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An investigation into observing cellular respiration in yeast, a one-celled fungus that undergoes this process to reproduce and survive. Background information on cellular respiration, the role of the mitochondrion and cytoplasm, and the differences between aerobic and anaerobic respiration. The experiment involves observing the inflation of a balloon as a result of cellular respiration in yeast. The data collected is analyzed through creating a line graph, and the conclusions drawn discuss the evidence of cellular respiration, the energy source for the yeast, the gas produced, and the role of anaerobic respiration in bread making.
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Objective: Observe cell respiration in yeast. Background: Respiration is the process by which cells take in oxygen and release carbon dioxide and energy. It is a step-by-step breakdown of glucose molecules in which energy is released. Respiration happens in all living cells, and it can occur both day and night. The equation for cellular respiration is: C 6 H 12 O 6 + 6O 2 6CO 2 + 6H 2 0 + energy (Glucose + Oxygen yields Carbon Dioxide + Water + Energy) You may notice that the equation for cellular respiration is the opposite of the equation for photosynthesis. Most cells carry out the process of cellular respiration using oxygen ( aerobic ); however, some are anaerobic. Anaerobic organisms do not use oxygen to break down glucose or have limited supplies in which to carry out this task. The energy released from glucose is converted to ATP and is required for all cellular functions for the survival of the cell/organism. The organelle, where aerobic respiration takes place in the cell, is the mitochondrion. Anaerobic respiration (fermentation) takes place in the cytoplasm. In this investigation you will work with yeast. Yeast is a one-celled fungus that reproduces rapidly under the right conditions with an ample food supply. Materials: ll, 6”–7” size)
Diameter of Balloon at Two Minute Intervals
Time (minutes) Diameter (in cm)
2 3
4 5
6 8
8 12
10 13
12 15
14 16
16 17
18 19
20 22
Data Analysis: Make a line graph of your data. Remember to keep in mind the differences between the independent and
dependent variable when graphing, and don’t forget to provide a title and label your axes.
Analysis and Conclusion: