of how far apart two points must be for the microscope to view them as being separate. - correct answers RESOLUTION, RESOLVING
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
When carrying the microscope, one hand should be grasping the microscope's _______________ and the other supporting the microscope beneath its ________________. - correct answers ARM, BASE
. __________________________ should be used to clean the microscope's objective lenses and condenser lens. - correct answers LENS PAPER To clean the ocular lenses, one should use a _________________________________, moistened with cleaning solution - correct answers COTTON SWAB When beginning, one should start viewing of the specimen using the _________________ objective or the _______________________ objective lens. - correct answers SCANNING, LOW POWER Adjusting the _______________________________ can produce optimum illumination, contrast and image. - correct answers IRIS Use the _____________________ adjustment knob to bring the initial image into focus. - correct answers COARSE FOCUS
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
To bring the image into sharpest focus, use the ____________________ adjustment knob. - correct answers FINE FOCUS
. The term, _______________________, refers to the fact that all should be necessary after changing objective lenses is slight adjustment. - correct answers PARFOCAL Since the cytoplasm of cells is transparent, stains must be used to provide _________________ with the background of the microscope field. - correct answers contrast Short rods. - correct answers Coccobacilli Spiral shaped - correct answers Spirilla (spirillum) Flexible spirals - correct answers Spirochetes Rod shaped - correct answers Bacilli (bacillus) Slightly curved rods - correct answers Vibrios Sphere or round shape - correct answers Cocci (coccus)
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
The _________________________ is the charged portion of the chromogen that allows it to form ionic or covalent bonds between the chromogen and the cell. - correct answers auxochrome ______________________ stains are positively charged and thus are attracted to ______________________ charges on the surface of most bacterial cells. - correct answers basic, negative Common basic stains include methylene blue, _______________________________, and ______________________. - correct answers crystal violet, safranin
. _______________________ the bacteria prior to adding stain kills the bacteria and makes them adhere to the slide - correct answers heat fixing The ______________________ stain uses a dye in which the chromogen is acidic and thus carries a ___________________ charge - correct answers negative, negative The bacterial cell repels the negative charge and thus remains _______________________ against a colored background; of which is why it is called a negative stain. - correct answers unstained The negative stain technique is used to determine shape and arrangement of
CORRECT AND VERIFIED ANSWERS 2025 -
JERSEY COLLEGE
bacteria that are too delicate to withstand ____________________________. - correct answers heat fixing _________________________ stains allow a microbiologist to detect differences between organisms or differences between parts or structures of the same organism - correct answers differential The ____________________ stain is the most commonly used differential stain in bacteriology - correct answers gram Other differential stains will stain for other important cellular attributes such as acid-fastness, a _____________________, _______________________, or flagella. - correct answers capsule, spores The first stain used in a differential staining method is called the __________________ stain, and in the case of the Gram stain, it is crystal violet. - correct answers primary Iodine used in the Gram stain will act as a ____________________, to enhance the crystal violet staining by forming a ____________________________________________________. - correct answers mordant, crystal violet-iodine complex
