Download Portage BIOD 210 Genetics Module 9 Exam with accurate solutions and more Exams Genetics in PDF only on Docsity!
Portage |! BIOD |! 210 |! Genetics |!
Module |! 9 |! Exam |! with |! accurate |!
solutions
Biotechnology |! - |! Correct |! answer |! ✔DNA |! technology- |! revolution |! (what |! we |! usually |! think |! of), |! in |! vitro use |! of |! living |! organisms |! and |! products |! to |! perform |! helpful |! and |! useful |! tasks |! (selective |! breeding, |! wine, |! centuries |! back) most |! important |! achievements |! of |! DNA |! reck |! - |! Correct |! answer |! ✔basic |! research |! and |! medicine the |! biotechnology |! revolution |! began |! when |! - |! Correct |! answer |! ✔recombinant |! DNA |! two |! different |! sources |! are |! combined |! in |! vitro |! in |! the |! same |! molecule transgenic |! - |! Correct |! answer |! ✔organisms |! that |! harbor |! DNA |! from |! other |! species |! each |! of |! their |! cells |! has |! this |! genetic |! manipulation
genetic |! engineering |! - |! Correct |! answer |! ✔the |! direct |! manipulation |! of |! genes |! for |! practical |! purposes intro. |! of |! gene |! that |! makes |! insulin- |! put |! into |! host |! (bacteria) |! pumps |! out |! a |! lot |! of |! insulin |! protein how |! is |! recombining |! DNA |! possible |! - |! Correct |! answer |! ✔DNA |! is |! universal |! code |! of |! life example |! of |! transgenic |! - |! Correct |! answer |! ✔mice- |! green |! fluorescent |! protein |! from |! jellyfish GFP- |! track |! expression |! of |! other |! genes- |! interred |! in |! genes |! that |! participated |! in |! hair |! follicles, |! whenever |! promter |! was |! activated |! the |! mice |! would |! grow |! green, |! attached |! to |! genes |! of |! cancer basic |! biotechnology |! toolkit |! first |! step |! - |! Correct |! answer |! ✔nucleic |! acid |! extraction nucleic |! acid |! extraction |! - |! Correct |! answer |! ✔getting |! the |! DNA |! out |! of |! the |! cells
- |! break |! open |! (lysis)
- |! digest |! (breakdown) |! proteins |! with |! enzymes
- |! separate |! DNA |! from |! proteins |! and |! other |! cellular |! deaths
- |! precipitate |! DNA |! with |! alcohol
thermal |! cycler- |! change |! temperatures |! dramatically |! in |! a |! very |! short |! period |! of |! time three |! step |! cycle |! of |! PCR |! - |! Correct |! answer |! ✔denaturing annealing extension repeated |! over |! and |! over high |! temperatures |! are |! needed |! to |! - |! Correct |! answer |! ✔denature- |! split |! double |! helix |! appart what |! temp |! does |! this |! occur |! at |! - |! Correct |! answer |! ✔95C |! mimics |! function |! of |! helices anneal |! - |! Correct |! answer |! ✔primers |! bind |! to |! the |! complimentary |! base |! pairs |! on |! the |! target |! DNA bracket |! target |! sequence give |! DNA |! polymerase |! open |! 3 |! prime |! end |! it |! needs 20 |! rounds= |! - |! Correct |! answer |! ✔ 1 |! million |! copies DNA |! isolated |! from |! any |! source |! - |! Correct |! answer |! ✔can |! be |! amplified |! through |! PCR
they |! can |! be |! sequenced |! too rapid |! diagnostic |! tests modifying |! DNA |! - |! Correct |! answer |! ✔recombinant |! DNA |! technology gene |! cloning |! (many |! copies |! of |! a |! specific |! DNA |! sequence) What |! is |! needed |! for |! gene |! cloning |! - |! Correct |! answer |! ✔restriction |! endonucleases |! )restriction |! enzymes) |! proteins |! from |! bacteria |! that |! chop |! up |! invading |! DNA |! from |! viruses cut |! at |! specific |! sequences |! at |! DNA |! strand- |! restriction |! enzyme |! sites restriction |! site |! - |! Correct |! answer |! ✔specific |! sequences |! of |! DNA |! that |! is |! targeted |! by |! enzyme each |! restriction |! enzyme |! has |! own |! site vary |! in |! size |! 4-8 |! bp |! long palindromes- |! read |! the |! same |! forward |! and |! backwards where |! do |! cut |! DNA |! - |! Correct |! answer |! ✔both |! strands |! of |! DNA |! sugar- phosphate |! backbones sticky |! ends- |! complementary |! base |! pairs |! with |! other |! sticky |! end sealed |! with |! DNA |! ligase
2 |! main |! purposes |! of |! gene |! cloning |! - |! Correct |! answer |! ✔protein |! product copies |! of |! gene |! itself how |! to |! genetically |! engineer |! bacteria |! using |! plasmids |! - |! Correct |! answer |! ✔1) |! isolate |! gene |! of |! interest
- |! isert |! gene |! of |! interest |! into |! plasmid
- |! recombinant |! plasmid |! into |! bacterial |! cells
- |! culture |! bacteria |! and |! isolate |! gene |! product GMO |! - |! Correct |! answer |! ✔any |! organism |! that |! has |! acquired |! a |! gene |! by |! artificial |! means |! or |! has |! been |! modified |! to |! over/under |! express |! one |! of |! its |! own |! genes gene |! from |! another |! species- |! transgenic less |! fertilizer, |! water example |! of |! GMO |! - |! Correct |! answer |! ✔golden |! rice- |! healthier |! to |! eat, |! disease |! resistant, |! saline |! resistant beta |! carotene |! which |! makes |! vitamin |! A |! and |! treat |! blindness more |! than |! __% |! of |! corn |! grown |! GMO |! - |! Correct |! answer |! ✔ 85 70% |! processed |! foods |! contains |! - |! Correct |! answer |! ✔GMO
Controversy |! GMO |! - |! Correct |! answer |! ✔desirable |! phenotypes- |! not |! precise, |! traditional no |! known |! study |! of |! harm |! advantages: |! better |! for |! the |! environment, |! fewer |! chemicals |! needed, |! more |! efficient |! food |! production |! and |! safety, |! more |! nutrients, |! cheaper, |! longer |! shelf |! life objections: |! ecosystem |! effects, |! genetic |! vulnerability- |! without |! diversity |! a |! single |! disease |! could |! wipe |! out |! a |! whole |! crop non-GMO |! and |! pesticides |! - |! Correct |! answer |! ✔require |! more transfenic |! animals |! - |! Correct |! answer |! ✔models |! for |! disease express |! gene |! from |! another |! species observe |! disorder |! and |! treat |! it major |! challenge |! - |! Correct |! answer |! ✔controlling |! expression |! of |! the |! trans |! gene |! and |! dosage not |! giving |! us |! endogenous |! expression |! level bioremediation |! - |! Correct |! answer |! ✔bacteria |! or |! plants |! to |! detoxify |! pollutants |! of |! clean |! up |! environments live |! in |! most |! extreme |! environments |! and |! circumstances
cDNA |! anneals |! - |! Correct |! answer |! ✔to |! its |! complimentary |! gene |! on |! the |! microarray RED |! - |! Correct |! answer |! ✔genes |! expressed |! in |! CSF |! from |! injured |! tissues GREEN |! - |! Correct |! answer |! ✔genes |! expressed |! in |! CSF |! from |! healthy |! tissue YELLOW |! - |! Correct |! answer |! ✔genes |! expressed |! in |! equal |! amounts |! in |! injured |! and |! healthy |! tissues BLACK |! - |! Correct |! answer |! ✔genes |! that |! are |! not |! expressed |! in |! either |! sample significant |! advantage |! of |! microarray |! expression |! is |! that |! - |! Correct |! answer |! ✔one |! can |! study |! the |! action |! of |! many |! genes |! at |! once |! to |! generate |! a |! big |! picute |! of |! what |! is |! going |! on |! in |! the |! tissue |! at |! one |! time what |! is |! hope |! - |! Correct |! answer |! ✔new |! targets |! for |! therapies |! or |! prevention silencing |! DNA |! - |! Correct |! answer |! ✔blocking |! the |! expression |! of |! specific |! gene
RNA |! interference- |! RNAI |! - |! Correct |! answer |! ✔blocks |! the |! activity |! of |! specific |! mRNA |! blocks |! a |! specific |! gene morpholino |! - |! Correct |! answer |! ✔synthetic |! DNA |! molecule |! 25 |! nucleotides |! in |! length targets |! specific |! genes may |! be |! a |! way |! to |! silence |! gain |! of |! function |! mutations morphilnos |! may |! be |! - |! Correct |! answer |! ✔antibiotics |! of |! the |! future PMOs- |! phopsphordiamidate |! morpholino |! oligomer drug |! resistant |! infections, |! acinetobacter genome |! editing |! - |! Correct |! answer |! ✔new |! type |! of |! gene |! silencing target |! DNA |! instead |! of |! RNA |! and |! generate |! double |! stranded |! breaks |! so |! genes |! can |! be |! swapped |! in |! and |! out CRISPR- |! Cas9 |! system sequencing |! DNA |! - |! Correct |! answer |! ✔sequence |! entire |! human |! genome |! in |! one |! day 1000 |! per |! genome Sanger |! Sequencing |! Method |! is |! still |! used |! today |! all |! over |! the |! world DNA |! sequencing |! is |! just |! - |! Correct |! answer |! ✔replication |! in |! the |! lab
