Download MICROBIOLOGY - MIDTERM PRACTICE QUESTIONS WITH CORRECT AND VERIFIED ANSWERS 2025 and more Exams Nursing in PDF only on Docsity!
QUESTIONS WITH CORRECT AND VERIFIED
ANSWERS 2025
Intro quiz - correct answers Which of the following should be worn at ALL times in the lab? (Check all that apply) - correct answers Lab Coat & Closed toed shoes Which of the following would be most appropriate for disposal of cultures on agar plates? - correct answers biohazard bag or bin You may eat a snack in lab as long as there are no bacterial cultures on your bench and you have thoroughly washed your hands. - correct answers False This image illustrates what correct safety practices? (Check all that apply) - correct answers Use of gloves and eye protection, Tying back of, Wearing of the lab coat, Using a biohazard container, Using a biohazard container Infectious laboratory waste is sterilized prior to disposal by - correct answers autoclaving (steam autoclave) Which of the following items can be washed in the sink after use in the laboratory? (Check all that apply) - correct answers dissection tools, glassware that was not used with hazardous, E. coli broth culture tubes, inoculating loops, dissection trays Watches, bracelets, and rings should be removed prior to washing hands. - correct answers True
QUESTIONS WITH CORRECT AND VERIFIED
ANSWERS 2025
What biosafety levels do most introductory microbiology students work with? - correct answers BSL- 1 How long should hands be lathered while washing? - correct answers 20 seconds Gloves provide protection against (Check all that apply) - correct answers infectious mircroorganisms, contamination of cultures with skin bacteria from the hands, broken glass and sharps, exposure to chemicals and stains. Jackie went to the sink at the end of lab to wash her hands. After removing her watch and rings, she turned on the water, wet her hands, added soap and thoroughly rubbed and lathered her hands for 30 seconds, cleaning the backs of her hands, her fingernails, and between her fingers. She then turned off the water, and reached for the paper towels to dry her hands. Identify the correct statement below. - correct answers Jackie should have used paper towels to turn off the faucet. Which of the following liquids can be safety pipetted by mouth? (Check all that apply) - correct answers a glucose solution, a broth culture of Staphyloccus epidermidis Beverages are allowed in the laboratory as long as the containers are covered. - correct answers False
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ANSWERS 2025
The total magnification achieved using a 10× objective lens with a 10× eyepiece lens is 20×. - correct answers False You have successfully focused on an object with the 10x and 40x objective lenses. You have rotated the 100x objective lens into position but are unable to obtain a sharp image by rotating the fine focus adjustment knob. Which of the following is most likely to correct this problem? - correct answers Add a drop of immersion oil before rotating the 100x lens into position. Using oil immersion is useful because - correct answers oil immersion increases the resolving power of the specimen. _______ fungi are those species that occur as either molds or yeast, depending on environmental conditions such as temperature and nutrient availability. - correct answers Dimorphic Which of the following does not describe cyanobacteria? - correct answers Have bacteriochlorophyll The three major means of locomotion in free-living protozoa are - correct answers pseudopodia, cilia, flagella, fimbriae Please choose the answer that best fills in the blanks of this sentence in the correct order. Algae and protozoa are in Domain ______ and cyanobacteria are in Domain _____.
- correct answers Eukarya; Bacteria
QUESTIONS WITH CORRECT AND VERIFIED
ANSWERS 2025
Eukaryotes such as animal and plants cells differ from prokaryotes in that prokaryotes
- correct answers lack a true nucleus. The cell is the basic structural/functional unit common to all living organisms. - correct answers True A defining characteristic that distinguishes prokaryotic and eukaryotic organisms is the lack of a cell membrane in prokaryotes. - correct answers False What type of organism is Elodea? - correct answers Plant Identify the statements that are true of cells - correct answers -- Organisms are composed of one or more cells, and the life processes of metabolism and heredity occur in these cells. -- Cells routinely form from non-living molecules. -- Cells are the smallest living thing, the basic units of organization of all organisms. Fermentation Quiz - correct answers
QUESTIONS WITH CORRECT AND VERIFIED
ANSWERS 2025
How does pH negatively affect the metabolism of microorganisms? - correct answers pH changes can denature enzymes Both human pathogens and normal microbiota are typically classified as: - correct answers mesophiles You are studying a bacterium found in the normal intestinal flora of humans and most other mammals, and are doing an experiment to determine the optimum growth rate of this bacteria. You have inoculated five nutrient broths, and are incubating one broth at each of the following temperatures: 5oC, 25oC, 38oC, 42oC, and 55oC. At which temperature would you predict to see the optimum growth rate? - correct answers 38C Select all of the following pathways that produce ATP during cellular respiration. - correct answers glycolysis, Calvin cycle, Krebs cycle, preparatory reaction A process common to all living organisms, whether aerobic or anaerobic, is - correct answers glycolysis. Select all of the following that are products of alcoholic fermentation. - correct answers water, oxygen, ethanol, carbon dioxide Which of the following processes will occur in the presence or absence of oxygen? - correct answers glycolysis
QUESTIONS WITH CORRECT AND VERIFIED
ANSWERS 2025
What is produced when beer is made? - correct answers CO2 and ethanol All of the following are the end products of glycolysis except - correct answers NAD+ Culturing Quiz - correct answers What incubation temperature is preferred for the growth of most medically important bacteria? (Check all that apply) - correct answers 37C , 45C Process of adding a microbe to a growth material - correct answers inoculation The material which provides the nutrients for growth - correct answers medium To cultivate (verb) or observable growth (noun) - correct answers culture Which types of culture media are solid? (Check all that apply) - correct answers agar plates, broth, slants Where should the label be placed on Petri dishes? - correct answers on the bottom
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ANSWERS 2025
Organism that uses inorganic carbon dioxide as its carbon source - correct answers Autotroph Organism that must obtain its carbon in an organic form - correct answers Heterotroph Organism that gains energy from chemical compounds - correct answers Chemotroph Organism that gains energy through photosynthesis - correct answers phototroph MacConkey agar isolates gram-negative enteric bacteria by inhibiting growth of gram- positive bacteria. - correct answers Selective Staphylococcus aureus can be identified on mannitol salt agar (MSA) because it ferments the mannitol, changing the color of the agar from pink to yellow. - correct answers Differential Also known as synthetic media, this type of medium has a defined molecular content and varies very little from one source to another. - correct answers Chemically defined
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ANSWERS 2025
Also known as non-synthetic media, these media cannot be chemically defined because they are partly produced from organic extracts of tissues, cells, or other biological materials. - correct answers Complex Media The terms crateriform, umbonate, convex, flat, and raised are all used to describe the _______ of a colony. - correct answers elevation Which of the following terms are used to describe the appearance of the edge or margin of a colony growing on an agar surface? - correct answers Smooth, Wavy, Irregular. Indicate which of the following observations may be encompassed by the term "cultural characteristics." - correct answers -- Gram stain morphology -- Appearance of growth on nutrient agar plates -- Appearance of growth and presence of hemolysis on blood agar -- Acid-fast morphology -- Appearance of growth in fluid thioglycollate medium -- Appearance of cells stained with an endospore stain Dilution Quiz - correct answers When preparing pure cultures, dilution is necessary for - correct answers reducing the number of inoculated organisms so that isolated colonies can develop.
QUESTIONS WITH CORRECT AND VERIFIED
ANSWERS 2025
When determining the cell density of a sample by the standard plate count method, the final density of cells is reported as - correct answers colony forming units (CFU)/mL If you wanted to prepare a 1:10,000 dilution of your sample and cannot measure out quantities of less than 1 mL, and have dilution blanks that can hold only 10 mL, how many dilution blanks will you need? - correct answers 4 Microbial Control Quiz - correct answers The most versatile method of sterilizing heat-sensitive liquids is - correct answers filtration Disinfection of beverages, such as apple juice, milk and wine, is optimally achieved by - correct answers pasteurization What would Enterococcus' susceptibility to Ampicillin be if you measured a 16 mm diameter around your disk? Use the standardized tables in the lab manual to help you.
- correct answers Resistant A compound such as cephalothin that is made by a species of mold and is able to kill or inhibit a number of different bacteria is correctly referred to as - correct answers an antibiotic
QUESTIONS WITH CORRECT AND VERIFIED
ANSWERS 2025
What is the zone of inhibition for Vancomycin (VA) in this Kirby-Bauer test? (mark is at the 2nd line to the right of the line for number 2 on a metric ruler) - correct answers 18mm How is an antiseptic different from a disinfectant? - correct answers An antiseptic is used to remove or kill microorganisms on tissue, whereas a disinfectant is used to remove or kill microorganisms on inanimate objects. An agent that stops the growth of bacteria but does not kill them is described as - correct answers bacteriostatic. Which parts of a cell are most affected by temperature? - correct answers Ribosomes, Membrane fluidity, and enzymes Endospores are less susceptible to UV damage because ________. (Check all that apply.) - correct answers -- UV light generates a product in endospores that functions in the enzymatic repair of damaged DNA -- the DNA of endospores is protected by small proteins that protect it from photochemical damage Stains Quiz - correct answers The Gram stain is an example of a _______ staining procedure, which takes advantage of the fact that cells or parts of cells react differently and can be distinguished by the use of two different dyes. - correct answers differential
QUESTIONS WITH CORRECT AND VERIFIED
ANSWERS 2025
Please choose the answer that best completes the blanks of this sentence in the correct order. After acid-fast staining, Mycobacterium tuberculosis would be _______ in color, whereas Proteus vulgaris would be ________ in color. - correct answers red; blue What makes Mycobacterium particularly resistant to staining? - correct answers The presence of mycolic acid in their cell walls Please select all of the statements that are true about endospores. - correct answers -- Bacterial endospores are highly resistant to heat, drying and radiation. -- Endospores can exist in the environment for indefinite periods of time. -- Endospores resist boiling and therefore steam must be used to destroy endospores present in food. After capsular staining, the cells are clear and the capsule is a dark purple ring. - correct answers False
