PLANT TISSUE CULTURE
Plant tissue culture is defined as culturing plant seeds, organs, explants, tissues, cells or protoplasts
on a chemically defined synthetic nutrient media(liquid/suspension) under sterile and controlled
conditions of light, temperature and humidity.
Basically plant tissue culture is a method of biological research in which fragment of tissue from an
animal or plant are transferred to an artificial environment in which they can continue to survive and
function.
The culturetissue may consists ofa single cell or tissue a population of cells or a whole part of a plant.
ADVANTAGES
(i) Desired medicinal plant can be produced
(ii) Production of genetically modified plant
(iii) Help to produce new plants from a single or small part of parent plant such as seed,
embryo, root tip, shoot tip, pollen grains etc
(iv) Produce disease free plant
(v) Produce exact copies of plants
(vi) Quickly produce mature plants
(vii) Also helpful techniques for production of primary and secondary metabolites
HISTORICAL DEVELOPMENT OF PLANT TISSUE CULTURE
Firstly this technique is proposed in 1902 by Gottlielo Hoberlandt , the successfully carried out tissue
culture of mesophyll tissue of a tissue.
TYPES OF CULTURE
(i)callus culture, (ii) flower culture (complete) ,(iii) anther and pollen
culture, (iv) embryo culture,(v) seed culture,(vi) protoplast culture,(vii) leaves culture,(viii) root
culture
