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MLT Practice Exam Questions and Answers 2025 -
Agents brucellosis epsilon toxin food contaminants glandes melioidosis psittacosis Q fever and ricin toxin are classified as ✔✔category B agents ( second highest priority and include pathogens that are moderately easy to disseminate)
What regulation that become law in 1992, requires that laboratories develop implement and comply with a plan that ensures the protective safety of laboratory staff to potential infectious blood-borne pathogens and manage and handle medical waste in a safe and effective manner.
✔✔occupational exposure to bloodborne pathogens
What category of bioterrorism agents has the following characteristics?
Pathogens that are rarely seen in the United States these agents have the highest priority; organisms in this category pose a risk to national security because they can be easily disseminated or transmitted from person to person result in high mortality rates and have the
potential for major public health impact ✔✔category A ( A has the highest priority)
All employees who work with hazardous chemicals will be required to be trained about the new
labels and the elements of the Safety Data Sheet ( SDS ) by December 1 of ✔✔ 2013
All chemical manufacturers and distributors must comply with all of the new provisions in the
revised standards by June 1 ✔✔ 2015
in 2012 the US Department of Labor of Occupational Safety and Health Administration finalized their new Hazard Communication Standard.
What do the letters GHS stand for ✔✔Globally Harmonized System
Material Safety Data Sheets (MSDSs) are now called ✔✔Safety Data Sheets
in the clinical chemistry laboratory where the vest majority of the total laboratory analyses are performed most measurements are concerned with the concentrations of substances in the solutions and these substance being measured are know as ✔✔solute
how to convert Celsius to Kelvin ✔✔Celsius plus 273
A microscope should never be left with the ✔✔oil objective in place
if a 10 x eyepiece is used with a 45 x objective the total magnification is ✔✔450 ( 10 x 45 = 450)
what is the order of draw of multiple evacuated tubes ✔✔blood culture (aerobic) blood culture (anaerobic) blue red green stopper lavender and gray
five specific situation may result in a difficult venipuncture or may be a sources of preanalytical error. they are ✔✔IV line, edema, scarring or burn pt, dialysis pt and mastectomy pt
the role of the phlebotomist has never been more important to the pt and lab. how many errors are caused by preanalytical errors ✔✔two thirds
what are the two general sources of blood for clinical lab test ✔✔peripheral ( or capillary ) blood and venous blood
In POCT testing glucose is commonly measured and used what type of sensor ✔✔Biosensor
which of the following may be said to be true of fluorescent spectroscopy ✔✔high sensitivity and high specificity
The immediate precursor of bilirubin is ✔✔Biliverdin
The reduction of oxaloacetate to malate by malate dehydrogenase is used in the measurement of
which enzyme ✔✔AST
You have a pt's sample that has a slightly elevated ALT, LD and bilirubin with a very elevated GGT and an elevated alkaline phosphatase. What might be the pt's diagnosis? ✔✔intrahepatic cholestasis
the type of diabetes results from lack of insulin caused by cell-mediated autoimmune destruction
of insulin-secreting pancreatic beta (b) cells ✔✔Type 1
in fat catabolism (the phase of metabolism in which fats are broken down for energy) what is produced first? ✔✔Acetoacetic acid
Amyloid B 42 and tau protein test are used to differentiate a diagnosis of ✔✔Alzheimer's disease from other forms of dementia
Aspartate aminotransferase (AST) is an enzyme that belongs to which class ✔✔Transverses
Which cardiac biomarker is freely filtered by the kidney which allows levels to return to normal
18 to 30 hours after the AMI? ✔✔Myoglobin
An increased ALP level in an adult would most likely be caused by ✔✔Liver disease
Which enzyme is widely distributed in the body is elevated on a variety of disorders and
catalyzes the interconversion of lactic and pyruvic acids ✔✔Lactate dehydrogenase
What hormone is hydrophobic and insoluble in water ✔✔Steroid hormones
What is secreted by the alpha cells of the pancreas is the major hormone that opposes the action of insulin increasing blood glucose by stimulating the breakdown of glycogen (glycogenolysis) by the liver ✔✔Glocagon
This is one of the most useful procedures for distinguishing immune from nonimmune mechanisms that can underlie hemolytic anemias. what test is used to detect RBCs that have been coated with antibodies? ✔✔Direct antiglobulin test (DAT)
THE life span of a neutrophil is spent in three compartments. which compartment is the site of differentiation proliferation a maturation? ✔✔Bone marrow
A differential shows extreme leukocytosis normal RBC morphology with possible NRBCs and exhibits a shift to the left with all stages of granulocyte maturation. which disorder is this ✔✔Chronic myelogenic leukemia
The characteristics of this megakaryocytic cell is 14-30um in diameter and has primarily basophilic cytoplasm indented or bilobed nucleus and few azurphilic cytoplasmic granules. ✔✔Promegakaryocyte
Where is the primary site of megakaryocyte development and platelet production ✔✔Bone marrow
An erythrocyte still possessing RNA is referred to as a reticulocyte. What supravital stains bind neutralize and cross-link ribonucleic acid (RNA) ✔✔New methylene blue method
what has been selected as the method of choice by the Clinical Laboratory Standards Institute
(CLSI) for measuring erythrocyte sedimentation rate (ESR) ✔✔Westergren method
which principal automated hematology analyzer allows the simultaneous analysis of multiple
characteristics through a laser light beam ✔✔flow cytometry
A quality assessment program is divided into three components. Which component deals with review of patients results maintenance of patient records and maintenance of all documentation?
✔✔post examination
quality control is a mandate to ensure accurate testing and reporting of results. Which type of quality control process instituted monitors the testing process by comparing the specific
laboratory to peer laboratories. ✔✔proficiency testing
what is released from the platelets as they adhere to the margins of the injury in the wall of the blood vessel? It promotes local direct biochemically stimulated narrowing of the torn blood
vessel and of locally intact blood vessels in the same vicinity as the injury. ✔✔serotonin
The activation of what factor occurs by means of either the extrinsic or intrinsic pathway or a
combination of both pathways to form the common pathway. ✔✔X and Xa
Thrombin is generated from a precursor of this substance and is involved in the common pathway of both the extrinsic and the intrinsic clotting pathways. What is synthesized by the liver
through the action of vitamin K? ✔✔prothrombin
What is a test system to assess platelet related primary hemostasis with greater accuracy and reliability than bleeding time? ✔✔closure time (CT)
Prothrombin time measures which pathway ✔✔Extrinsic pathway
Current test for platelets include ✔✔Platelet count platelet aggregation assays platelet adhesion and clot retraction.
The APTT measures factors XII XI IX VIII V II and ✔✔fibrinogen
What is a test system to assess platelet related primary hemostasis with greater accuracy and
reliability than bleeding time ✔✔Closure time (CT)
What provides an alternative to immunofluorescent assays and uses nonisotopic label which offers the advantage of safety and demonstrates specificity sensitivity and rapidity? This method
is popular for waived over the counter testing. ✔✔Enzyme immunoassay (EIA)
What is a technique in which proteins are separated electrophortically transferred to membranes and identified through the use of labeled antibodies specific for the protein of interest?
✔✔Western blot
What are defined as antibodies that are stimulated by one antigen and react with an entirely
unrelated surface antigen present on cells from different mammalian species. ✔✔Heterophil antibodies
Which theory with regards to Rh believes that each parent gives one Rh gene but that alleles at one gene locus are responsible for expression of their system antigens on the red cell?
✔✔Weiner
The physical expression of inherited traits is determined by reacting red cells with know antisera
and observing for the presence or absence of hemagglutination is ✔✔phenoytpe
Anti- A and Anti B ✔✔AB
If you mix an unknown antigen ( pts red cells) with a known antibody (Anti-A) and get visible agglutination which unknown antigen is detected ✔✔A
Anti- A + Anti B 0 ✔✔A
What is the source of antibody for performing the forward group ABO/D typing ✔✔commercial antisera (Anti A anti B Anti D)
One method to avoid reactivity with cold antibody is ✔✔perform a pre-warm technique
During a differential direct antiglobulin test procedure the pt was positive with monospecific
anti-C3d. What is the interpretation ✔✔pt red cells are sensitized with IgG only
MacConkey is negative for growth. The colonies are described as large with small diffuse zones of B-hemolysis that were observable when the colony was removed from the agar. The gram stain reveals a regular short gram-positive coccobacilli with "tumbling" motility. The isolate is catalase positive and esculin hydrolysis positive. What is the presumptive identification?
✔✔Listeria monocytogenes
What is the most common cause of fungemia that is increased in immunocompromised pts
✔✔Candida albicans
A gram negative rod>100,000 col/cc is recovered from a midstream urine. The isolate is swarming over the blood plate produces hydrogen sulfide on TSIA and hydrolyzes urea indole
negative and ornithine positive. What is the likely organism identification ✔✔Proteus mirabilis
Which organism needs the X and V factors required for fastidious organisms? ✔✔Haemophilus spp
What test contains an enzyme that breaks down hydrogen peroxide into oxygen and water. ✔✔Catalase test
When deciding which antimicrobial agents to test and report on, an isolate which of the following statement is not true ✔✔the resistance patterns of the institution do not need to be regarded in the selection of antibiotics to report
Which of the following organisms is commonly tested for B-lactamase production by the cefinase method? ✔✔Streptococcus pneumoniae
Sorbitol-MacConkey agar (SMAC) can be used to detect which stool pathogen? ✔✔Escherichia coli 0157:H
What method is used for quantitating the growth of microorganisms in the urine and is the classic
culture method typically used in many clinical laboratories ✔✔Streak plate method
Disk diffusion or the Kirby-Bauer method of susceptibility testing is performed by ✔✔use of antibiotic impregnated filter paper disks placed on an agar plate that has been seeded with a lawn of bacteria
The phylum Cestodes are ✔✔tapeworms
To detect helmith eggs and motile trophozoite stages of the protozoa use ✔✔direct wet mount
What is the most commonly used stain for parasitic examination of stool samples ✔✔Wheatley trichrome
Dematiaceous fungi produce ✔✔dark or pigmented hyphae
In the process of forming urine simple filtration takes place in which part of the kidneys
✔✔Glomerulus
What is a high molecular weight glycoprotein (mucoprotein) that is normally secreted by renal tubular epithelial cells ✔✔Tamm-Horsfall protein
All the following about a 24 hour collection beginning at 7 am are true except ✔✔discard the last urine collected in the 24 hour urine collection period
Identification of oval fat bodies is confirmed by staining with ✔✔Sudan III
Clinitest is used to confirm ✔✔glucose
The urine collection used to evaluate orthostatic proteinuria is ✔✔A first and second morning specimen
which of the following crystals should be accompanied by a positive test for urine bilirubin
✔✔tyrosine
a pear shaped fiagellate with an undulating membrane commonly found in urine sediment is
✔✔Trichomonas
in using the reagent strip test for protein it is more sensitive to ✔✔albumin
which of the following test performed on amniotic fluid is used to screen for Fetal Lung Maturity
✔✔L/S ratio
Cellular cast should first be examined under ✔✔low power
Waxy Casts are almost always pathologic because they ✔✔indicate renal disease
A specimen just collected in a spinal tap is taken immediately to the lab and examined. The specimen is centrifuged in a microhematocrit tube. The supernatant is xanthochromic most likely
indicating ✔✔the pts had a previous brain bleed
All but can be found in increased levels with urines having a maple syrup odor
✔✔tyrosine
Al of the following are normally found in urine except ✔✔glucose
All the following crystals are found in alkaline urine except ✔✔amorphous urates
The reagent strip test for pH uses a double indicator system which uses what two chemicals
✔✔bromthymol blue and methyl red
A freshly voided urine was yellow and clear. It was placed in the refrigerator until it could be brought to the lab. When it arrived in the lab it had a white precipitate. Which of the following is
the most likely explanation for this urines appearance upon arrival in the lab. ✔✔amorphous phosphates precipitated
Acetest tablet test is used to confirm ✔✔acetone and acetoacetic acid
