a.What are the selection criteria used for your plasmid, i.e. type of antibiotic selection,
etc?
- This is a mammalian cell so kanamycin or neomycin resistance is used
b. What type of promoter is present in your plasmid? Note: there may be more than one.
You just need to ID one of them.
-One of the Promoter here is SV40 or EF-1 alpha promoter and that is Constitutive
promoter. It is used to drive constant expression of the gene of interest.
c. What is the size of your plasmid in bp?
-5958 bp
d. Identify one restriction site in the MCS that could be used for insertion of your target
GOI.
- Notl
