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Phage Display by Creative Biolabs: Antibody Discovery & Interaction Studies, Study Guides, Projects, Research of Biology

Biola UniversityBiology

Creative biolabs is a leading provider of phage display technologies and services, specializing in high-quality library construction and custom screening for various project objectives. Their expertise includes antibody discovery, peptidome discovery, and specific library construction. Phage display is a powerful technique for studying protein-protein, protein-peptide, and protein-dna interactions, involving the display of the protein of interest on the surface of phages for library interrogation. Creative biolabs offers tailored phage display systems (m13, t4, or t7) and utilizes novel biopanning strategies to detect interactions and isolate high-affinity binders. Antibody libraries are constructed using variable domain coding sequences, and scfv libraries are genetically more stable than fab libraries. Phage display libraries have advantages over traditional hybridoma methods for discovering novel monoclonal antibodies and even fully human antibodies.

Typology: Study Guides, Projects, Research

2017/2018

Uploaded on 11/19/2018

bellasmith009
bellasmith009 🇺🇸

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phage display company
•Creative Biolabs is one of the well-recognized experts who is professio
nal in applying advanced phage display technologies for a broad range
of project objectives. With years of experience, our scientists can offe
r high-quality phage display library construction and custom phage dis
play library screening services to meet our clients’ demands precisely.
Particularly, our services also involve specific antibody discovery (e.g.
PTM-specific antibody, anti-idiotype antibody, and agonistic antibody)
and peptidome discovery.
pf3

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phage display company

• Creative Biolabs is one of the well-recognized experts who is professio

nal in applying advanced phage display technologies for a broad range

of project objectives. With years of experience, our scientists can offe

r high-quality phage display library construction and custom phage dis

play library screening services to meet our clients’ demands precisely.

Particularly, our services also involve specific antibody discovery (e.g.

PTM-specific antibody, anti-idiotype antibody, and agonistic antibody)

and peptidome discovery.

  • (^) Phage display is one of the most powerful and widely used laboratory technique for the study of p rotein-protein, protein-peptide and protein-DNA interactions. This technology is mainly based on displaying the interest protein (peptides, antibodies, scaffolds or others) on the surface of employi ng phage and then be used to interrogate the constructed libraries containing millions or even billi ons of displayed phages. Theoretically, phage display is an exogenous gene expression method whi ch the gene encoding the interest protein is inserted into bacteriophage coat protein gene then di splaying the interest protein on the phage surfaces, resulting in a connection between genotype a nd phenotype.
  • Due to the different properties and advantages, Creative Biolabs is pleased to tailor the most appr opriate phage display system (M13, T4 or T7) to meet our customers’ demands. Through DNA man ipulation, numerous gene variants can be created and constructed as phage display library. Our sci entists are confident in generating high-quality libraries with the diversity of 10^8. Furthermore, to detect the interaction between displayed protein and those other molecules or isolate specific tar get binders, Creative Biolabs also utilizes the novel biopanning strategies to select high-affinity bin ders or ligands which are able to recognize the naïve targets.